TBK1 Antibody (108A429) - BSA Free

Novus Biologicals | Catalog # NB100-56705

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine

Cited:

Human, Mouse, Rat

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 108A429

Format

BSA Free
View all TBK1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TBK1 Antibody (108A429) - BSA Free

Knockout Validated: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Western Blot: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Western blot using lysates from U20S parental cell line and TBK1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse/Rat TBK1 Monoclonal Antibody (Catalog # NB100-56705) at 1 ug/ul O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band at 83 kDa was detected for TBK1 in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Simple Western: TBK1 Antibody (108A429)BSA Free [NB100-56705]

Simple Western: TBK1 Antibody (108A429)BSA Free [NB100-56705]

Simple Western: TBK1 Antibody (108A429) [NB100-56705] - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.
Western Blot: TBK1 Antibody (108A429)BSA Free [NB100-56705]

Western Blot: TBK1 Antibody (108A429)BSA Free [NB100-56705]

Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis using Azide and BSA Free version of NB100-56705. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) Image from the Azide and BSA Free version of this antibody.
Western Blot: TBK1 Antibody (108A429)BSA Free [NB100-56705]

Western Blot: TBK1 Antibody (108A429)BSA Free [NB100-56705]

Western Blot: TBK1 Antibody (108A429) [NB100-56705] - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Immunocytochemistry/ Immunofluorescence: TBK1 Antibody (108A429) - BSA Free [NB100-56705]

Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TBK1 at 20 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
TBK1 Antibody (108A429) - BSA Free

Western Blot: TBK1 Antibody (108A429) - BSA Free [NB100-56705] -

STING pathway analysis. (A) STING mRNA relative expression in uninfected (CTR), HHV-6A, HHV-6B or HHV-7 infected NK92 cells 3d.p.i. ∗p values Student t test; (B) Western Blot analysis for house-keeping actin (upper blot) and STING (lower blot) expression in uninfected (CTR), HHV-6A, HHV-6B or HHV-7 infected NK92 cells 3d.p.i. For stimulation of the cytoplasmic DNA sensing pathways, we used 2′,3′-cGAMP. The molecular weights were determined by protein ladder (14.4-97.4kDa) (BioRad). Actin was evidenced at 44kDa, STING 35kDa. The images were acquired by Geliance 600 (Perkin Elmer, MA, United States). The complete Western Blots are reported in Supplementary Figure S3. ∗p value Student t test. The histogram represents the STING band intensity in relation with the corresponding actin band. (C) Western Blot analysis for house-keeping actin (upper blot) and cGas, IFI16, TBK1, IRF3, pIRF3Ser396, STAT6, pSTAT6Tyr641 (lower blots) expression in uninfected (CTR), HHV-6A, HHV-6B or HHV-7 infected NK92 cells 3 d.p.i. The molecular weights were determined by protein ladder (25-250kDa; 14.4-97.4 kDa). Actin was evidenced at 44kDa, cGas at 68kDa, IFI16 at 100kDa, TBK1 at 80kDa, IRF3 at 55kDa, STAT6 at 120kDa. The histogram represents the STING band intensity in relation with the corresponding actin band. ∗p value Student t test. The images were acquired by Geliance 600 (Perkin Elmer, MA, United States). The complete Western Blots are reported in Supplementary Figure S3. (D) HHV-6A infected NK92 cells were characterized by immunofluorescence for STING [anti-STING PE Ab (Clone T3-680)], STAT6 [anti-STAT6 FITC (Clone D-1)] and gp116 (Clone 6A5) expression. (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification 100×. Uninfected NK92 cells were used as control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32140147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TBK1 Antibody (108A429) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
In Western Blot this antibody detects a band at 80 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Daudi and Raw 2647 lysate 0.5 mg/mL, separated by Size, antibody dilution of 10 ug/mL. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 5 using NB100-56705 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TBK1

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.

Long Name

TANK Binding Kinase 1

Alternate Names

FTDALS4, NAK, NFKB-Activating Kinase, NAK Antibody, NAK serine antibody, TANK antibody, TANK binding kinase antibody

Entrez Gene IDs

29110 (Human); 56480 (Mouse); 299827 (Rat)

Gene Symbol

TBK1

UniProt

Q9UHD2

Additional TBK1 Products

Product Documents for TBK1 Antibody (108A429) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TBK1 Antibody (108A429) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TBK1 Antibody (108A429) - BSA Free

Customer Reviews for TBK1 Antibody (108A429) - BSA Free (2)

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  • TBK1 Antibody (108A429)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human Corneal Fibroblast Cells
    Species: Human
    Verified Customer | Posted 08/10/2021
    TBK1 Antibody. Human Corneal Fibroblast Cells. Western blot.
    TBK1 Antibody (108A429) - BSA Free NB100-56705
  • TBK1 Antibody (108A429)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293 whole cell lysate
    Species: Human
    Verified Customer | Posted 12/21/2017
    293 T cells were transfected with TBK1 vector for 24h, whole cell lysate was prepared using RIPA and run on western blot. 1:2000 dilution of antibody used.
    TBK1 Antibody (108A429) - BSA Free NB100-56705

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Protocols

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FAQs for TBK1 Antibody (108A429) - BSA Free

Showing  1 - 5 of 7 FAQs Showing All

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

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