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TACS Annexin V-FITC Apoptosis Detection Kit

Catalog #: 4830-250-K
76 Citations 1 Review Datasheet / COA / SDS
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4830-01-K
4830-250-K
TACS Annexin V-FITC Apoptosis Detection Kit_4830-250-K
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TACS Annexin V-FITC Apoptosis Detection Kit Summary

Allows rapid, specific, and quantitative identification of apoptosis in individual cells when using flow cytometry.

Why Use the TACS Annexin V-FITC Apoptosis Detection Kit?

Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine(PS) during an early apoptosis event where the PS becomes exposed at the cell surface.

The TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, allowing discrimination between apoptotic and necrotic (or late apoptotic) cells.

Kit Contents

• Annexin V FITC
• 10X Binding Buffer
• Propidium Iodide

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

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Scientific Data

Flow Cytometry TACS Annexin V-FITC Apoptosis Detection Kit_4830-250-K View Larger

Detection of Apoptotic Dexamethasone-treated Thymocytes by Annexin V Staining. Thymocytes were left untrated (left) or treated with 100 nM Dexamethasone for 15.5 hours (right) and then stained using Annexin V-FITC and propidium iodide provided in the TACS Annexin V-FITC Apoptosis Detection Kit (Catalog # 4830-250-K). The combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Analysis courtesy of Dr. C.M. Knudson, Howard Hughes Medical Institute, St. Louis, MO.

Citations for TACS Annexin V-FITC Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

