TBARS Parameter Assay Kit

  (4 citations)
(2 Reviews)
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (4)
Supplemental Products
  • Format
    96-well strip plate
  • Assay Length
    2-3 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (150 uL), Cell Lysates (150 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (150 uL)
  • Sensitivity
    0.055 uM
  • Assay Range
    0.0 - 17 uM
  • Specificity
    TBARS in various samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC164 , Parameter Immunoassay Control Set 752 for TBARS - Please Inquire
Product Summary
The Parameter TBARS assay is a 2-3 hour chemical analysis designed to measure TBARS in cell culture supernates, cell lysates, serum, plasma, and urine.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 2.57 6.07 9.26 2.39 5.71 8.76
Standard Deviation 0.025 0.078 0.109 0.125 0.242 0.322
CV% 1 1.3 1.2 5.2 4.2 3.7


The recovery of MDA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 94-110
Cell Lysis Buffer 3 (n=1) 99 96-103
EDTA Plasma (n=4) 91 89-106
Heparin Plasma (n=4) 99 94-108
Serum (n=4) 98 87-105
Urine (n=4) 99 91-106
To assess the linearity of the assay, samples were spiked with high concentrations of MDA, acid treated, and serially diluted with deionized water to produce samples with values within the dynamic range of the assay.
 TBARS/Thiobarbituric Acid Reactive Substances [Biotin]
Product Datasheets

Certificate of Analysis Lookup

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, TBARS standard, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 150 µL Standards and Samples
  4.   Add 150 µL of standards and samples to each well.

  5. 75 µL TBA Reagent
  6.   Add 75 µL of TBA Reagent to each well.
  7.   Pre-read the optical density of each well using a microplate reader set to 532 nm.
  8.   Incubate the microplate for 2-3 hours at 45-50 °C.
  9.   Read the optical density of each well using a microplate reader set to 532 nm. Subtract the pre-reading from the final reading to correct for the sample's contributions to the final absorption at 532 nm.

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Sample Type
  1. Protective Effects of Curcumin against Sodium Arsenite-induced Ovarian Oxidative Injury in a Mouse Model
    Authors: XN Wang, CJ Zhang, HL Diao, Y Zhang
    Chin. Med. J., 2017;130(9):1026-1032.
    Species: Mouse
    Sample Type: Tissue Homogenates
  2. Protective effect of active perfusion in porcine models of acute myocardial ischemia
    Mol Med Rep, 2016;14(4):3581-7.
    Species: Porcine
    Sample Type: Serum
  3. Shape-Related Toxicity of Titanium Dioxide Nanofibres
    Authors: M Allegri, MG Bianchi, M Chiu, J Varet, AL Costa, S Ortelli, M Blosi, O Bussolati, CA Poland, E Bergamasch
    PLoS ONE, 2016;11(3):e0151365.
    Species: Mouse
    Sample Type: Cell Lysates
  4. The Effect of Krill Oil Supplementation on Exercise Performance and Markers of Immune Function.
    Authors: Da Boit M, Mastalurova I, Brazaite G, McGovern N, Thompson K, Gray S
    PLoS ONE, 2015;10(9):e0139174.
    Species: Human
    Sample Type: Plasma


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Parameter Immunoassay Control Set 752 for TBARS

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Average Rating: 5 (Based on 2 reviews)

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Images Ratings Applications Species Reviewed By Date Details
 TBARS Parameter Assay Kit KGE013 Array KGE013
  Anonymous 07/13/2017
Image Details
 TBARS Parameter Assay Kit KGE013
: TBARS Parameter Assay Kit [KGE013].


Sample Tested Urine

Other Experimental Details

Other Experimental Details Fast and reliable
  Anonymous 10/09/2016


Sample Tested 2120Ep human embryonal carcinoma cell line

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