TACS Annexin V-FITC Apoptosis Detection Kit Summary
Allows rapid, specific, and quantitative identification of apoptosis in individual cells when using flow cytometry.Why Use the TACS Annexin V-FITC Apoptosis Detection Kit?
Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine(PS) during an early apoptosis event where the PS becomes exposed at the cell surface.
The TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, allowing discrimination between apoptotic and necrotic (or late apoptotic) cells.
Kit Contents
• Annexin V FITC
• 10X Binding Buffer
• Propidium Iodide
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Scientific Data

Detection of Apoptotic Dexamethasone-treated Thymocytes by Annexin V Staining. Thymocytes were left untrated (left) or treated with 100 nM Dexamethasone for 15.5 hours (right) and then stained using Annexin V-FITC and propidium iodide provided in the TACS Annexin V-FITC Apoptosis Detection Kit (Catalog # 4830-250-K). The combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Analysis courtesy of Dr. C.M. Knudson, Howard Hughes Medical Institute, St. Louis, MO.
Citations for TACS Annexin V-FITC Apoptosis Detection Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes
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Oxidative Medicine and Cellular Longevity, 2021;2021(0):5527137. 2021-01-01
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Authors: P Nylund, A Atienza Pá, J Haglöf, E De Bruyne, E Menu, B Garrido-Za, A Ma, J Jin, F Öberg, K Vanderkerk, A Kalushkova, H Jernberg-W
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International immunopharmacology, 2021;100(0):108066. 2021-01-01
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MLK4 regulates DNA damage response and promotes triple-negative breast cancer chemoresistance
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Cell Death & Disease, 2021;12(12):1111. 2021-01-01
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CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment
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Cell Reports, 2021;37(9):110060. 2021-01-01
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Polymer Coated Oncolytic Adenovirus to Selectively Target Hepatocellular Carcinoma Cells
Authors: M Garofalo, F Bellato, S Magliocca, A Malfanti, L Kuryk, B Rinner, S Negro, S Salmaso, P Caliceti, F Mastrotto
Pharmaceutics, 2021;13(7):. 2021-01-01
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Authors: AM Eid, M Hawash, J Amer, A Jarrar, S Qadri, I Alnimer, A Sharaf, R Zalmoot, O Hammoudie, S Hameedi, A Mousa
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Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
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Apatinib ameliorates doxorubicin-induced migration and cancer stemness of osteosarcoma cells by inhibiting Sox2 via STAT3 signalling
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Evaluation of immunomodulatory effects of Boswellia sacra essential oil on T-cells and dendritic cells
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Vulnerability of drug-resistant EML4-ALK rearranged lung cancer to transcriptional inhibition
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IGF2 autocrine-mediated IGF1R activation is a clinically relevant mechanism of osimertinib resistance in lung cancer
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Low-Dose Vertical Inhibition of the RAF-MEK-ERK Cascade Causes Apoptotic Death of KRAS Mutant Cancers
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Indole-6-Carboxaldehyde Isolated from Sargassum thunbergii (Mertens) Kuntze Prevents Oxidative Stress-Induced Cellular Damage in V79-4 Chinese Hamster Lung Fibroblasts through the Activation of the Nrf2/HO-1 Signaling Pathway
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Cell Physiol Biochem, 2020;54(5):959-974. 2020-01-01
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Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal Cancer Stem Cells in vitro and in vivo
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Hemistepsin A protects human keratinocytes against hydrogen peroxide-induced oxidative stress through activation of the Nrf2/HO-1 signaling pathway
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Mitochondrial dysfunction and DNA damage accompany enhanced levels of formaldehyde in cultured primary human fibroblasts
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Sci Rep, 2020;10(1):5575. 2020-01-01
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Anticancer effect of fucoidan on cell proliferation, cell cycle progression, genetic damage and apoptotic cell death in HepG2 cancer cells
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Oxidative Stress Triggers Selective tRNA Retrograde Transport in Human Cells during the Integrated Stress Response
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Efferocytosis of apoptotic alveolar epithelial cells is sufficient to initiate lung fibrosis
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Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis
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