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TACS Annexin V-FITC Apoptosis Detection Kit

4830-250-K 46 Citations 1 Review Datasheet / COA / SDS
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4830-01-K
4830-250-K
TACS Annexin V-FITC Apoptosis Detection Kit_4830-250-K
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Citations (46)
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TACS Annexin V-FITC Apoptosis Detection Kit Summary

Allows rapid, specific, and quantitative identification of apoptosis in individual cells when using flow cytometry.

Why Use the TACS Annexin V-FITC Apoptosis Detection Kit?

Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine(PS) during an early apoptosis event where the PS becomes exposed at the cell surface.

The TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, allowing discrimination between apoptotic and necrotic (or late apoptotic) cells.

Kit Contents

• Annexin V FITC
• 10X Binding Buffer
• Propidium Iodide

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Product Datasheet
COA
SDS

Data Example

Flow Cytometry TACS Annexin V-FITC Apoptosis Detection Kit_4830-250-K View Larger

Detection of Apoptotic Dexamethasone-treated Thymocytes by Annexin V Staining. Thymocytes were left untrated (left) or treated with 100 nM Dexamethasone for 15.5 hours (right) and then stained using Annexin V-FITC and propidium iodide provided in the TACS Annexin V-FITC Apoptosis Detection Kit (Catalog # 4830-250-K). The combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Analysis courtesy of Dr. C.M. Knudson, Howard Hughes Medical Institute, St. Louis, MO.

