SR-BI Antibody - BSA Free

A: SR-BI antibody NB400-101 has been validated for Block/Neutralize, while NB400-104 has not been.

A: We denature all of our proteins using a standard protocol of 95 degrees for 10 minutes in 5% BME on all of our samples. Here is the rest of the Western Blot protocol used specifically for this antibody. 1. Run approximately 50 ug of protein on a 4-20% Tris-glycine mini-gel at 125V for 60 minutes. 2. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes. 3. Transfer protein to the membrane at 25V for 90 minutes. 4. Allow membrane to air-dry. 5. Block membrane with 1XPBS/5% non-fat milk/0.1% Tween-20 for 1 hour at room temperature (approx. 23-27 degrees C). 6. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05% Tween-20 (PBST). 7. Incubate membrane with 1:1,000 dilution of NB400-101 (anti-SR-BI), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 8. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 9. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 10. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 11. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts (NB800-PC3) were used as a positive control for this antibody.

A: Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.

A: Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.

A: SR-BI antibody NB400-101 has been validated for Block/Neutralize, while NB400-104 has not been.

A: We denature all of our proteins using a standard protocol of 95 degrees for 10 minutes in 5% BME on all of our samples. Here is the rest of the Western Blot protocol used specifically for this antibody. 1. Run approximately 50 ug of protein on a 4-20% Tris-glycine mini-gel at 125V for 60 minutes. 2. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes. 3. Transfer protein to the membrane at 25V for 90 minutes. 4. Allow membrane to air-dry. 5. Block membrane with 1XPBS/5% non-fat milk/0.1% Tween-20 for 1 hour at room temperature (approx. 23-27 degrees C). 6. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05% Tween-20 (PBST). 7. Incubate membrane with 1:1,000 dilution of NB400-101 (anti-SR-BI), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 8. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 9. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 10. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 11. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts (NB800-PC3) were used as a positive control for this antibody.

A: Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.

A: Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.

A: SR-BI antibody NB400-101 has been validated for Block/Neutralize, while NB400-104 has not been.

A: We denature all of our proteins using a standard protocol of 95 degrees for 10 minutes in 5% BME on all of our samples. Here is the rest of the Western Blot protocol used specifically for this antibody. 1. Run approximately 50 ug of protein on a 4-20% Tris-glycine mini-gel at 125V for 60 minutes. 2. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes. 3. Transfer protein to the membrane at 25V for 90 minutes. 4. Allow membrane to air-dry. 5. Block membrane with 1XPBS/5% non-fat milk/0.1% Tween-20 for 1 hour at room temperature (approx. 23-27 degrees C). 6. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05% Tween-20 (PBST). 7. Incubate membrane with 1:1,000 dilution of NB400-101 (anti-SR-BI), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 8. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 9. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 10. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 11. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts (NB800-PC3) were used as a positive control for this antibody.

A: Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.

A: Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.

A: SR-BI antibody NB400-101 has been validated for Block/Neutralize, while NB400-104 has not been.

A: We denature all of our proteins using a standard protocol of 95 degrees for 10 minutes in 5% BME on all of our samples. Here is the rest of the Western Blot protocol used specifically for this antibody. 1. Run approximately 50 ug of protein on a 4-20% Tris-glycine mini-gel at 125V for 60 minutes. 2. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes. 3. Transfer protein to the membrane at 25V for 90 minutes. 4. Allow membrane to air-dry. 5. Block membrane with 1XPBS/5% non-fat milk/0.1% Tween-20 for 1 hour at room temperature (approx. 23-27 degrees C). 6. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05% Tween-20 (PBST). 7. Incubate membrane with 1:1,000 dilution of NB400-101 (anti-SR-BI), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 8. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 9. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 10. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 11. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts (NB800-PC3) were used as a positive control for this antibody.

A: Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.

A: Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.