Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-45158
Recombinant Monoclonal Antibody
![Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free](https://beta-resources.rndsystems.com/images/products/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-western-blot-152025102720.jpg "Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] -")
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Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2498
Format
Azide and BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr181 of human Tau. The exact sequence is proprietary.
Modification
p Thr181
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free (NBP3-45158) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free
Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] -
Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free](https://beta-resources.rndsystems.com/images/products/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-western-blot-152025103434..jpg "Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] -")
Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] -
Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free](https://beta-resources.rndsystems.com/images/products/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-immunohistochemistry-152025103033..jpg "Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] -")
Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] -
Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Tau (p Thr181) antibody [HL2498] detects Tau (phospho Thr181) protein in the cytoplasm by immunohistochemical analysis in the presence of non phospho control peptide (left) but not in the presence of the antigenic phospho peptide (right).Sample: Paraffin-embedded mouse brain.
Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2498] diluted at 1:100.
![Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free](https://beta-resources.rndsystems.com/images/products/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-elisa-152025104331..jpg "ELISA: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] -")
ELISA: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] -
ELISA: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below.Capture: Tau (phospho Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)
Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)
![Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free](https://beta-resources.rndsystems.com/images/products/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-elisa-152025105927..jpg "ELISA: Rabbit Tau [p Thr181] mAb (HL2498) -")
ELISA: Rabbit Tau [p Thr181] mAb (HL2498) -
ELISA: Rabbit Tau [p Thr181] mAb (HL2498) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.Capture: Tau (p Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)
Detection: HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)
Applications for Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tau
Long Name
Microtubule-Associated Protein Tau
Alternate Names
MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND
Gene Symbol
MAPT
Additional Tau Products
Product Documents for Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free
Product Specific Notices for Tau [p Thr181] Antibody (HL2498) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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