Citations for TACS Annexin V-FITC Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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  1. Au2phen and Auoxo6, Two Dinuclear Oxo-Bridged Gold(III) Compounds, Induce Apoptotic Signaling in Human Ovarian A2780 Cancer Cells
    Authors: G Gorini, F Magherini, T Fiaschi, L Massai, M Becatti, A Modesti, L Messori, T Gamberi
    Biomedicines, 2021;9(8):.  2021
  2. The Effects of Muscle Cell Aging on Myogenesis
    Authors: A Moustogian, A Philippou, O Taso, E Zevolis, M Pappa, A Chatzigeor, M Koutsilier
    International Journal of Molecular Sciences, 2021;22(7):.  2021
  3. Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition
    Authors: AM Waters, TO Khatib, B Papke, CM Goodwin, GA Hobbs, JN Diehl, R Yang, AC Edwards, KH Walsh, R Sulahian, JM McFarland, KS Kapner, TSK Gilbert, CA Stalnecker, S Javaid, A Barkovskay, KR Grover, PS Hibshman, DR Blake, A Schaefer, KM Nowak, JE Klomp, TK Hayes, M Kassner, N Tang, O Tanaseichu, K Chen, Y Zhou, M Kalkat, LE Herring, LM Graves, LZ Penn, HH Yin, AJ Aguirre, WC Hahn, AD Cox, CJ Der
    Cell Reports, 2021;35(13):109291.  2021
  4. In vivo fermentation production of humanized noncoding RNAs carrying payload miRNAs for targeted anticancer therapy
    Authors: PC Li, MJ Tu, PY Ho, N Batra, MML Tran, JX Qiu, T Wun, PN Lara, X Hu, AX Yu, AM Yu
    Theranostics, 2021;11(10):4858-4871.  2021
  5. Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
    Authors: JY Lee, DJ Min, W Kim, BH Bin, K Kim, EG Cho
    Scientific Reports, 2021;11(1):2465.  2021
  6. Polymer Coated Oncolytic Adenovirus to Selectively Target Hepatocellular Carcinoma Cells
    Authors: M Garofalo, F Bellato, S Magliocca, A Malfanti, L Kuryk, B Rinner, S Negro, S Salmaso, P Caliceti, F Mastrotto
    Pharmaceutics, 2021;13(7):.  2021
  7. Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells
    Authors: S Funakoshi, I Fernandes, O Mastikhina, D Wilkinson, T Tran, W Dhahri, A Mazine, D Yang, B Burnett, J Lee, S Protze, GD Bader, SS Nunes, M Laflamme, G Keller
    Nature Communications, 2021;12(1):3155.  2021
  8. LDHA induces EMT gene transcription and regulates autophagy to promote the metastasis and tumorigenesis of papillary thyroid carcinoma
    Authors: X Hou, X Shi, W Zhang, D Li, L Hu, J Yang, J Zhao, S Wei, X Wei, X Ruan, X Zheng, M Gao
    Cell Death & Disease, 2021;12(4):347.  2021
  9. Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal Cancer Stem Cells in vitro and in vivo
    Authors: WS Koom, S Sai, M Suzuki, A Fujimori, S Yamada, H Tsujii
    OncoTargets and Therapy, 2020;13(0):12625-12635.  2020
  10. Mitochondrial dysfunction and DNA damage accompany enhanced levels of formaldehyde in cultured primary human fibroblasts
    Authors: CA Nadalutti, DF Stefanick, ML Zhao, JK Horton, R Prasad, AM Brooks, JD Griffith, SH Wilson
    Sci Rep, 2020;10(1):5575.  2020
  11. IGF2 autocrine-mediated IGF1R activation is a clinically relevant mechanism of osimertinib resistance in lung cancer
    Authors: T Manabe, H Yasuda, H Terai, H Kagiwada, J Hamamoto, T Ebisudani, K Kobayashi, K Masuzawa, S Ikemura, I Kawada, Y Hayashi, K Fukui, K Horimoto, K Fukunaga, K Soejima
    Mol. Cancer Res., 2020;0(0):.  2020
  12. Apatinib ameliorates doxorubicin-induced migration and cancer stemness of osteosarcoma cells by inhibiting Sox2 via STAT3 signalling
    Authors: ZC Tian, JQ Wang, H Ge
    J Orthop Translat, 2020;22(0):132-141.  2020
  13. Indole-6-Carboxaldehyde Isolated from Sargassum thunbergii (Mertens) Kuntze Prevents Oxidative Stress-Induced Cellular Damage in V79-4 Chinese Hamster Lung Fibroblasts through the Activation of the Nrf2/HO-1 Signaling Pathway
    Authors: SO Kim, YH Choi
    Cell Physiol Biochem, 2020;54(5):959-974.  2020
  14. Inhibition of Heparanase Expression Results in Suppression of Invasion, Migration and Adhesion Abilities of Bladder Cancer Cells
    Authors: Y Tatsumi, M Miyake, K Shimada, T Fujii, S Hori, Y Morizawa, Y Nakai, S Anai, N Tanaka, N Konishi, K Fujimoto