76 Citations: Showing 1 - 10
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  1. NRF2 Regulates Viability, Proliferation, Resistance to Oxidative Stress, and Differentiation of Murine Myoblasts and Muscle Satellite Cells
    Authors: I Bronisz-Bu, M Kozakowska, K Pietraszek, M Madej, A Józkowicz, A ?oboda, J Dulak
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    Journal of pharmacological sciences, 2022;148(2):204-213.  2022
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    Infectious agents and cancer, 2022;17(1):4.  2022
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  8. DNA damage alters EGFR signaling and reprograms cellular response via Mre-11
    Authors: Y Volman, R Hefetz, E Galun, J Rachmilewi
    Scientific Reports, 2022;12(1):5760.  2022
  9. Transcriptomics-Based Investigation of Molecular Mechanisms Underlying Apoptosis Induced by ZnO Nanoparticles in Human Diffuse Large B-Cell Lymphoma
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    International Journal of Nanomedicine, 2022;17(0):2261-2281.  2022
  10. Magnesium impairs Candida albicans immune evasion by reduced hyphal damage, enhanced beta-glucan exposure and altered vacuole homeostasis
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  11. Berberine regulates PADI4-related macrophage function to prevent lung cancer
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    Oncogene, 2022;110(0):108965.  2022
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    Authors: J Shi, X Jin, Y Wang, T Zhu, D Zhang, Q Li, X Zhong, Y Deng, J Shen, X Fan
    Oncogenesis, 2022;11(1):37.  2022
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    Authors: MK Ko, JI Woo, JM Gonzalez, G Kim, L Sakai, J Peti-Peter, JA Kelber, YK Hong, JC Tan
    Oncogene, 2022;12(1):10623.  2022
  14. AP39, a Mitochondrial-Targeted H2S Donor, Improves Porcine Islet Survival in Culture
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    Journal of Clinical Medicine, 2022;11(18):.  2022
  15. Effects of bone morphogenetic protein 4 on TGF-beta1-induced cell proliferation, apoptosis, activation and differentiation in mouse lung fibroblasts via ERK/p38 MAPK signaling pathway
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    PeerJ, 2022;10(0):e13775.  2022
  16. Inhibition of lipid metabolism exerts antitumor effects on rhabdomyosarcoma
    Authors: S Miyagaki, K Kikuchi, J Mori, GD Lopaschuk, T Iehara, H Hosoi
    Cancer Medicine, 2021;10(18):6442-6455.  2021
  17. Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
    Authors: JY Lee, DJ Min, W Kim, BH Bin, K Kim, EG Cho
    Scientific Reports, 2021;11(1):2465.  2021
  18. The Effects of Muscle Cell Aging on Myogenesis
    Authors: A Moustogian, A Philippou, O Taso, E Zevolis, M Pappa, A Chatzigeor, M Koutsilier
    International Journal of Molecular Sciences, 2021;22(7):.  2021
  19. LDHA induces EMT gene transcription and regulates autophagy to promote the metastasis and tumorigenesis of papillary thyroid carcinoma
    Authors: X Hou, X Shi, W Zhang, D Li, L Hu, J Yang, J Zhao, S Wei, X Wei, X Ruan, X Zheng, M Gao
    Cell Death & Disease, 2021;12(4):347.  2021
  20. Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells
    Authors: S Funakoshi, I Fernandes, O Mastikhina, D Wilkinson, T Tran, W Dhahri, A Mazine, D Yang, B Burnett, J Lee, S Protze, GD Bader, SS Nunes, M Laflamme, G Keller
    Nature Communications, 2021;12(1):3155.  2021
  21. Au2phen and Auoxo6, Two Dinuclear Oxo-Bridged Gold(III) Compounds, Induce Apoptotic Signaling in Human Ovarian A2780 Cancer Cells
    Authors: G Gorini, F Magherini, T Fiaschi, L Massai, M Becatti, A Modesti, L Messori, T Gamberi
    Biomedicines, 2021;9(8):.  2021
  22. Rosiglitazone Suppresses Renal Crystal Deposition by Ameliorating Tubular Injury Resulted from Oxidative Stress and Inflammatory Response via Promoting the Nrf2/HO-1 Pathway and Shifting Macrophage Polarization
    Authors: H Lu, X Sun, M Jia, F Sun, J Zhu, X Chen, K Chen, K Jiang
    Oxidative Medicine and Cellular Longevity, 2021;2021(0):5527137.  2021
  23. X-ray repair cross-complementing protein 1 (XRCC1) loss promotes beta-lapachone -induced apoptosis in pancreatic cancer cells
    Authors: Y Zheng, H Zhang, Y Guo, Y Chen, H Chen, Y Liu
    BMC Cancer, 2021;21(1):1234.  2021
  24. Morc2a p.S87L mutant mice develop peripheral and central neuropathies associated with neuronal DNA damage and apoptosis
    Authors: GS Lee, G Kwak, JH Bae, JP Han, SH Nam, JH Lee, S Song, GD Kim, TS Park, YK Choi, BO Choi, SC Yeom
    Disease Models & Mechanisms, 2021;14(10):.  2021
  25. Synergistic apoptotic effect of miR-183-5p and Polo-Like kinase 1 inhibitor NMS-P937 in breast cancer cells
    Authors: M Kudo, N Zalles, R Distefano, G Nigita, D Veneziano, P Gasparini, CM Croce
    Cell Death and Differentiation, 2021;0(0):.  2021
  26. A novel miRNA-762/NFIX pathway modulates LPS-induced acute lung injury
    Authors: XL Zhang, J An, YZ Deng, XZ Fang, CY Xu, XF Liu, ZH Bai, G Zhang, MY Cui
    International immunopharmacology, 2021;100(0):108066.  2021
  27. MLK4 regulates DNA damage response and promotes triple-negative breast cancer chemoresistance
    Authors: D Mehlich, M ?omiak, A Sobiborowi, A Mazan, D Dymerska, ?M Szewczyk, A Mehlich, A Borowiec, MK Pre?owska, A Gorczy?ski, P Jab?o?ski, E I?ycka-?wi, D Nowis, AA Marusiak
    Cell Death & Disease, 2021;12(12):1111.  2021
  28. CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment
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    Cell Reports, 2021;37(9):110060.  2021
  29. Polymer Coated Oncolytic Adenovirus to Selectively Target Hepatocellular Carcinoma Cells
    Authors: M Garofalo, F Bellato, S Magliocca, A Malfanti, L Kuryk, B Rinner, S Negro, S Salmaso, P Caliceti, F Mastrotto
    Pharmaceutics, 2021;13(7):.  2021
