TBARS Parameter Assay Kit

R&D Systems | Catalog # KGE013

R&D Systems
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Key Product Details

Assay Length

2-3 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (150 µL), Cell Lysates (150 µL), Serum (150 µL), EDTA Plasma (150 µL), Heparin Plasma (150 µL), Urine (150 µL)

Sensitivity

0.055 µM

Assay Range

0-16.7 µM
View all TBARS/Thiobarbituric Acid Reactive Substances ELISA Kits »

Product Summary for TBARS Parameter Assay Kit

The Parameter TBARS assay is a 2-3 hour chemical analysis designed to measure TBARS in cell culture supernates, cell lysates, serum, plasma, and urine.

Product Specifications

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Species

Multi-Species

Specificity

TBARS in various samples

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (µM) 2.57 6.07 9.26 2.39 5.71 8.76
Standard Deviation 0.025 0.078 0.109 0.125 0.242 0.322
CV% 1.0 1.3 1.2 5.2 4.2 3.7

Recovery for TBARS Parameter Assay Kit

The recovery of MDA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 94-110
Cell Lysis Buffer 3 (n=1) 99 96-103
EDTA Plasma (n=4) 91 89-106
Heparin Plasma (n=4) 99 94-108
Serum (n=4) 98 87-105
Urine (n=4) 99 91-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of MDA, acid treated, and serially diluted with deionized water to produce samples with values within the dynamic range of the assay.

Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Linearity

Scientific Data Images for TBARS Parameter Assay Kit

Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Standard Curve

Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TBARS/Thiobarbituric Acid Reactive Substances

Alternate Names

Thiobarbituric Acid Reactive Substances

Additional TBARS/Thiobarbituric Acid Reactive Substances Products

Product Documents for TBARS Parameter Assay Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for TBARS Parameter Assay Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

Citations for TBARS Parameter Assay Kit

Customer Reviews for TBARS Parameter Assay Kit (3)

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  • TBARS Parameter Assay Kit
    Name: Mary-Kate Hayward
    Sample Tested: Mouse tumor
    Verified Customer | Posted 03/22/2024
    Performed using mouse tumor tissue homogenized in RIPA buffer. Protocol easy to follow and performed well
  • TBARS Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 07/13/2017
    Fast and reliable
    TBARS Parameter Assay Kit KGE013
  • TBARS Parameter Assay Kit
    Name: muthannathb thb
    Sample Tested: 2120Ep human embryonal carcinoma cell line
    Verified Customer | Posted 10/09/2016

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Protocols

View specific protocols for TBARS Parameter Assay Kit (KGE013):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, TBARS standard, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 150 µL Standards and Samples
  3.   Add 150 µL of standards and samples to each well.
    4. 75 µL TBA Reagent
  4.   Add 75 µL of TBA Reagent to each well.
  5.   Pre-read the optical density of each well using a microplate reader set to 532 nm.
  6.   Incubate the microplate for 2-3 hours at 45-50 °C.
  7.   Read the optical density of each well using a microplate reader set to 532 nm. Subtract the pre-reading from the final reading to correct for the sample's contributions to the final absorption at 532 nm.

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