    Int J Mol Sci, 2020;21(11):.  2020
  15. Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes
    Authors: G Ambreen, L Duse, I Tariq, U Ali, S Ali, SR Pinnapired, M Bette, U Bakowsky, R Mandic
    Cancers (Basel), 2020;12(11):.  2020
  16. Evaluation of immunomodulatory effects of Boswellia sacra essential oil on T-cells and dendritic cells
    Authors: AM Aldahlawi, AT Alzahrani, MF Elshal
    BMC Complement Med Ther, 2020;20(1):352.  2020
  17. Exosomal lncRNA H19 promotes the progression of hepatocellular carcinoma treated with Propofol via miR-520a-3p/LIMK1 axis
    Authors: D Wang, N Xing, T Yang, J Liu, H Zhao, J He, Y Ai, J Yang
    Cancer Med, 2020;0(0):.  2020
  18. Protein tyrosine phosphatase 1B as a therapeutic target for Graves' orbitopathy in an in vitro model
    Authors: HJ Byeon, JY Kim, J Ko, EJ Lee, K Don, JS Yoon
    PLoS ONE, 2020;15(8):e0237015.  2020
  19. Hemistepsin A protects human keratinocytes against hydrogen peroxide-induced oxidative stress through activation of the Nrf2/HO-1 signaling pathway
    Authors: C Park, H Lee, JS Noh, CY Jin, GY Kim, JW Hyun, SH Leem, YH Choi
    Arch. Biochem. Biophys., 2020;691(0):108512.  2020
  20. Oxidative Stress Triggers Selective tRNA Retrograde Transport in Human Cells during the Integrated Stress Response
    Authors: H Schwenzer, F Jühling, A Chu, LJ Pallett, TF Baumert, M Maini, A Fassati
    Cell Rep, 2019;26(12):3416-3428.e5.  2019
  21. Efferocytosis of apoptotic alveolar epithelial cells is sufficient to initiate lung fibrosis
    Authors: KK Kim, MR Dotson, M Agarwal, J Yang, PB Bradley, N Subbotina, JJ Osterholze, TH Sisson
    Cell Death Dis, 2018;9(11):1056.  2018
  22. EBV induces persistent NF-?B activation and contributes to survival of EBV-positive neoplastic T- or NK-cells
    Authors: H Takada, KI Imadome, H Shibayama, M Yoshimori, L Wang, Y Saitoh, S Uota, S Yamaoka, T Koyama, N Shimizu, K Yamamoto, S Fujiwara, O Miura, A Arai
    PLoS ONE, 2017;12(3):e0174136.  2017
  23. Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis
    Authors: MJ Mitchell, J Webster, A Chung, PP Guimarães, OF Khan, R Langer
    Nat Commun, 2017;8(0):14179.  2017
  24. FGF-23 is a negative regulator of prenatal and postnatal erythropoiesis.
    Authors: Coe, Lindsay, Madathil, Sangeeth, Casu, Carla, Lanske, Beate, Rivella, Stefano, Sitara, Despina
    J Biol Chem, 2014;289(14):9795-810.  2014
  25. Plasma membrane Pdia3 and VDR interact to elicit rapid responses to 1alpha,25(OH)(2)D(3).
    Authors: Chen J, Doroudi M, Cheung J, Grozier A, Schwartz Z, Boyan B
    Cell Signal, 2013;25(12):2362-73.  2013
  26. PCK3145 inhibits proliferation and induces apoptosis in breast and colon cancer cells.
    Authors: Kostopoulos A, Papageorgiou E, Koutsilieris M, Sivolapenko G
    Anticancer Res, 0;35(3):1377-84.  0
  27. Tyrosine-phosphorylated caveolin-1 (Tyr-14) increases sensitivity to paclitaxel by inhibiting BCL2 and BCLxL proteins via c-Jun N-terminal kinase (JNK).
    Authors: Shajahan A, Dobbin Z, Hickman F, Dakshanamurthy S, Clarke R
    J Biol Chem, 0;287(21):17682-92.  0
  28. The novel BCR-ABL and FLT3 inhibitor ponatinib is a potent inhibitor of the MDR-associated ATP-binding cassette transporter ABCG2.
    Authors: Sen R, Natarajan K, Bhullar J, Shukla S, Fang H, Cai L, Chen Z, Ambudkar S, Baer M
    Mol Cancer Ther, 0;11(9):2033-44.  0
  29. MicroRNA-148a reduces tumorigenesis and increases TRAIL-induced apoptosis in NSCLC.
    Authors: Joshi P, Jeon Y, Lagana A, Middleton J, Secchiero P, Garofalo M, Croce C
    Proc Natl Acad Sci U S A, 0;112(28):8650-5.  0
  30. Reovirus uses multiple endocytic pathways for cell entry.
    Authors: Schulz W, Haj A, Schiff L
    J Virol, 0;86(23):12665-75.  0
  31. Mechanisms of cyclic AMP/protein kinase A- and glucocorticoid-mediated apoptosis using S49 lymphoma cells as a model system.
