STAT5b Antibody (STAT5B/2611)

Name: STAT5b Antibody (STAT5B/2611)
Brand: Novus Biologicals
SKU: NBP2-79773
Price: 209 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-79773

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # STAT5B/2611

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Product Specifications

Immunogen

Recombinant full-length human STAT5b protein (Uniprot: P51692)

Localization

Cytoplasm & Nucleus. Translocated into the nucleus in response to phosphorylation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79907)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for STAT5b Antibody (STAT5B/2611)

Immunohistochemistry-Paraffin: STAT5b Antibody (STAT5B/2611) [NBP2-79773]

Immunohistochemistry-Paraffin: STAT5b Antibody (STAT5B/2611) [NBP2-79773] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with STAT5b Antibody (STAT5B/2611).

Protein Array: STAT5b Antibody (STAT5B/2611) [NBP2-79773]

Protein Array: STAT5b Antibody (STAT5B/2611) [NBP2-79773] - Analysis of Protein Array containing >19,000 full-length human proteins using Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for STAT5b Antibody (STAT5B/2611)

Application

Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: STAT5b

STAT5B is encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

Long Name

Signal Transducer and Activator of Transcription 5b

Alternate Names

signal transducer and activator of transcription 5B, STAT5, transcription factor STAT5B

Gene Symbol

STAT5B

UniProt

P51692

Additional STAT5b Products

Product Documents for STAT5b Antibody (STAT5B/2611)

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Product Specific Notices for STAT5b Antibody (STAT5B/2611)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.