  30. A distinct metabolic response characterizes sensitivity to EZH2 inhibition in multiple myeloma
    Authors: P Nylund, A Atienza Pá, J Haglöf, E De Bruyne, E Menu, B Garrido-Za, A Ma, J Jin, F Öberg, K Vanderkerk, A Kalushkova, H Jernberg-W
    Cell Death & Disease, 2021;12(2):167.  2021
  31. Synthesis and Biological Evaluation of Novel Isoxazole-Amide Analogues as Anticancer and Antioxidant Agents
    Authors: AM Eid, M Hawash, J Amer, A Jarrar, S Qadri, I Alnimer, A Sharaf, R Zalmoot, O Hammoudie, S Hameedi, A Mousa
    BioMed Research International, 2021;2021(0):6633297.  2021
  32. In vivo fermentation production of humanized noncoding RNAs carrying payload miRNAs for targeted anticancer therapy
    Authors: PC Li, MJ Tu, PY Ho, N Batra, MML Tran, JX Qiu, T Wun, PN Lara, X Hu, AX Yu, AM Yu
    Theranostics, 2021;11(10):4858-4871.  2021
  33. Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition
    Authors: AM Waters, TO Khatib, B Papke, CM Goodwin, GA Hobbs, JN Diehl, R Yang, AC Edwards, KH Walsh, R Sulahian, JM McFarland, KS Kapner, TSK Gilbert, CA Stalnecker, S Javaid, A Barkovskay, KR Grover, PS Hibshman, DR Blake, A Schaefer, KM Nowak, JE Klomp, TK Hayes, M Kassner, N Tang, O Tanaseichu, K Chen, Y Zhou, M Kalkat, LE Herring, LM Graves, LZ Penn, HH Yin, AJ Aguirre, WC Hahn, AD Cox, CJ Der
    Cell Reports, 2021;35(13):109291.  2021
  34. Exosomal lncRNA H19 promotes the progression of hepatocellular carcinoma treated with Propofol via miR-520a-3p/LIMK1 axis
    Authors: D Wang, N Xing, T Yang, J Liu, H Zhao, J He, Y Ai, J Yang
    Cancer Med, 2020;0(0):.  2020
  35. Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal Cancer Stem Cells in vitro and in vivo
    Authors: WS Koom, S Sai, M Suzuki, A Fujimori, S Yamada, H Tsujii
    OncoTargets and Therapy, 2020;13(0):12625-12635.  2020
  36. Evaluation of immunomodulatory effects of Boswellia sacra essential oil on T-cells and dendritic cells
    Authors: AM Aldahlawi, AT Alzahrani, MF Elshal
    BMC Complement Med Ther, 2020;20(1):352.  2020
  37. Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes
    Authors: G Ambreen, L Duse, I Tariq, U Ali, S Ali, SR Pinnapired, M Bette, U Bakowsky, R Mandic
    Cancers (Basel), 2020;12(11):.  2020
  38. Protein tyrosine phosphatase 1B as a therapeutic target for Graves' orbitopathy in an in vitro model
    Authors: HJ Byeon, JY Kim, J Ko, EJ Lee, K Don, JS Yoon
    PLoS ONE, 2020;15(8):e0237015.  2020
  39. Protein tyrosine phosphatase 1B as a therapeutic target for Graves' orbitopathy in an in vitro model
    Authors: HJ Byeon, JY Kim, J Ko, EJ Lee, K Don, JS Yoon
    PLoS ONE, 2020;15(8):e0237015.  2020
  40. Hemistepsin A protects human keratinocytes against hydrogen peroxide-induced oxidative stress through activation of the Nrf2/HO-1 signaling pathway
    Authors: C Park, H Lee, JS Noh, CY Jin, GY Kim, JW Hyun, SH Leem, YH Choi
    Arch. Biochem. Biophys., 2020;691(0):108512.  2020
  41. Mitochondrial dysfunction and DNA damage accompany enhanced levels of formaldehyde in cultured primary human fibroblasts
    Authors: CA Nadalutti, DF Stefanick, ML Zhao, JK Horton, R Prasad, AM Brooks, JD Griffith, SH Wilson
    Sci Rep, 2020;10(1):5575.  2020
  42. Inhibition of Heparanase Expression Results in Suppression of Invasion, Migration and Adhesion Abilities of Bladder Cancer Cells
    Authors: Y Tatsumi, M Miyake, K Shimada, T Fujii, S Hori, Y Morizawa, Y Nakai, S Anai, N Tanaka, N Konishi, K Fujimoto
    Int J Mol Sci, 2020;21(11):.  2020
  43. Apatinib ameliorates doxorubicin-induced migration and cancer stemness of osteosarcoma cells by inhibiting Sox2 via STAT3 signalling
    Authors: ZC Tian, JQ Wang, H Ge
    J Orthop Translat, 2020;22(0):132-141.  2020
  44. IGF2 autocrine-mediated IGF1R activation is a clinically relevant mechanism of osimertinib resistance in lung cancer
    Authors: T Manabe, H Yasuda, H Terai, H Kagiwada, J Hamamoto, T Ebisudani, K Kobayashi, K Masuzawa, S Ikemura, I Kawada, Y Hayashi, K Fukui, K Horimoto, K Fukunaga, K Soejima
    Mol. Cancer Res., 2020;0(0):.  2020
  45. Indole-6-Carboxaldehyde Isolated from Sargassum thunbergii (Mertens) Kuntze Prevents Oxidative Stress-Induced Cellular Damage in V79-4 Chinese Hamster Lung Fibroblasts through the Activation of the Nrf2/HO-1 Signaling Pathway
    Authors: SO Kim, YH Choi
    Cell Physiol Biochem, 2020;54(5):959-974.  2020
  46. Exosomal lncRNA H19 promotes the progression of hepatocellular carcinoma treated with Propofol via miR-520a-3p/LIMK1 axis
    Authors: D Wang, N Xing, T Yang, J Liu, H Zhao, J He, Y Ai, J Yang
    Cancer Med, 2020;0(0):.  2020
  47. Low-Dose Vertical Inhibition of the RAF-MEK-ERK Cascade Causes Apoptotic Death of KRAS Mutant Cancers
    Authors: I Ozkan-Dagl, JN Diehl, SD George, A Schaefer, B Papke, K Klotz-Noac, AM Waters, CM Goodwin, P Gautam, M Pierobon, S Peng, TSK Gilbert, KH Lin, O Dagliyan, K Wennerberg, EF Petricoin, NL Tran, SV Bhagwat, RV Tiu, SB Peng, LE Herring, LM Graves, C Sers, KC Wood, AD Cox, CJ Der
    Cell Rep, 2020;31(11):107764.  2020
  48. Vulnerability of drug-resistant EML4-ALK rearranged lung cancer to transcriptional inhibition
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  49. Anticancer effect of fucoidan on cell proliferation, cell cycle progression, genetic damage and apoptotic cell death in HepG2 cancer cells
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  51. Efferocytosis of apoptotic alveolar epithelial cells is sufficient to initiate lung fibrosis
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  52. Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis
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TACS Annexin V-FITC Apoptosis Detection Kit
By Anonymous on 02/24/2021
TACS Annexin V-FITC Apoptosis Detection Kit 4830-250-K