STK39 Antibody (0D9L5)

Novus Biologicals | Catalog # NBP3-16104

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0D9L5 expressed in HEK293
View all STK39 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 401-545 of human STK39 (Q9UEW8). SQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STK39 Antibody (0D9L5)

Western Blot: STK39 Antibody (0D9L5) [NBP3-16104]

Western Blot: STK39 Antibody (0D9L5) [NBP3-16104]

Western Blot: STK39 Antibody (0D9L5) [NBP3-16104] - Western blot analysis of extracts of Mouse testis, using STK39 Rabbit mAb (NBP3-16104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: STK39 Antibody (0D9L5) [NBP3-16104]

Western Blot: STK39 Antibody (0D9L5) [NBP3-16104]

Western Blot: STK39 Antibody (0D9L5) [NBP3-16104] - Western blot analysis of extracts of various cell lines, using STK39 Rabbit mAb (NBP3-16104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: STK39 Antibody (0D9L5) [NBP3-16104]

Immunohistochemistry-Paraffin: STK39 Antibody (0D9L5) [NBP3-16104]

Immunohistochemistry-Paraffin: STK39 Antibody (0D9L5) [NBP3-16104] - Immunohistochemistry of paraffin-embedded human placenta using STK39 Rabbit mAb (NBP3-16104) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
STK39 Antibody (0D9L5)

Immunocytochemistry/ Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104] -

Immunocytochemistry/ Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104] - Confocal imaging of NIH/3T3 cells using STK39 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
STK39 Antibody (0D9L5)

Immunocytochemistry/ Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104] -

Immunocytochemistry/ Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104] - Confocal imaging of Hep G2 cells using STK39 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). DAPI was used for nuclear staining (blue). Objective: 100x.
STK39 Antibody (0D9L5)

Immunocytochemistry/ Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104] -

Immunocytochemistry/ Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104] - Confocal imaging of human colon using STK39 Rabbit mAb. Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Applications for STK39 Antibody (0D9L5)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STK39

STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Alternate Names

DCHTsmall intestine SPAK-like kinase, DKFZp686K05124, EC 2.7.11, EC 2.7.11.1, PASK, proline-alanine-rich STE20-related kinase, serine threonine kinase 39, Serine/threonine-protein kinase 39, SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast), STE20/SPS1 homolog, STE20/SPS1-related proline-alanine-rich protein kinase, Ste20-like protein kinase, Ste-20-related kinase

Gene Symbol

STK39

Additional STK39 Products

Product Documents for STK39 Antibody (0D9L5)

Certificate of Analysis

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Product Specific Notices for STK39 Antibody (0D9L5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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