TACS Annexin V-FITC Apoptosis Detection Kit

R&D Systems | Catalog # 4830-250-K

R&D Systems
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Key Product Details

Features

Allows rapid, specific, and quantitative identification of apoptosis in individual cells when using flow cytometry.

Species

Multi-Species

Product Summary for TACS Annexin V-FITC Apoptosis Detection Kit

Why Use the TACS Annexin V-FITC Apoptosis Detection Kit?
Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine(PS) during an early apoptosis event where the PS becomes exposed at the cell surface.

The TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, allowing discrimination between apoptotic and necrotic (or late apoptotic) cells.

Scientific Data Images for TACS Annexin V-FITC Apoptosis Detection Kit

TACS Annexin V-FITC Apoptosis Detection Kit_4830-250-K

Detection of Apoptotic Dexamethasone-treated Thymocytes by Annexin V Staining.

Thymocytes were left untrated (left) or treated with 100 nM Dexamethasone for 15.5 hours (right) and then stained using Annexin V-FITC and propidium iodide provided in the TACS Annexin V-FITC Apoptosis Detection Kit (Catalog # 4830-250-K). The combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Analysis courtesy of Dr. C.M. Knudson, Howard Hughes Medical Institute, St. Louis, MO.

Kit Contents for TACS Annexin V-FITC Apoptosis Detection Kit

  • Annexin V FITC
  • 10X Binding Buffer
  • Propidium Iodide

Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Annexin V

Annexin V is a member of a calcium and phospholipid binding family of proteins with vascular anticoagulant activity. It is also known as PP4, PAP I, CPB-I, CaBP33, VACa, anchorin CII, lipocortin-V, endonexin II, and thromboplastin inhibitor.

Alternate Names

ANX5, ANXA5, ANXV

Additional Annexin V Products

Product Documents for TACS Annexin V-FITC Apoptosis Detection Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Related Research Areas

Coagulation

Citations for TACS Annexin V-FITC Apoptosis Detection Kit

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