Surfactant Protein A Antibody

Novus Biologicals | Catalog # NBP2-12928

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SFTPA1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HLDEELQATLHDFR corresponding to internal region according to NP_005402.3, NP_001087239.2, NP_001158117.1, NP_001158118.1, NP_001092138.1.

Epitope

CHLDEELQATLHDFR

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine. Use in Mouse reported in scientific literature (PMID:32157214).

Specificity

This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Surfactant Protein A Antibody

Western Blot: Surfactant Protein A Antibody [NBP2-12928]

Western Blot: Surfactant Protein A Antibody [NBP2-12928]

Western Blot: Surfactant Protein A Antibody [NBP2-12928] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928]

Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928]

Surfactant-Protein-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-12928-img0005.jpg
Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-12928]

Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-12928]

Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-12928] - Staining of paraffin embedded Human Lung. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928]

Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928]

Surfactant-Protein-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-12928-img0003.jpg
Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928]

Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928]

Surfactant-Protein-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-12928-img0004.jpg
Surfactant Protein A Antibody

Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] -

Immunocytochemistry/ Immunofluorescence: Surfactant Protein A Antibody [NBP2-12928] - ECM1-alpha 6 beta 4-ABCG1 axis is enriched in the S/TB region of the lungs in patients with pneumonia.IF analysis of GPRC5A, SPA/ABCG1, alpha 6/ beta 4, alpha 6/ECM1 & beta 4/ECM1 in normal or pneumonic lung tissue samples (a) & the calculation of positive or dual-positive cells in the S/TB region (b), (Bar = 100 µm); c Graphical abstract of the study: GPRC5A deficiency promotes the activation of NF-kB & subsequent expression & secretion of ECM1; the secreted ECM1 interacts with alpha 6 beta 4 of AT2 cells & induces the activation of NF-kB, which induces the expression of ABCG1. AT2 cells with ABCG1 expression are one of the originating cell populations of lung cancer. Data were collected from three independent experiments with triplicate samples. **P < 0.01; ***P < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32157214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Surfactant Protein A Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human lung lysates (calculated MW of 27.7 kDa band according to NP_001087239.2). Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32157214).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SFTPA1

Surfactant protein A is a protein with two isoforms, with lengths of 248 and 263 amino acids and weights of approximately 26 and 28 kDa respectively. Surfactant protein A binds to surfactant phospholipids in the presence of calcium ions and helps lower the surface tension in the alveoli of the lungs. Current research is being done on several diseases and disorders linked to this protein including pulmonary fibrosis, allergic bronchopulmonary aspergillosis, pulmonary alveolar proteinosis, non-small cell lung carcinoma, sclerosing hemangioma, otitis media, chronic obstructive pulmonary disease, bronchitis obliterans, bronchopulmonary dysplasia, rhinitis, aspergillosis, pulmonary edema, insulin resistance, and pneumoconiosis. Surfactant protein A has also been shown to have interactions with DMBT1, MYO18A, C1QA, SFTPD, and TLR2 in pathways such as the immune response, bacterial infections in CF airways, cell-cell communication, phagosome, and pertussis pathways.

Long Name

Surfactant Protein A1

Alternate Names

COLEC4, PSP-A, SP-A, SP-A1

Entrez Gene IDs

653509 (Human)

Gene Symbol

SFTPA1

Additional SFTPA1 Products

Product Documents for Surfactant Protein A Antibody

Certificate of Analysis

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Product Specific Notices for Surfactant Protein A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Surfactant Protein A Antibody

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Protocols

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