SRPX2 Antibody - BSA Free

Western Blot: SRPX2 Antibody - BSA Free [NBP1-77370] -

Differentially expressed mRNAs in the hypertensive aorta as determined by microarray analysis. To identify differentially expressed genes in the hypertensive arterial wall, we isolated the aorta from vehicle (physiological saline or NaCl, n = 7, mean arterial blood pressure (MAP): 103 +/- 11 mmHg) and angiotensin II (AngII)-treated mice (n = 8, MAP: 186 +/- 6 mmHg) and subjected the isolated RNA to microarray analysis. 235 genes were differentially expressed (SAM analysis, Q = 0). Panels A and B show the ten most upregulated and the ten most repressed mRNAs, respectively. All loading controls used herein for western blotting changed less than 10%, and non-significantly (Hsp90: q = 11; Gapdh: q = 51; Actb: q = 35), in the array experiment. 14 genes were chosen for confirmation by RT-qPCR in a material that included the original samples plus four samples in each group that were not included in the array experiment. Six of the mRNA changes, including those for Gucy1a3 and Gucy1b3 could be confirmed (*P < 0.05, **P < 0.01, P < 0.001). In panel C the fold change in the qRT-PCR experiment is plotted versus the fold change in the array experiment. A highly significant correlation (Spearman method, P < 0.0001) was seen. Western blotting was done in (D) using aortic protein lysates (n = 6/group) to assess changes for a selection of the genes in (C). To allow for detection of multiple targets, membranes were cut horizontally above and below the known molecular weights and hence full lane blots are not available. Panels E and F show summarized data at the protein level for the subunits (GUCY1A3, GUCY1B3) of the soluble guanylyl cyclase (sGC). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28465505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.