SQRDL Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84510

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSPVADAAGWVDVDKETLQHRRYPNVFGIGDCTNLPTSKTAAAVAAQSGILDRTISVIMKNQTPTKKYDGYTSCPLVTGYNRVILAEFDYKAEPLETFPFDQSKERLSMYLMKADLMPFLYWNMMLRGYWG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SQRDL Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SQRDL Antibody [NBP1-84510]

Immunocytochemistry/ Immunofluorescence: SQRDL Antibody [NBP1-84510]

Immunocytochemistry/Immunofluorescence: SQRDL Antibody [NBP1-84510] - Staining of human cell line A549 shows localization to mitochondria. Antibody staining is shown in green.
SQRDL Antibody - BSA Free Western Blot: SQRDL Antibody - BSA Free [NBP1-84510]

Western Blot: SQRDL Antibody - BSA Free [NBP1-84510]

Analysis in mouse cell line NIH-3T3.
SQRDL Antibody - BSA Free Western Blot: SQRDL Antibody - BSA Free [NBP1-84510]

Western Blot: SQRDL Antibody - BSA Free [NBP1-84510]

Analysis in human cell lines A-549 and HEK293 using Anti-SQRDL antibody. Corresponding SQRDL RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
SQRDL Antibody - BSA Free Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Analysis in human colon and placenta tissues. Corresponding RNA-seq data are presented for the same tissues.
SQRDL Antibody - BSA Free Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Staining of human lymphoid tissues shows strong positivity in cytoplasm granular in non-germinal center cells.
SQRDL Antibody - BSA Free Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Staining of human placenta shows very weak granular cytoplasm positvity in trophoblastic cells.
SQRDL Antibody - BSA Free Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Staining of human prostate shows strong granular cytoplasm positivity in glandular cells.
SQRDL Antibody - BSA Free Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Staining of human rectum shows strong granular cytoplasm positivity in glandular cells.
SQRDL Antibody - BSA Free Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Immunohistochemistry-Paraffin: SQRDL Antibody [NBP1-84510]

Staining of human colon shows strong granular cytoplasm positivity in glandular cells.

Applications for SQRDL Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SQRDL

SQRDL catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex

Alternate Names

CGI-44, sulfide dehydrogenase like, sulfide quinone reductase-like (yeast), sulfide:quinone oxidoreductase, mitochondrial

Gene Symbol

SQOR

Additional SQRDL Products

Product Documents for SQRDL Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SQRDL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SQRDL Antibody - BSA Free

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Protocols

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