TAF7 Antibody (2C5) - Azide and BSA Free
Novus Biologicals | Catalog # H00006879-M01
![Western Blot: TAF7 Antibody (2C5) [H00006879-M01]](https://resources.rndsystems.com/images/products/TAF7-Antibody-2C5-Western-Blot-H00006879-M01-img0016.jpg "Western Blot: TAF7 Antibody (2C5) [H00006879-M01]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2C5
Format
Azide and BSA Free
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Product Specifications
Immunogen
TAF7 (AAH32737, 130 a.a. ~ 224 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FIWNHGITLPLKNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTRWEIIAEDETKEAENQGLDISSPGMSGHRQGHDSLEHDELREIFN
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:31914128).
Specificity
TAF7 - TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for TAF7 Antibody (2C5) - Azide and BSA Free
Western Blot: TAF7 Antibody (2C5) [H00006879-M01]
Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - TAF7 monoclonal antibody (M01), clone 2C5 Analysis of TAF7 expression in MCF-7.![Immunocytochemistry/ Immunofluorescence: TAF7 Antibody (2C5) [H00006879-M01]](https://resources.rndsystems.com/images/products/TAF7-Antibody-2C5-Immunocytochemistry-Immunofluorescence-H00006879-M01-img0009.jpg "Immunocytochemistry/ Immunofluorescence: TAF7 Antibody (2C5) [H00006879-M01]")
Immunocytochemistry/ Immunofluorescence: TAF7 Antibody (2C5) [H00006879-M01]
Immunocytochemistry/Immunofluorescence: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on HeLa cell. Antibody concentration 10 ug/ml.![Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01]](https://resources.rndsystems.com/images/products/TAF7-Antibody-2C5-Immunohistochemistry-Paraffin-H00006879-M01-img0013.jpg "Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01]")
Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01]
Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.![Western Blot: TAF7 Antibody (2C5) [H00006879-M01]](https://resources.rndsystems.com/images/products/TAF7-Antibody-2C5-Western-Blot-H00006879-M01-img0014.jpg "Western Blot: TAF7 Antibody (2C5) [H00006879-M01]")
Western Blot: TAF7 Antibody (2C5) [H00006879-M01]
Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi ( Cat # H00006879-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TAF7 monoclonal antibody (M01), clone 2C5 (Cat # H00006879-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: TAF7 Antibody (2C5) [H00006879-M01]](https://resources.rndsystems.com/images/products/TAF7-Antibody-2C5-Western-Blot-H00006879-M01-img0017.jpg "Western Blot: TAF7 Antibody (2C5) [H00006879-M01]")
Western Blot: TAF7 Antibody (2C5) [H00006879-M01]
Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of TAF7 expression in transfected 293T cell line by TAF7 monoclonal antibody (M01), clone 2C5.Lane 1: TAF7 transfected lysate(40.3 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01]](https://resources.rndsystems.com/images/products/TAF7-Antibody-2C5-Immunohistochemistry-Paraffin-H00006879-M01-img0011.jpg "Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01]")
Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01]
Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.![ELISA: TAF7 Antibody (2C5) [H00006879-M01]](https://resources.rndsystems.com/images/products/TAF7-Antibody-2C5-Sandwich-ELISA-H00006879-M01-img0015.jpg "ELISA: TAF7 Antibody (2C5) [H00006879-M01]")
ELISA: TAF7 Antibody (2C5) [H00006879-M01]
ELISA: TAF7 Antibody (2C5) [H00006879-M01] - Detection limit for recombinant GST tagged TAF7 is approximately 0.3ng/ml as a capture antibody.Applications for TAF7 Antibody (2C5) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TAF7
Alternate Names
TAF(II)55, TAF2FTBP-associated factor F, TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa, TAFII-55, TAFII55RNA polymerase II TBP-associated factor subunit F, TATA box binding protein (TBP)-associated factor, RNA polymerase II, F, 55kD, transcription factor IID subunit TAFII55, Transcription initiation factor TFIID 55 kDa subunit, transcription initiation factor TFIID subunit 7, transcription initiation factor TFIID, 55 kDa subunit
Entrez Gene IDs
6879 (Human)
Gene Symbol
TAF7
OMIM
600573 (Human)
UniProt
Additional TAF7 Products
Product Documents for TAF7 Antibody (2C5) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TAF7 Antibody (2C5) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for TAF7 Antibody (2C5) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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