TBARS Parameter Assay Kit

Catalog # Availability Size / Price Qty
KGE013
Control Products Available
Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Standard Curve
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Product Details
Procedure
Citations (37)
FAQs
Supplemental Products
Reviews (2)

TBARS Parameter Assay Kit Summary

Format
96-well strip plate
Assay Length
2-3 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (150 uL), Cell Lysates (150 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (150 uL)
Sensitivity
0.055 uM
Assay Range
0.0 - 17 uM
Specificity
TBARS in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

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Product Summary

The Parameter TBARS assay is a 2-3 hour chemical analysis designed to measure TBARS in cell culture supernates, cell lysates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (µM) 2.57 6.07 9.26 2.39 5.71 8.76
Standard Deviation 0.025 0.078 0.109 0.125 0.242 0.322
CV% 1 1.3 1.2 5.2 4.2 3.7

Recovery

The recovery of MDA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 94-110
Cell Lysis Buffer 3 (n=1) 99 96-103
EDTA Plasma (n=4) 91 89-106
Heparin Plasma (n=4) 99 94-108
Serum (n=4) 98 87-105
Urine (n=4) 99 91-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of MDA, acid treated, and serially diluted with deionized water to produce samples with values within the dynamic range of the assay.
Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Linearity

Scientific Data

Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, TBARS standard, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 150 µL Standards and Samples
  4.   Add 150 µL of standards and samples to each well.

  5. 75 µL TBA Reagent
  6.   Add 75 µL of TBA Reagent to each well.
  7.   Pre-read the optical density of each well using a microplate reader set to 532 nm.
  8.   Incubate the microplate for 2-3 hours at 45-50 °C.
  9.   Read the optical density of each well using a microplate reader set to 532 nm. Subtract the pre-reading from the final reading to correct for the sample's contributions to the final absorption at 532 nm.

