TAZ/WWTR1 Antibody (CL0371) - BSA Free

Novus Biologicals | Catalog # NBP2-52926

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0371

Format

BSA Free
View all TAZ/WWTR1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9GZV5, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TAZ/WWTR1 Antibody (CL0371) - BSA Free

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining in human placenta and lymph node tissues. Corresponding WWTR1 RNA-seq data are presented for the same tissues.
Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-WWTR1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining in SKMEL cell line with Anti-WWTR1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.
Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human placenta shows strong nuclear and some cytoplasmic positivity in endothelial cells.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules and in glomeruli.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human lymph node shows no nuclear positivity in lymphoid cells as expected.

Applications for TAZ/WWTR1 Antibody (CL0371) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TAZ/WWTR1

TAZ is a YAP-related protein that contains a WW domain and transcriptional activation region. TAZ plays a role in mesenchymal stem cell differentiation. The antibody was designed in such a way that should generate a TAZ specific-reagent able to differentiate between YAP and TAZ proteins in cells and tissues.

Long Name

Transcriptional Coactivator with PDZ-binding Motif

Alternate Names

WWTR1

Gene Symbol

WWTR1

Additional TAZ/WWTR1 Products

Product Documents for TAZ/WWTR1 Antibody (CL0371) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TAZ/WWTR1 Antibody (CL0371) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TAZ/WWTR1 Antibody (CL0371) - BSA Free

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  • Q: I want to know which TAZ primary antibody is best produced for WB (human and mouse)!

    A:

    I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.

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