Synapsin I Antibody - Azide Free

Novus Biologicals | Catalog # NB300-104

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig, Primate

Cited:

Human, Mouse, Rat, Guinea Pig, Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Synapsin I Primary Antibodies »

Product Specifications

Immunogen

Native protein purified from bovine brain. Accession # P17599

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 20384782). Guinea Pig reactivity reported in scientific literature (PMID: 25339870).

Localization

Synapse, Synaptic vesicle, Golgi apparatus

Marker

pre-Synaptic Marker

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)

Scientific Data Images for Synapsin I Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104]

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104]

Synapsin-I-Antibody-Immunocytochemistry-Immunofluorescence-NB300-104-img0013.jpg
Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104]

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104]

Synapsin-I-Antibody-Immunocytochemistry-Immunofluorescence-NB300-104-img0014.jpg
Western Blot: Synapsin I Antibody [NB300-104]

Western Blot: Synapsin I Antibody [NB300-104]

Synapsin I Antibody-Western Blot-NB300-104-img0015.jpg
Immunohistochemistry-Paraffin: Synapsin I Antibody [NB300-104]

Immunohistochemistry-Paraffin: Synapsin I Antibody [NB300-104]

Immunohistochemistry-Paraffin: Synapsin I Antibody [NB300-104] - Human cerebellum section, formalin fixed paraffin embedded. Stained with Synpasin-1 at 1:250. Citrate antigen retrieval at pH 6. Image from verified customer review.
Western Blot: Synapsin I Antibody [NB300-104]

Western Blot: Synapsin I Antibody [NB300-104]

Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.
Western Blot: Synapsin I Antibody [NB300-104]

Western Blot: Synapsin I Antibody [NB300-104]

Western Blot: Synapsin I Antibody [NB300-104] - 10 ug of rat brain lysate showing specific immunolabeling of the ~78 kDa Synapsin I doublet.
Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104]

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104]

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] - ICC-IF validation analysis of Synapsin 1 Antibody on cultured rat caudate neurons. This representative image shows the punctate distribution of Synapsin 1 (green) and MAP (red) proteins in a neuronal cell.
Synapsin I Antibody

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody [NB300-104] -

Characterization of WT and slow ESCs differentiated to neurons (related to Fig 4)Immunofluorescence staining for neuronal markers Map2, Syn1, and NeuN in neurons cultured on poly‐ornithine‐/laminin‐coated plates.Expression of neuronal markers in neurons cultured on poly‐ornithine‐/laminin‐coated plates from RNA‐seq analysis, n = 3.
Synapsin I Antibody

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] -

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - Characterization of WT & slow ESCs differentiated to neurons (related to Fig 4)Immunofluorescence staining for neuronal markers Map2, Syn1, & NeuN in neurons cultured on poly‐ornithine‐/laminin‐coated plates.Expression of neuronal markers in neurons cultured on poly‐ornithine‐/laminin‐coated plates from RNA‐seq analysis, n = 3. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30988016), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Synapsin I Antibody

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] -

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody [NB300-104] - hPDLSCs-derived neural-like cells are connected by synapse-like interactions. hPDLSCs-derived neural-like cells connect to one another (a) through different types of synapses-like interactions, including dendrodendritic-like, axoaxonic-like & axodendritic-like synapses (b). (c) Synapse-associated proteins Cx43, Synaptophysin & Synapsin1 are found in the cell membrane of hPDLSCs-derived neural-like cells at the neurite contact areas. Scale bar: 25 μm. AA, axoaxonic-like synapse; AD, axodendritic-like synapse; DD, dendrodendritic-like synapse; LM, light microscopy; SEM, scanning electron microscopy. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31792338), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Synapsin I Antibody

Western Blot: Synapsin I Antibody [NB300-104] -

Western Blot: Synapsin I Antibody [NB300-104] - Synaptic localization of TA at the PC–PF synaptic contacts.Western blot of TA, V1, V2, VGAT proteins in cerebellar synaptosomal fractions (SYN) from P14 mice. Synaptosomal preparations are enriched of post synaptic density protein (PSD95) & Synapsin-I (Syn-I) in comparison to equal amount (ACT) of total cerebellar protein extract (LYS). TA is clearly present in synaptosomal enriched preparations. B) Cerebellar sagittal section immunostained for Cb (green), V1 (cyan) & TA (red). In the ML, anti-V1 specifically labels PF-terminals contacting PC-spines. C–E) The high magnification of the inset in B is reproduced in different merge images & by the relative split channels. C) Merge image of Cb & V1 staining plus the colocalization mask Cb/V1 (mCb/V1 in white); it represents the negative CTR (CTR-). D) Merge image of TA & Cb staining plus the colocalization mask TA/Cb (mTA/Cb in white), highlighting TA expression in PC-spines. E) Merge image of TA & V1 staining plus the colocalization mask TA/V1 (m TA/V1 in white), indicating the presence of TA also in this synaptic terminal. F) Quantitative colocalization analysis shows the mean overlap coefficients of TA/Cb (gray column) & TA/V1 (black column) significantly different from the negative CTR Cb/V1 (white column). The small insets (white boxes in C–D-E) are high magnifications of a representative PC-spine (green) contacted by a PF synaptic terminal (cyan) with the appropriate colocalization mask (white). * p < 0.05. Data are represented as mean ± SEM. Scale bars in B, 10 µm; in C–D-E, 5 µm; in F, 1 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23840813), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Synapsin I Antibody

