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Stathmin-2/STMN2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-49461

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse, Rat, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, In vitro assay, In vivo assay, Knockdown Validated

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, In vivo assay, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Stathmin-2/STMN2 Primary Antibodies »

Product Specifications

Immunogen

C-terminal peptide of mouse STMN2. [Swiss-Prot P55821]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32778834).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Stathmin-2/STMN2 Antibody - BSA Free

Knockdown Validation of Stathmin-2/STMN2 Antibody in Mouse DRG Neurons by Western Blot

Knockdown Validation of Stathmin-2/STMN2 Antibody in Mouse DRG Neurons by Western Blot

Analysis of endogenous STMN2 in mouse dorsal root ganglia (DRG) neurons. A partial siRNA knockdown was used in the second lane. NBP1-49461 was used at a dilution of 1:1000. Image courtesy of Dr. Jung Eun Shin.
Immunocytochemistry/Immunofluorescence Analysis of Stathmin-2/STMN2 in Mouse DRG Neurons

Immunocytochemistry/Immunofluorescence Analysis of Stathmin-2/STMN2 in Mouse DRG Neurons

Staining of STMN2 in primary mouse dorsal root ganglia (DRG) neurons Image shows the expected staining of endogenous STMN2 in axons and growth cones. Tubulin is a marker of axons. NBP1-49461 was used at a dilution of 1:4000. Image courtesy of Dr. Jung Eun Shin.
Immunohistochemical Staining of Stathmin-2/STMN2 in Frozen Mouse Sciatic Nerves

Immunohistochemical Staining of Stathmin-2/STMN2 in Frozen Mouse Sciatic Nerves

Stathmin-2-STMN2-Antibody-Immunohistochemistry-Frozen-NBP1-49461-img0015.jpg
Immunocytochemistry/Immunofluorescence Staining of Stathmin-2/STMN2 in Transfected HEK293T Cells

Immunocytochemistry/Immunofluorescence Staining of Stathmin-2/STMN2 in Transfected HEK293T Cells

Staining of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:1000 (A, B) and 1:3000 (C). Image courtesy of Dr. Jung Eun Shin.
Western Blot Analysis of Stathmin-2/STMN2 in Transfected HEK293T Cells

Western Blot Analysis of Stathmin-2/STMN2 in Transfected HEK293T Cells

Analysis of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:4000. Image courtesy of Dr. Jung Eun Shin.
Immunohistochemical Detection of Stathmin-2/STMN2 in Frozen Mouse Trigeminal Root Entry Zone

Immunohistochemical Detection of Stathmin-2/STMN2 in Frozen Mouse Trigeminal Root Entry Zone

Stathmin-2/STMN2 immunoreactivity at the trigeminal root entry zone (antibody diluted 1:1000, tissue taken from a mouse with experimental autoimmune encephalomyelitis). Image from verified customer review.

Applications for Stathmin-2/STMN2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry Whole-Mount

reported in scientific literature (PMID 35042776)

Immunohistochemistry-Frozen

1:200 - 1:500

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 31182472)

In vitro assay

reported in scientific literature (PMID 22726832)

In vivo assay

reported in scientific literature (PMID 22726832)

Western Blot

1 - 2 ug/ml
Application Notes
In Western blot a band can be seen at ~22 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-49461 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Stathmin-2/STMN2

STMN2 (Stathmin-2 or SCG10/Scgn10) belongs to stathmin family of proteins which are known to bind tubulin for acting as a sequestering agents and for promoting microtubule disassembly. STMN2 is a membrane-associated neuronal protein expressed mainly during development and its expression correlates with neurite outgrowth. STMN2 possesses a unique N-terminal domain that is critical for membrane binding, is responsible for STMN2 localization to the Golgi complex and is important in its targeting to growth cones. STMN2 localize to perinuclear cytoplasm, axons, and growth cones of developing neurons, and exists in soluble as well as membrane-bound forms. STMN2 interacts with ITMC2, as well as MAPK8, and when phosphorylated by MAPK8, it act as regulator of microtubule stability which controls neurite length in cortical neurons. Unlike other stathmins, STMN2 acts in two ways to promote microtubule dynamics: it stabilizes microtubules at their plus end and promotes microtubule catastrophe at their minus end. In the developing brain, STMN2 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Reduced STMN2 expression is associated with Down's syndrome and Alzheimer's disease.

Alternate Names

SCG10, SCGN10, Stathmin2, STMN2

Gene Symbol

STMN2

Additional Stathmin-2/STMN2 Products

Product Documents for Stathmin-2/STMN2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Stathmin-2/STMN2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Microtubules

Citations for Stathmin-2/STMN2 Antibody - BSA Free

Customer Reviews for Stathmin-2/STMN2 Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Stathmin-2/STMN2 Antibody
    Name: Kevin Thorburn
    Application: Immunohistochemistry-Frozen
    Sample Tested: mouse trigeminal root entry zone and trigeminal root entry zone
    Species: Mouse
    Verified Customer | Posted 11/29/2018
    Stathmin-2/STMN2 immunoreactivity at the trigeminal root entry zone (antibody diluted at 1:1000, tissue taken from a mouse with experimental autoimmune encephalomyelitis)
    Stathmin-2/STMN2 Antibody - BSA Free NBP1-49461
  • Stathmin-2/STMN2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: sciatic nerve
    Species: Other
    Verified Customer | Posted 08/31/2016
    SCG10 (red) labeling regenerating fibers in mouse sciatic nerve

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Protocols

View specific protocols for Stathmin-2/STMN2 Antibody - BSA Free (NBP1-49461):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.


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