TCP1-eta Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88144

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TCP1-eta Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLEKIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24213632)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCP1-eta Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TCP1-eta Antibody [NBP1-88144]

Immunocytochemistry/ Immunofluorescence: TCP1-eta Antibody [NBP1-88144]

Immunocytochemistry/Immunofluorescence: TCP1-eta Antibody [NBP1-88144] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
TCP1-eta Antibody - BSA Free Western Blot: TCP1-eta Antibody - BSA Free [NBP1-88144]

Western Blot: TCP1-eta Antibody - BSA Free [NBP1-88144]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
TCP1-eta Antibody - BSA Free Immunohistochemistry-Paraffin: TCP1-eta Antibody - BSA Free [NBP1-88144]

Immunohistochemistry-Paraffin: TCP1-eta Antibody - BSA Free [NBP1-88144]

Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
TCP1-eta Antibody - BSA Free Western Blot: TCP1-eta Antibody - BSA Free [NBP1-88144]

Western Blot: TCP1-eta Antibody - BSA Free [NBP1-88144]

Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CCT7 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for TCP1-eta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCP1-eta

TCP1-eta encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants encoding different isoforms have been found for this gene, but only two of them have been characterized to date.

Alternate Names

CCTETA, CCT-eta, Ccth, chaperonin containing TCP1, subunit 7 (eta), eta subunit, HIV-1 Nef interacting protein, HIV-1 Nef-interacting protein, MGC110985, Nip7-1, T-complex protein 1 subunit eta

Gene Symbol

CCT7

Additional TCP1-eta Products

Product Documents for TCP1-eta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TCP1-eta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TCP1-eta Antibody - BSA Free

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Protocols

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