    Authors: Keshwani M, Kanter J, Ma Y, Wilderman A, Darshi M, Insel P, Taylor S
    Proc Natl Acad Sci U S A, 0;112(41):12681-6.  0
  32. CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure.
    Authors: Kuse Y, Tsuruma K, Kanno Y, Shimazawa M, Hara H
    Front Pharmacol, 0;8(0):207.  0
  33. IFN-gamma-independent intraocular tumor rejection is mediated by a macrophage-dependent process that leaves the eye intact.
    Authors: Coursey T, Chen P, Niederkorn J
    J Leukoc Biol, 0;92(5):939-50.  0
  34. CBS9106-induced CRM1 degradation is mediated by cullin ring ligase activity and the neddylation pathway.
    Authors: Saito N, Sakakibara K, Sato T, Friedman J, Kufe D, VonHoff D, Kawabe T
    Mol Cancer Ther, 0;13(12):3013-23.  0
  35. A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications.
    Authors: Chen Q, Wang W, Zeng S, Urayama S, Yu A
    Nucleic Acids Res, 0;43(7):3857-69.  0
  36. Pharmacological Inhibition of the Histone Lysine Demethylase KDM1A Suppresses the Growth of Multiple Acute Myeloid Leukemia Subtypes.
    Authors: McGrath J, Williamson K, Balasubramanian S, Odate S, Arora S, Hatton C, Edwards T, O'Brien T, Magnuson S, Stokoe D, Daniels D, Bryant B, Trojer P
    Cancer Res, 0;76(7):1975-88.  0
  37. Mitochondrial DNA-depleted A549 cells are resistant to bleomycin.
    Authors: Brar S, Meyer J, Bortner C, Van Houten B, Martin W
    Am J Physiol Lung Cell Mol Physiol, 0;303(5):L413-24.  0
  38. Dichotomous roles for externalized cardiolipin in extracellular signaling: Promotion of phagocytosis and attenuation of innate immunity.
    Authors: Balasubramanian K, Maeda A, Lee J, Mohammadyani D, Dar H, Jiang J, St Croix C, Watkins S, Tyurin V, Tyurina Y, Kloditz K, Polimova A, Kapralova V, Xiong Z, Ray P, Klein-Seetharaman J, Mallampalli R, Bayir H, Fadeel B, Kagan V
    Sci Signal, 0;8(395):ra95.  0
  39. Kruppel-like factor 9 (KLF9) prevents colorectal cancer through inhibition of interferon-related signaling.
    Authors: Brown A, Simmen R, Raj V, Van T, MacLeod S, Simmen F
    Carcinogenesis, 0;36(9):946-55.  0
  40. Poly(ADP-ribose) polymerase-1 inhibition by arsenite promotes the survival of cells with unrepaired DNA lesions induced by UV exposure.
    Authors: Qin X, Liu W, Li Y, Sun X, Hai C, Hudson L, Liu K
    Toxicol Sci, 0;127(1):120-9.  0
  41. An LRP16-containing preassembly complex contributes to NF-kappaB activation induced by DNA double-strand breaks.
    Authors: Wu Z, Wang C, Bai M, Li X, Mei Q, Li X, Wang Y, Fu X, Luo G, Han W
    Nucleic Acids Res, 0;43(6):3167-79.  0
  42. Endothelial Cells Inhibit the Angiotensin II Induced Phenotypic Modulation of Rat Vascular Adventitial Fibroblasts.
    Authors: Xu J, Chang N, Li T, Jiang R, Sun X, He Y, Jiang J
    J Cell Biochem, 0;118(7):1921-1927.  0
  43. Targeting acute myeloid leukemia with a small molecule inhibitor of the Myb/p300 interaction.
    Authors: Uttarkar S, Dasse E, Coulibaly A, Steinmann S, Jakobs A, Schomburg C, Trentmann A, Jose J, Schlenke P, Berdel W, Schmidt T, Muller-Tidow C, Frampton J, Klempnauer K
    Blood, 0;127(9):1173-82.  0
  44. Staphylococcus aureus Inhibits Neutrophil-derived IL-8 to Promote Cell Death.
    Authors: Zurek O, Pallister K, Voyich J
    J Infect Dis, 0;212(6):934-8.  0
  45. A quantitative assay reveals ligand specificity of the DNA scaffold repair protein XRCC1 and efficient disassembly of complexes of XRCC1 and the poly(ADP-ribose) polymerase 1 by poly(ADP-ribose) glycohydrolase.
    Authors: Kim I, Stegeman R, Brosey C, Ellenberger T
    J Biol Chem, 0;290(6):3775-83.  0
  46. Naphthol AS-E Phosphate Inhibits the Activity of the Transcription Factor Myb by Blocking the Interaction with the KIX Domain of the Coactivator p300.
    Authors: Uttarkar S, Dukare S, Bopp B, Goblirsch M, Jose J, Klempnauer K
    Mol Cancer Ther, 0;14(6):1276-85.  0

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TACS Annexin V-FITC Apoptosis Detection Kit
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