Synaptophysin Antibody

Novus Biologicals | Catalog # NB300-653

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Feline, Monkey

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Synaptophysin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human Synaptophysin protein.

Reactivity Notes

Feline, Rat reactivity reported from a verified customer review.

Localization

Integral membrane protein. Synaptic vesicles.

Marker

Pre-synaptic marker

Specificity

This antibody does not cross-react with other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synaptophysin Antibody

Western Blot: Synaptophysin Antibody [NB300-653]

Western Blot: Synaptophysin Antibody [NB300-653]

Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain dilution 1:100
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:00 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Rat brain-Synaptophysin.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653]

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Mouse Brain- Synaptophysin.
Synaptophysin Antibody

Western Blot: Synaptophysin Antibody [NB300-653] -

Extraction and characterization of synaptosomes.a Brief workflow of the current study. b Immunoblotting analysis of synaptic (SNAP25, synaptophysin and PSD95) and cytosolic (elF1a and PCNA) proteins in cytosolic fraction, synaptosomal fraction and leftover tissue debris of unaffected control postmortem brain tissues. c Densitometry analysis of synaptic and cytosolic proteins. Synaptic proteins levels (PSD95; P = 0.003), (SNAP25; P = 0.0061), (Synaptophysin; P = 0.026) were significantly higher in synaptosomes and cytosolic proteins (elF1a; P = 0.012) and (PCNA; P = 0.018) levels were significantly lower in synaptosomes relative to cytosol. d qRT-PCR analysis for mRNA fold change analysis of synaptic and cytosolic genes in cytosolic and synaptosomal fractions (n = 5). e TEM analysis of synapse assembly in synaptosomal fraction from unaffected control and AD patients’ postmortem brains (scale bar 500 nm magnification). Electron micrograph shows synapse components: Mt mitochondria, SV synaptic vesicles, PSD postsynaptic density, SC synaptic cleft. f Immunoblotting analysis of brain cells markers (Neuron-NeuN; Microglia-Iba1), excitatory synapse marker (VGLUT1) and inhibitory synapse marker (GABARA1) proteins in unaffected controls (n = 4) and AD (n = 4) synaptosomes. g Densitometry analysis of NeuN, Iba1, VGLUT1, and GABARA1 proteins in unaffected controls and AD synaptosomes. All blots are driven from the same experiment and were proceed parallelly (b, f). Values in the bar diagrams are mean +/- SEM and error bars are equivalent throughout the figure (c, d, g). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35941185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Synaptophysin Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:125

Western Blot

1:500-1:1000
Application Notes
Immunofluorescence reactivity reported in (PMID: 21368026). Immunoprecipitation reactivity reported in (PMID: 22970294). Synaptophysin antibody validated for IP, IHC-Frozen from verified customer reviews.

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-653 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptophysin

Synaptophysin is a 38 kDa synaptic vesicle associated protein. The pathology of synaptic components is investigated by measuring the expression of synaptophysin that is mainly concentrated in synaptic terminals. The expression of synaptophysin is a measure of dynamic plastic changes in nerve tissue. A decrease in synaptophysin and other synaptic vesicle associated proteins (Synaptotagmin, synaptosomal associated protein 25 kDa, SNAP-25, CLC ad GAP-43) generally means degeneration in the nerve fibers and an increased expression results in enhanced neuroplasticity and neuronal hypertrophy. In conditions involving generative changes in the nerve tissue, the expression of psynaptophysin is generally reduced, for example, in Schizophrenia an Alzhiemers disease. A decreased expression of synaptophysin in hippocampus of Schizoprenic patients has also been reported.

Alternate Names

SYP

Entrez Gene IDs

6855 (Human); 20977 (Mouse); 24804 (Rat)

Gene Symbol

SYP

UniProt

P08247

Additional Synaptophysin Products

Product Documents for Synaptophysin Antibody

Certificate of Analysis

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Product Specific Notices for Synaptophysin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Synaptophysin Antibody

Customer Reviews for Synaptophysin Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
0%
2 Stars
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1 Stars
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Customer Images

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  • Synaptophysin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Retina
    Species: Feline
    Verified Customer | Posted 09/15/2018
    Synaptophysin was immunolabelied shown as green. DAPI (blue). The image was captured using epifluorescent microscope.
    Synaptophysin Antibody NB300-653
  • Name: Brittany Weibley
    Application: Immunoprecipitation
    Sample Tested: Rat Retinal Tissue
    Species: Rat
    Verified Customer | Posted 08/16/2010

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