STIM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84322

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all STIM1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFK

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24621671).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STIM1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: STIM1 Antibody [NBP1-84322]

Immunocytochemistry/ Immunofluorescence: STIM1 Antibody [NBP1-84322]

Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NBP1-84322] - Staining of human cell line A549 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Western Blot: STIM1 Antibody [NBP1-84322]

Western Blot: STIM1 Antibody [NBP1-84322]

Western Blot: STIM1 Antibody [NBP1-84322] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts..
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-STIM1 antibody NBP1-84322 (A) shows similar protein distribution across tissues to independent antibody NBP1-84323 (B).
STIM1 Antibody - BSA Free Western Blot: STIM1 Antibody - BSA Free [NBP1-84322]

Western Blot: STIM1 Antibody - BSA Free [NBP1-84322]

Analysis in human cell lines A-549 and MCF-7 using Anti-STIM1 antibody. Corresponding STIM1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for STIM1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24621671). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STIM1

Stromal interaction molecule 1 plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. It acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, it translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit.

Long Name

Stromal Interaction Molecule 1

Alternate Names

GOK

Gene Symbol

STIM1

Additional STIM1 Products

Product Documents for STIM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for STIM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

Citations for STIM1 Antibody - BSA Free

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Protocols

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