Western Blot: Synapsin I Antibody [NB300-104] -

Western Blot: Synapsin I Antibody [NB300-104] - Tau oligomers induce synaptic dysfunction. (A) Representative Western blot of mouse hippocampus homogenate. The levels of synaptophysin, synapsin-1, & septin-11 were measured by band quantification & normalized with the levels of tubulin. PBSo indicates representative bands of hippocampal area injected with PBS in mice also injected with tau oligomers, PBSf indicates PBS injection in mice also injected with tau fibrils, & PBSm indicates PBS injection in mice also injected with tau monomers. (B) Synaptophysin levels were significantly lower in the hemispheres injected with tau oligomers in comparison with the ones injected with fibrils, monomers, or PBS. (C) No significant differences in the levels of synapsin-1 were observed. (D) Only the hemisphere injected with tau oligomers presents a decrease in the level of septin-11. Data are represented as the mean ± SE. *p < 0.01, n = 6 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21645391), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Synapsin I Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1 ug per 200ug lysate

Western Blot

1:1000
Application Notes
NB 300-104 can be used in Western blot where a doublet is seen at ~ 80 kDa representing synapsin Ia and Ib. Immunofluoresence, Immunoprecipitation (1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.) May work in IHC-Paraffin but this has not been tested. Differential distribution of the synapsins in the rat olfactory bulb. Has been reported in the literature to work in WB (rat cortex), IHC (cryosections) on rat olfacotry bulb. (PMID: 8283238) Use in Immunohistochemistry free floating reported in multiple pieces of scientific literature.

Reviewed Applications

Read 1 review rated 5 using NB300-104 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synapsin I

Synapsin I, also called Synapsin-1 or SYN1, belongs to Synapsin family (Synapsin I, Synapsin II, Synapsin III) of evolutionarily conserved phospho-proteins which are associated with the cytosolic side of the synaptic vesicles and tether the latter to actin cytoskeleton, thus forming a reserve pool. Synapsins represents the most abundant of neuron-specific phosphor-proteins, consisting 9% of the total amount of all vesicle proteins in neurons wherein they play a critical role in the regulation of neurotransmitter release. Synapsin I is present in axon terminals and plays a role in regulating the secretion of neurotransmitters; thus, it influences synaptic plasticity by regulating pre- and post-synaptic vesicular release. Synapsins I and II are typically found in mature synapses, while synapsin III is often attributed to developing synapses with lesser overall expression. Phosphorylation at Ser-9 residue results in dissociation of Synapsins I from synaptic vesicles which is important for synaptic vesicle neurotransmitter release. Mutations in Synapsin I have been linked to Epilepsy X-linked, with variable learning disabilities and behavior disorders (XELBD) which is a neurologic disorder characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.

Alternate Names

SYN1, Synapsin I, synaptic marker, synaptic markers immunohistochemistry

Entrez Gene IDs

6853 (Human); 20964 (Mouse); 24949 (Rat)

Gene Symbol

SYN1

UniProt

P17600 (Human)

Additional Synapsin I Products

Product Documents for Synapsin I Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Synapsin I Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Citations for Synapsin I Antibody - Azide Free

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  • Synapsin I Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Brain (cerebellum) tissue
    Species: Human
    Verified Customer | Posted 04/17/2018
    Human cerebellum section, formalin fixed paraffin embedded. Stained with Synpasin-1 at 1:250. Citrate antigen retrieval used.
    Citrate antigen retrieval, pH6 1 in 250 dilution
    Synapsin I Antibody - Azide Free NB300-104

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FAQs for Synapsin I Antibody - Azide Free

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  • Q: How many days do you recommend culturing primary neurons to get a good Synapsin I signal? Can you please send the protocol used to validate this antibody in immunofluorescence?

    A: It was validated in IF by a collaborator, so I do not have a specific protocol to offer. However I can tell you that the neurons were cultured for 14 days.

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