TC21/R-Ras2 Antibody (2D3-4B8) - Azide and BSA Free

Novus Biologicals | Catalog # H00022800-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D3-4B8

Format

Azide and BSA Free
View all TC21/R-Ras2 Primary Antibodies »

Product Specifications

Immunogen

RRAS2 (AAH13106, 1 a.a. ~ 204 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF

Localization

Cell membrane; Lipid-anchor; Cytoplasmic side.

Specificity

RRAS2 - related RAS viral (r-ras) oncogene homolog 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TC21/R-Ras2 Antibody (2D3-4B8) - Azide and BSA Free

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Analysis of RRAS2 expression in transfected 293T cell line by RRAS2 monoclonal antibody (M01), clone 2D3-4B8.Lane 1: RRAS2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Immunocytochemistry/ Immunofluorescence: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Immunocytochemistry/Immunofluorescence: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Analysis of monoclonal antibody to RRAS2 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Immunohistochemistry-Paraffin: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Immunohistochemistry-Paraffin: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Analysis of monoclonal antibody to RRAS2 on formalin-fixed paraffin-embedded human dysgerminoma tissue. Antibody concentration 5 ug/ml.
Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8. Analysis of RRAS2 expression in HeLa.
Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8. Analysis of RRAS2 expression in PC-12.
Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8. Analysis of RRAS2 expression in Raw 264.7.
Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8 Analysis of RRAS2 expression in A-431.
Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - RRAS2 monoclonal antibody (M01), clone 2D3-4B8. Analysis of RRAS2 expression in NIH/3T3.
ELISA: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

ELISA: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01]

ELISA: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Detection limit for recombinant GST tagged RRAS2 is 1 ng/ml as a capture antibody.
TC21/R-Ras2 Antibody (2D3-4B8)

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] -

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Validation of R-Ras2 as the defatty-acylation target of SIRT6.(A) mRNA & protein levels of R-Ras2 in Sirt6 WT & KO MEFs. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/25158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TC21/R-Ras2 Antibody (2D3-4B8)

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] -

Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Identification of defatty-acylation targets of SIRT6 that contribute to its function in cell proliferation.(A) Cell proliferation of Sirt6 KO MEFs stably expressing pCDH empty vector, SIRT6 WT, G60A, or H133Y. Cell proliferation was assayed & quantified using crystal violet staining. Values with error bars indicate mean ± s.d. of three biological replicates. * indicates p<0.05 & ‘ns’ indicates no statistical significance when comparing to Sirt6 KO MEFs with pCDH. (B) Schematic overview of the SILAC experiment to identify SIRT6 lysine defatty-acylation targets. (C) Data analysis & filter of SILAC results. (D) Effects of R-Ras2 knockdown on the proliferation of Sirt6 WT & KO MEFs. Values with error bars indicate mean ± s.d. of three biological replicates. *** indicates p<0.005 for comparing to Sirt6 KO MEFs with ctrl shRNA.DOI:http://dx.doi.org/10.7554/eLife.25158.003Schematic overview of the SILAC experiment to compare the protein abundance in Sirt6 WT & KO MEFs.DOI:http://dx.doi.org/10.7554/eLife.25158.004 Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/25158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TC21/R-Ras2 Antibody (2D3-4B8) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TC21/R-Ras2

RRAS2 (Related RAS viral oncogene homolog 2) is a member of the Ras superfamily. It is a GTP-binding protein with GTPase activity. It might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins. RRAS2 has high oncogenic potential and is mutated in some human tumors and overexpressed in breast cancer cell lines.

Long Name

Teratocarcinoma Oncogene TC21

Alternate Names

RRAS2

Entrez Gene IDs

22800 (Human)

Gene Symbol

RRAS2

OMIM

600098 (Human)

UniProt

AAH13106 (Human)

Additional TC21/R-Ras2 Products

Product Documents for TC21/R-Ras2 Antibody (2D3-4B8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TC21/R-Ras2 Antibody (2D3-4B8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TC21/R-Ras2 Antibody (2D3-4B8) - Azide and BSA Free

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Protocols

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FAQs

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