Citations for TBARS Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Paraoxonase-2 contributes to promoting lipid metabolism and mitochondrial function via autophagy activation
    Authors: GC Shin, HM Lee, N Kim, SK Yoo, HS Park, LS Choi, KP Kim, AR Lee, SU Seo, KH Kim
    Scientific Reports, 2022;12(1):21483.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Minocycline relieves myocardial ischemia-reperfusion injury in rats by inhibiting inflammation, oxidative stress and apoptosis
    Authors: LQ Chen, WS Wang, SQ Li, JH Liu
    European review for medical and pharmacological sciences, 2022;26(8):3001-3009.
    Species: Rat
    Sample Types: Serum
  3. Fxyd5 activates the NF?kappaB pathway and is involved in chondrocytes inflammation and extracellular matrix degradation
    Authors: L Song, X Li, Q Sun, Y Zhao
    Molecular Medicine Reports, 2022;25(4):.
    Species: Mouse
    Sample Types: Cell Lysates
  4. Co-Administration of Iron and Bioavailable Curcumin Reduces Levels of Systemic Markers of Inflammation and Oxidative Stress in a Placebo-Controlled Randomised Study
    Authors: H Tiekou Lor, G Begum, L Temouri, D Renshaw, MG Zariwala
    Nutrients, 2022;14(3):.
    Species: Human
    Sample Types: Serum
  5. Acute effects of exposure to fine particulate matter and ozone on lung function, inflammation and oxidative stress in healthy adults
    Authors: R Xu, Z Li, X Zhu, C Guo, Q Su, J Peng, Z Wang, Y Qian, X Li, Q Xu, Y Wei
    Ecotoxicology and environmental safety, 2022;243(0):114013.
    Species: Human
    Sample Types: Plasma
  6. Dexpramipexole Attenuates White Matter Injury to Facilitate Locomotion and Motor Coordination Recovery via Reducing Ferroptosis after Intracerebral Hemorrhage
    Authors: B Wang, X Zhang, J Zhong, S Wang, C Zhang, M Li, Q Hu, S Wang, L Chen, W Chen, H Ge, H Feng
    Oxidative Medicine and Cellular Longevity, 2022;2022(0):6160701.
    Species: Mouse
    Sample Types: Tissue Lysate
  7. Comparison of the Effects of Three Dual-Nucleos(t)ide Reverse Transcriptase Inhibitor Backbones on Placenta Mitochondria Toxicity and Oxidative Stress Using a Mouse Pregnancy Model
    Authors: K Balogun, L Serghides
    Pharmaceutics, 2022;14(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Mangiferin exert protective effects on joints of adjuvant-induced arthritis rats by regulating the MAPKs/NF-kappaB pathway of fibroblast-like synoviocytes
    Authors: R Wang, J Liu, Z Wang, X Wu, H Guo, X Jiao, H Zhang, C Qi, X Li
    International immunopharmacology, 2021;0(0):108352.
    Species: Rat
    Sample Types: Plasma
  9. Modulation of COX-2 and NADPH oxidase-4 by alpha-lipoic acid ameliorates busulfan-induced pulmonary injury in rats
    Authors: MG Elhadidy, A Elmasry, HRH Elsayed, M El-Nablawa, S Hamed, MM Elalfy, MR Rabei
    Heliyon, 2021;7(10):e08171.
    Species: Rat
    Sample Types: Tissue Homogenates
  10. Ellagic Acid Modulates the Amyloid Precursor Protein Gene via Superoxide Dismutase Regulation in the Entorhinal Cortex in an Experimental Alzheimer's Model
    Authors: WS Ramadan, S Alkarim
    Cells, 2021;10(12):.
    Species: Rat
    Sample Types: Serum
  11. Effects of the brominated flame retardant, TBCO, on development of zebrafish (Danio rerio) embryos
    Authors: D Van Essen, C Devoy, J Miller, PD Jones, S Wiseman
    Chemosphere, 2021;266(0):129195.
    Species: Zebrafish
    Sample Types: Embryo Homogenate
  12. Acute Administration of Bioavailable Curcumin Alongside Ferrous Sulphate Supplements Does Not Impair Iron Absorption in Healthy Adults in a Randomised Trial
    Authors: HT Lorinczova, G Begum, D Renshaw, MG Zariwala
    Nutrients, 2021;13(7):.
    Species: Human
    Sample Types: Serum
  13. Excessive Iron Induces Oxidative Stress Promoting Cellular Perturbations and Insulin Secretory Dysfunction in MIN6 Beta Cells
    Authors: V Blesia, VB Patel, H Al-Obaidi, D Renshaw, MG Zariwala
    Cells, 2021;10(5):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. The impact of maternal obesity and breast milk inflammation on developmental programming of infant growth
    Authors: S Enstad, S Cheema, R Thomas, RN Fichorova, CR Martin, P O'Tierney-, CL Wagner, S Sen
    Eur J Clin Nutr, 2020;0(0):.
    Species: Human
    Sample Types: Breast Milk
  15. Active food ingredients production from cold pressed processing residues of Camellia oleifera and Camellia sinensis seeds for regulation of blood pressure and vascular function
    Authors: SS Chiang, LS Chen, CY Chu
    Chemosphere, 2020;267(0):129267.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. Unripe Rubus coreanus Miquel Extract Containing Ellagic Acid Regulates AMPK, SREBP-2, HMGCR, and INSIG-1 Signaling and Cholesterol Metabolism In Vitro and In Vivo
    Authors: KH Lee, ES Jeong, G Jang, JR Na, S Park, WS Kang, E Kim, H Choi, JS Kim, S Kim
    Nutrients, 2020;12(3):.
    Species: Rat
    Sample Types: Serum
  17. Effects of the brominated flame retardant, TBCO, on development of zebrafish (Danio rerio) embryos
    Authors: D Van Essen, C Devoy, J Miller, PD Jones, S Wiseman
    Chemosphere, 2020;266(0):129195.
    Species: Zebrafish
    Sample Types: Embryo
  18. The impact of maternal obesity and breast milk inflammation on developmental programming of infant growth
    Authors: S Enstad, S Cheema, R Thomas, RN Fichorova, CR Martin, P O'Tierney-, CL Wagner, S Sen
