TEAD4 Antibody (5H3) - Azide and BSA Free

Novus Biologicals | Catalog # H00007004-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5H3

Format

Azide and BSA Free
View all TEAD4 Primary Antibodies »

Product Specifications

Immunogen

TEAD4 (NP_003204, 151 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ARGPGRPAVSGFWQGALPGQAGTSHDVKPFSQQTYAVQPPLPLPGFESPAGPAPSPSAPPAPPWQGRSVASSKLWMLEFSAFLEQQQDPDTYNKHLFVHIGQSSPSYSDP

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23195226). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

TEAD4 - TEA domain family member 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TEAD4 Antibody (5H3) - Azide and BSA Free

Western Blot: TEAD4 Antibody (5H3) [H00007004-M01]

Western Blot: TEAD4 Antibody (5H3) [H00007004-M01]

Western Blot: TEAD4 Antibody (5H3) [H00007004-M01] - TEAD4 monoclonal antibody (M01), clone 5H3 Analysis of TEAD4 expression in HeLa.
Western Blot: TEAD4 Antibody (5H3) [H00007004-M01]

Western Blot: TEAD4 Antibody (5H3) [H00007004-M01]

Western Blot: TEAD4 Antibody (5H3) [H00007004-M01] - Analysis of TEAD4 over-expressed 293 cell line, cotransfected with TEAD4 Validated Chimera RNAi ( Cat # H00007004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TEAD4 monoclonal antibody (M01), clone 5H3 (Cat # H00007004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: TEAD4 Antibody (5H3) [H00007004-M01]

Western Blot: TEAD4 Antibody (5H3) [H00007004-M01]

Western Blot: TEAD4 Antibody (5H3) [H00007004-M01] - Analysis of TEAD4 expression in transfected 293T cell line by TEAD4 monoclonal antibody (M01), clone 5H3.Lane 1: TEAD4 transfected lysate(34.2 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: TEAD4 Antibody (5H3) [H00007004-M01]

Immunoprecipitation: TEAD4 Antibody (5H3) [H00007004-M01]

Immunoprecipitation: TEAD4 Antibody (5H3) [H00007004-M01] - Analysis of TEAD4 transfected lysate using anti-TEAD4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TEAD4 MaxPab rabbit polyclonal antibody.
ELISA: TEAD4 Antibody (5H3) [H00007004-M01]

ELISA: TEAD4 Antibody (5H3) [H00007004-M01]

ELISA: TEAD4 Antibody (5H3) [H00007004-M01] - Detection limit for recombinant GST tagged TEAD4 is approximately 0.03ng/ml as a capture antibody.

Applications for TEAD4 Antibody (5H3) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA. ICC/IF usage reported in scientific literature (PMID: 23195226).

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TEAD4

This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene.

Alternate Names

EFTR-2, hRTEF-1B, related transcription enhancer factor 1B, RTEF1RTEF-1, TCF13L1MGC9014, TEA domain family member 4TEF3, TEAD-4, TEF-3, TEFR-1, Transcription factor 13-like 1, Transcription factor RTEF-1, transcriptional enhancer factor 1-related, transcriptional enhancer factor 3, transcriptional enhancer factor TEF-3

Entrez Gene IDs

7004 (Human)

Gene Symbol

TEAD4

OMIM

601714 (Human)

UniProt

NP_003204 (Human)

Additional TEAD4 Products

Product Documents for TEAD4 Antibody (5H3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TEAD4 Antibody (5H3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TEAD4 Antibody (5H3) - Azide and BSA Free

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Protocols

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FAQs

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