STAT1 Antibody (G3-B11-R)

Western Blot: STAT1 Antibody (G3-B11-R) [NBP3-32980]

Western Blot: STAT1 Antibody (G3-B11-R) [NBP3-32980] - Western blot analysis of STAT1 on different lysates with Mouse anti-STAT1 antibody (NBP3-32980) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: A431 cell lysate
Lane 3: HeLa cell lysate
Lane 4: A549 cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: SK-MEL-28 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: HT-29 cell lysate

Lysates/proteins at 15 ug/Lane.

Predicted band size: 87/83 kDa
Observed band size: 87/83 kDa

Exposure time: 30 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32980) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: STAT1 Antibody (G3-B11-R) [NBP3-32980]

Immunohistochemistry: STAT1 Antibody (G3-B11-R) [NBP3-32980] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-STAT1 antibody (NBP3-32980) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32980) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.