SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-52347
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Chicken IgY Clone # N393/76
Format
Azide and BSA Free
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Product Specifications
Immunogen
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human SPTBN4 (Uniprot#: Q9H254) produced recombinantly in E. Coli
Specificity
Does not cross-react with other Beta-spectrins
Clonality
Monoclonal
Host
Chicken
Isotype
IgY
Description
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N393/76 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N393/76 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.
Scientific Data Images for SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free
![Immunohistochemistry-Paraffin: SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free [NBP3-52347]](https://resources.rndsystems.com/images/products/nbp3-52347_-immunohistochemistry-paraffin-639168417276200905.jpg "Immunohistochemistry-Paraffin: SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free [NBP3-52347]")
Immunohistochemistry-Paraffin: SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free [NBP3-52347]
Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by NBP3-52346 (the BSA Free version of this antibody) (visualized in green). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the neuronal cell body and axon initial segment (AIS) in the cerebral cortex Magnification 100X.![Immunohistochemistry-Paraffin: SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free [NBP3-52347]](https://resources.rndsystems.com/images/products/nbp3-52347_-immunohistochemistry-paraffin-639168417276160123.jpg "Immunohistochemistry-Paraffin: SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free [NBP3-52347]")
Immunohistochemistry-Paraffin: SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free [NBP3-52347]
Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by NBP3-52346 (the BSA Free version of this antibody) (visualized in green). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the neuronal cell body and axon initial segment (AIS) in the cerebral cortex Magnification 400X.Applications for SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPTBN4
Alternate Names
beta-IV spectrin, KIAA1642, QV, spectrin beta chain, brain 3, spectrin, beta, non-erythrocytic 4, spectrin, non-erythroid beta chain 3, SPNB4, SPTBN3
Gene Symbol
SPTBN4
Additional SPTBN4 Products
Product Documents for SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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