ST2/IL-33R Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85251

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ST2/IL-33R Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PDVLENKCGYTLCIYGRDMLPGEDVVTAVETNIRKSRRHIFILTPQITHNKEFAYEQEVALHCALIQNDAKVILIEMEALSELDMLQAEALQDSLQHLMKVQGTIKWREDHIANKRSLNSKFWKHVRYQMPVPSKIPRKASSLTPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ST2/IL-33R Antibody - BSA Free

Western Blot: ST2/IL-33R Antibody [NBP1-85251]

Western Blot: ST2/IL-33R Antibody [NBP1-85251]

Western Blot: ST2/IL-33 R Antibody [NBP1-85251] - Analysis in human cell line HEL.
Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]

Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]

Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]

Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]

Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human stomach shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]

Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]

Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]

Immunohistochemistry-Paraffin: ST2/IL-33R Antibody [NBP1-85251]

Immunohistochemistry-Paraffin: ST2/IL-33 R Antibody [NBP1-85251] - Staining of human lung shows moderate membranous positivity in pneumocytes.

Applications for ST2/IL-33R Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-85251 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ST2/IL-33R

The protein encoded by the IL1RL1 gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. Alternative splicing of this gene results in multiple transcript variants. (provided by RefSeq)

Long Name

Interleukin 33 Receptor

Alternate Names

DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1

Gene Symbol

IL1RL1

Additional ST2/IL-33R Products

Product Documents for ST2/IL-33R Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ST2/IL-33R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Group 2 ILCs
IL-1 Family

Citations for ST2/IL-33R Antibody - BSA Free

Customer Reviews for ST2/IL-33R Antibody - BSA Free (1)

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Customer Images

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  • Name: Amr Mahmoud
    Application: Immunohistochemistry-Paraffin
    Sample Tested: FFPE
    Species: Human
    Verified Customer | Posted 01/07/2015
    Antibody did not stain skeletal muscle, as described in the literature
    ST2/IL-33R Antibody - BSA Free NBP1-85251

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Protocols

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FAQs for ST2/IL-33R Antibody - BSA Free

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  • Q: Does this antibody recognize a specific isoform for ST2, particularly ST2 L or not?

    A: The immunogen sequence for IL1RL1 Antibody (NBP1-85251) is stated on its datasheet. I have compared this for you with the ST2 isoforms as stated on UniProt. I can tell you that the immunogen matches perfectly with ST2L, and not with ST2S or ST2V. This is because the immunogen sequence corresponds to a region close to the C terminus of ST2L, within the sections missing in ST2S and ST2V (according to UniProt).

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