Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Proximity Ligation Assay

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TAZ/WWTR1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides made to the human TAZ protein. These peptides were selected for the lack of homology to YAP protein. [UniProt# Q9GZV5]

Localization

Nuclear and cytoplasmic. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TAZ/WWTR1 Antibody - BSA Free (NB110-58359) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western, IP and ChIP. Anti-TAZ/WWTR1 Antibody: Cited in 20 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TAZ/WWTR1 Antibody - BSA Free

Western Blot: TAZ/WWTR1 AntibodyBSA Free [NB110-58359]

Western Blot: TAZ/WWTR1 AntibodyBSA Free [NB110-58359]

Western Blot: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ on HEK 293 cell lysate using NB110-58359.
Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody - BSA Free [NB110-58359]

Immunocytochemistry/ Immunofluorescence: TAZ/WWTR1 Antibody - BSA Free [NB110-58359]

Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody [NB110-58359] - Detection of TAZ (Green) in HepG2 cells using NB110-58359. Nuclei (Blue) are counterstained with Hoecsht 33258.
Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody - BSA Free [NB110-58359]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody - BSA Free [NB110-58359]

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NB110-58359] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using TAZ/WWTR1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear and cytoplasmic staining was observed. Staining was performed by Histowiz.
Simple Western: TAZ/WWTR1 AntibodyBSA Free [NB110-58359]

Simple Western: TAZ/WWTR1 AntibodyBSA Free [NB110-58359]

Simple Western: TAZ/WWTR1 Antibody [NB110-58359] - Simple Western lane view shows a specific band for TAZ in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
TAZ/WWTR1 Antibody - BSA Free

Immunoprecipitation: TAZ/WWTR1 Antibody - BSA Free [NB110-58359] -

Immunoprecipitation: TAZ/WWTR1 Antibody - BSA Free [NB110-58359] - TAZ knockout in muscle decreases mitochondrial mass, respiration, & exercise ability. b Proteins of gastrocnemius muscle of WT & mKO mice analysed via immunoblotting for detection of mitochondrial marker proteins. Alpha-tubulin used as loading control. Representative data shown & experiment performed twice w/ similar results. Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/35115527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TAZ/WWTR1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 25587023)

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500. Use reported in scientific literature (PMID 25587023)

Immunoprecipitation

1:100

Proximity Ligation Assay

reported in scientific literature (PMID 25587023)

Simple Western

1:100

Western Blot

1:1000-1:5000
Application Notes

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 58 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 3 using NB110-58359 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TAZ/WWTR1

TAZ (transcriptional co-activator with PDZ-binding motif) was identified as a binding partner of 14-3-3 family of proteins which contains seven homologous proteins with ability to bind phosphorylated serine with certain sequence motifs, thereby involving in diverse cellular functions such as differentiation, cell cycle progression and apoptosis via their interaction with diverse intracellular phosphoproteins involved in signaling networks. TAZ is homologous to YAP and displays transcriptional co-activator function via interaction with PPXY-containing transcriptional factors through its WW domain. Several transcriptional factors such as Runx/PEBP2, AP2, C/EBP, c-Jun, Krox-20, Krox-24, MEF2B, NF-E2, Oct-4 and p73 containing proline-rich PPXY motif have been proposed as TAZ's interacting partners. TAZ is also a major target of the Hippo core kinase cascade which regulates organ size control as well as stem cell properties by governing cell proliferation and apoptosis processes. Hippo mediated phosphorylation of YAP and TAZ leads to their sequestration into the cytoplasm by interaction with 14-3-3 proteins and ubiquitination-dependent proteosomal degradation, thereby governing its distribution, protein levels and functionality. TAZ interacts primarily with transcriptional factors TEAD1-4 (TEADs) and activates expression of target genes such as CTGF, IGFBP3, ITGB2, Birc5/Survivin, Gli2, and Axl.

Long Name

Transcriptional Coactivator with PDZ-binding Motif

Alternate Names

WWTR1

Entrez Gene IDs

25937 (Human)

Gene Symbol

WWTR1

UniProt

Q9GZV5 (Human)

Additional TAZ/WWTR1 Products

Product Documents for TAZ/WWTR1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TAZ/WWTR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TAZ/WWTR1 Antibody - BSA Free

Customer Reviews for TAZ/WWTR1 Antibody - BSA Free (1)

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Customer Images

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  • Name: Andrew Cosgrove
    Application: Western Blot
    Sample Tested: HEK293T cell lysate
    Species: Human
    Verified Customer | Posted 11/06/2013
    TAZ/WWTR1 Antibody - BSA Free NB110-58359

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Protocols

View specific protocols for TAZ/WWTR1 Antibody - BSA Free (NB110-58359):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TAZ/WWTR1 Antibody - BSA Free

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    • Q: I am looking for a WWTR1 antibody and NB110-58359 looks like the best one. I wonder if you guys can provide me the target sequence information of the antibody?
      A: WWTR1 antibody (NB110-58359) is generated against residues 178-197 of WWTR1 (immunogen accession number Q9GZV5). The exact immunogen sequence for this antibody is: CPQRSMAVSQPNLVMNHQHQQ

    • A: I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.

    • Q: Our customer was interested in the TAZ Antibody (Cat.NB110-58359). Could you please help to confirm if it has been tested with rat cells?
      A: The TAZ antibody, NB110-58359, has been tested and validated for use in rat and is therefore guaranteed. Our 100% guarantee covers all of our products for the listed species and applications.

    • Q: We have an inquiry for your product #NB110-58359, TAZ Antibody. Our customer would like to try this product with rat cells lysate. Could you please let me know if you have data image of rat cells lysate can provide?
      A: This antibody has been validated and is therefore guaranteed for use in human, rat and mouse in the following applications: Western blot, Immunoprecipitation and ICC/IF. We do not currently have a WB image that we can share however rat is one of the guaranteed species.
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