    Eur J Clin Nutr, 2020;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  19. Nitric oxide and oxidative stress pathways do not contribute to sex differences in renal injury and function in Dahl SS/Jr rats
    Authors: HR Turbeville, AC Johnson, MR Garrett, EL Dent, JM Sasser
    Physiol Rep, 2020;8(13):e14440.
    Species: Rat
    Sample Types: Urine
  20. Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
    Authors: XJ Chen, SM Ren, JZ Dong, CG Qiu, YW Chen, HL Tao
    Drug Des Devel Ther, 2019;13(0):647-655.
    Species: Rat
    Sample Types: Tissue Homogenates
  21. Vinyl chloride-induced interaction of nonalcoholic and toxicant-associated steatohepatitis: Protection by the ALDH2 activator Alda-1
    Authors: L Chen, AL Lang, GD Poff, WX Ding, JI Beier
    Redox Biol, 2019;24(0):101205.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. STC-1 ameliorates renal injury in diabetic nephropathy by inhibiting the expression of BNIP3 through the AMPK/SIRT3 pathway
    Authors: Z Liu, H Liu, L Xiao, G Liu, L Sun, L He
    Lab. Invest., 2019;0(0):.
    Species: Human
    Sample Types: Serum
  23. Dendropanax morbifera Ameliorates Thioacetamide-Induced Hepatic Fibrosis via TGF-?1/Smads Pathways
    Authors: HY Yang, KS Kim, YH Lee, JH Park, JH Kim, SY Lee, YM Kim, IS Kim, S Kacew, BM Lee, JH Kwak, K Yoon, HS Kim
    Int. J. Biol. Sci., 2019;15(4):800-811.
    Species: Rat
    Sample Types: Tissue Homogenates
  24. The role of fish oil in attenuating cardiac oxidative stress, inflammation and fibrosis in rat model of thyrotoxicosis
    Authors: F Mayyas, A Alsaheb, KH Alzoubi
    Heliyon, 2019;5(12):e02976.
    Species: Rat
    Sample Types: Serum
  25. Palmitic acid induces neurotoxicity and gliatoxicity in SH-SY5Y human neuroblastoma and T98G human glioblastoma cells
    Authors: YW Ng, YH Say
    PeerJ, 2018;6(0):e4696.
    Species: Human
    Sample Types: Cell Lysates
  26. Cardiac effects of fish oil in a rat model of streptozotocin-induced diabetes
    Authors: F Mayyas, R Jaradat, KH Alzoubi
    Nutr Metab Cardiovasc Dis, 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  27. Porphyromonas gingivalis accelerates atherosclerosis through oxidation of high-density lipoprotein
    Authors: HJ Kim, GS Cha, HJ Kim, EY Kwon, JY Lee, J Choi, JY Joo
    J Periodontal Implant Sci, 2018;48(1):60-68.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Puerarin protects against heart failure induced by pressure overload through mitigation of ferroptosis
    Authors: B Liu, C Zhao, H Li, X Chen, Y Ding, S Xu
    Biochem. Biophys. Res. Commun., 2018;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
  29. Pretreatment of ferulic acid attenuates inflammation and oxidative stress in a rat model of lipopolysaccharide-induced acute respiratory distress syndrome
    Authors: S Zhang, P Wang, P Zhao, D Wang, Y Zhang, J Wang, L Chen, W Guo, H Gao, Y Jiao
    Int J Immunopathol Pharmacol, 2018;32(0):3946320177505.
    Species: Rat
    Sample Types: Tissue Homogenates
  30. The Effect of Whole-Body Cryotherapy at Different Temperatures on Proinflammatory Cytokines, Oxidative Stress Parameters, and Disease Activity in Patients with Ankylosing Spondylitis
    Authors: A Straburzy?, MP Kasprzak, MW Romanowski, A Kwa?niewsk, W Romanowski, M Iskra, R Rutkowski
    Oxid Med Cell Longev, 2018;2018(0):2157496.
    Species: Human
    Sample Types: Serum
  31. Bone morphogenetic protein 6 (BMP-6) modulates lung function, pulmonary iron levels and cigarette smoke-induced inflammation
    Authors: FM Verhamme, EG De Smet, W Van Hooste, J Delanghe, SE Verleden, GF Joos, GG Brusselle, KR Bracke
    Mucosal Immunol, 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. Efavirenz exposure, alone and in combination with known drugs of abuse, engenders addictive-like bio-behavioural changes in rats
    Authors: M Möller, J Fourie, BH Harvey
    Sci Rep, 2018;8(1):12837.
    Species: Rat
    Sample Types: Tissue Homogenates
  33. Randomized trial of red cell washing for the prevention of transfusion-associated organ injury in cardiac surgery
    Authors: MJ Wozniak, N Sullo, S Qureshi, W Dott, R Cardigan, M Wiltshire, T Morris, M Nath, N Bittar, SK Bhudia, T Kumar, AH Goodall, GJ Murphy
    Br J Anaesth, 2017;118(5):689-698.
    Species: Human
    Sample Types: Serum
  34. Protective Effects of Curcumin against Sodium Arsenite-induced Ovarian Oxidative Injury in a Mouse Model
    Authors: XN Wang, CJ Zhang, HL Diao, Y Zhang
    Chin. Med. J., 2017;130(9):1026-1032.
    Species: Mouse
    Sample Types: Tissue Homogenates
  35. Protective effect of active perfusion in porcine models of acute myocardial ischemia
    Mol Med Rep, 2016;14(4):3581-7.
    Species: Porcine
    Sample Types: Serum
  36. Shape-Related Toxicity of Titanium Dioxide Nanofibres
    Authors: M Allegri, MG Bianchi, M Chiu, J Varet, AL Costa, S Ortelli, M Blosi, O Bussolati, CA Poland, E Bergamasch
    PLoS ONE, 2016;11(3):e0151365.
    Species: Mouse
    Sample Types: Cell Lysates
  37. The Effect of Krill Oil Supplementation on Exercise Performance and Markers of Immune Function.
    Authors: Da Boit M, Mastalurova I, Brazaite G, McGovern N, Thompson K, Gray S
    PLoS ONE, 2015;10(9):e0139174.
    Species: Human
    Sample Types: Plasma

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TBARS Parameter Assay Kit
By Anonymous on 07/13/2017
Sample Tested: Urine

Fast and reliable


TBARS Parameter Assay Kit
By muthannathb thb on 10/09/2016
Sample Tested: 2120Ep human embryonal carcinoma cell line