StAR Antibody (HL1360) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25696

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Canine, Feline

Predicted:

Porcine (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1360 expressed in HEK293

Format

Azide and BSA Free
View all StAR Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human StAR. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%), Sheep (86%), Bovine (87%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for StAR Antibody (HL1360) - Azide and BSA Free

StAR Antibody (HL1360) - Azide and BSA Free

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] -

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
StAR Antibody (HL1360) - Azide and BSA Free

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] -

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
StAR Antibody (HL1360) - Azide and BSA Free

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] -

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
StAR Antibody (HL1360) - Azide and BSA Free

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] -

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
StAR Antibody (HL1360) - Azide and BSA Free

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] -

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
StAR Antibody (HL1360) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] -

Immunocytochemistry/Immunofluorescence: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:500. Blue: Fluoroshield with DAPI.
StAR Antibody (HL1360) - Azide and BSA Free

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] -

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
StAR Antibody (HL1360) - Azide and BSA Free

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] -

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for StAR Antibody (HL1360) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: StAR

StAR is encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Alternate Names

cholesterol trafficker, mitochondrial steroid acute regulatory protein, StAR, StARD1, STARD1StAR-related lipid transfer (START) domain containing 1, START domain containing 1, START domain-containing protein 1, steroid acute regulatory protein, steroidogenic acute regulator, steroidogenic acute regulatory protein, steroidogenic acute regulatory protein, mitochondrial

Gene Symbol

STAR

Additional StAR Products

Product Documents for StAR Antibody (HL1360) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for StAR Antibody (HL1360) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for StAR Antibody (HL1360) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review StAR Antibody (HL1360) - Azide and BSA Free and earn rewards!

Have you used StAR Antibody (HL1360) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies