STAT4 Antibody (1C2-1C12) - Azide and BSA Free

Novus Biologicals | Catalog # H00006775-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C2-1C12

Format

Azide and BSA Free
View all STAT4 Primary Antibodies »

Product Specifications

Immunogen

STAT4 (AAH31212, 1 a.a. ~ 748 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE

Specificity

STAT4 - signal transducer and activator of transcription 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for STAT4 Antibody (1C2-1C12) - Azide and BSA Free

Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01] - STAT4 monoclonal antibody (M01), clone 1C2-1C12. Analysis of STAT4 expression in human liver.
Immunocytochemistry/ Immunofluorescence: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Immunocytochemistry/ Immunofluorescence: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Immunocytochemistry/Immunofluorescence: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Analysis of monoclonal antibody to STAT4 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Immunohistochemistry-Paraffin: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Immunohistochemistry-Paraffin: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Analysis of monoclonal antibody to STAT4 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 1 ~ 10 ug/ml.
Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01] - STAT4 monoclonal antibody (M01), clone 1C2-1C12 Analysis of STAT4 expression in A-549.
Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Western Blot: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Analysis of STAT4 expression in transfected 293T cell line by STAT4 monoclonal antibody (M01), clone 1C2-1C12.Lane 1: STAT4 transfected lysate(85.9 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Immunoprecipitation: STAT4 Antibody (1C2-1C12) [H00006775-M01]

Immunoprecipitation: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Analysis of STAT4 transfected lysate using anti-STAT4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STAT4 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: STAT4 Antibody (1C2-1C12) [H00006775-M01] - Detection limit for recombinant GST tagged STAT4 is approximately 0.3ng/ml as a capture antibody.

Applications for STAT4 Antibody (1C2-1C12) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recominant protein, tissue lysate and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STAT4

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Long Name

Signal Transducer and Activator of Transcription 4

Alternate Names

signal transducer and activator of transcription 4, SLEB11

Entrez Gene IDs

6775 (Human)

Gene Symbol

STAT4

OMIM

600558 (Human)

UniProt

AAH31212 (Human)

Additional STAT4 Products

Product Documents for STAT4 Antibody (1C2-1C12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for STAT4 Antibody (1C2-1C12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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