STRAP Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-20521
![Knockout Validated: STRAP Antibody [NBP2-20521]](https://resources.rndsystems.com/images/products/STRAP-Antibody-Western-Blot-NBP2-20521-img0011.jpg "Western Blot: STRAP Antibody [NBP2-20521]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (99%), Chicken (90%). Backed by our 100% Guarantee.
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human STRAP. The exact sequence is proprietary.
Reactivity Notes
Zebrafish (82%), Xenopus laevis (84%).
Localization
Cytoplasm; Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for STRAP Antibody - BSA Free
Western Blot: STRAP Antibody [NBP2-20521]
Western Blot: STRAP Antibody [NBP2-20521] - Wild-type (WT) and STRAP knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: STRAP Antibody [NBP2-20521] -
Western Blot: STRAP Antibody [NBP2-20521] - STRAP antibody [N1C3] detects STRAP protein by western blot analysis.A. 30 ug MCF-7 whole cell lysate/extract
B. 30 ug MDA-MB-231 whole cell lysate/extract
10% SDS-PAGE
STRAP antibody [N1C3] (NBP2-20521) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: STRAP Antibody [NBP2-20521] -
Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (NBP2-20521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry: STRAP Antibody [NBP2-20521] -
STRAP antibody [N1C3] detects STRAP protein at cytosol on mouse stomach by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.
STRAP antibody [N1C3] (NBP2-20521) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence: STRAP Antibody [NBP2-20521] -
STRAP antibody [N1C3] detects STRAP protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STRAP protein stained by STRAP antibody [N1C3] (NBP2-20521) diluted at 1:500.
Blue: Hoechst 33342 staining.
Western Blot: STRAP Antibody [NBP2-20521] -
STRAP antibody [N1C3] detects STRAP protein by western blot analysis.A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
10% SDS-PAGE
STRAP antibody [N1C3] (NBP2-20521) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: STRAP Antibody [NBP2-20521] -
Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (NBP2-20521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for STRAP Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: STRAP
Alternate Names
MAP activator with WD repeats, MAWDpt-wd, serine/threonine kinase receptor associated protein, serine-threonine kinase receptor-associated protein, UNR-interacting protein, UNRIPPT-WD, WD-40 repeat protein PT-WD
Gene Symbol
STRAP
UniProt
Additional STRAP Products
Product Documents for STRAP Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for STRAP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for STRAP Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for STRAP Antibody - BSA Free
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Q: I am looking to buy an antibody for STRAP (serine-threonine receptor associated protein) and was wondering if you had published references related to the use of your STRAP antibodies. My application would be for Western blot.
A: Unfortunately, we do not have any published references related to the use of our STRAP antibodies yet. The antibodies were tested and validated and the images published on our website are in house images. If you would like me to help you choose an antibody that would be the best choice for your purposes please let me know what species you are working with. I also wanted to mention our 100% Novus Guarantee to you. We guarantee that the antibody you will use will work in species and application we have validated it in. If you experience any troubles, we will be happy to troubleshoot with you. However, should we fail to resolve your problems we will refund your purchase or replace the antibody with a more appropriate substitute.
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