TET1 Antibody (1462) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-15135

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1462

Format

Azide and BSA Free
View all TET1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34874218).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

235 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TET1 Antibody (1462) - Azide and BSA Free

Western Blot: TET1 Antibody (1462) [NBP2-15135]

Western Blot: TET1 Antibody (1462) [NBP2-15135]

Western Blot: TET1 Antibody (1462) [NBP2-15135] - NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [1462] diluted at 1:500.
Western Blot: TET1 Antibody (1462) [NBP2-15135]

Western Blot: TET1 Antibody (1462) [NBP2-15135]

TET1-Antibody-1462-Western-Blot-NBP2-15135-img0026.jpg
Immunocytochemistry/ Immunofluorescence: TET1 Antibody (1462) [NBP2-15135]

Immunocytochemistry/ Immunofluorescence: TET1 Antibody (1462) [NBP2-15135]

Immunocytochemistry/Immunofluorescence: TET1 Antibody (GT1462) [NBP2-15135] - detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135]

Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135]

Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135] - Mouse testis. TET1 stained by TET1 antibody [1462] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: TET1 Antibody (1462) [NBP2-15135]

Western Blot: TET1 Antibody (1462) [NBP2-15135]

Western Blot: TET1 Antibody (GT1462) [NBP2-15135] - A. 50 ug whole cell lysate/extract from 293T cells transfected with scramble siRNA. B. 50 ug whole cell lysate/extract from TET1-knockdowned 293T cells.
Western Blot: TET1 Antibody (1462) [NBP2-15135]

Western Blot: TET1 Antibody (1462) [NBP2-15135]

Western Blot: TET1 Antibody (1462) [NBP2-15135] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells 5 % SDS-PAGE TET1 antibody dilution: 1:500
Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135]

Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135]

Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135] - HeLa xenograft. TET1 antibody [1462] dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
TET1 Antibody (1462)

Western Blot: TET1 Antibody (1462) [NBP2-15135] -

Western Blot: TET1 Antibody (1462) [NBP2-15135] - Changes in the expression of DNMT1 & TET1. Representative western blots & densitometry analysis of DNMT1 & TET1 levels in untreated or DAC-treated (a) HCT116 monocultures & cocultures with normal BJ or sBJ & (b) HCT116 monocultures grown in conditioned medium from normal BJ or sBJ cells. DNMT1 & TET1 blots in (a) & (b) are taken from different gels. Data are mean ± SEM, n = 3, ∗∗∗p < 0.001, ∗p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30158986), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TET1 Antibody (1462) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TET1

TET1 (Ten-eleven translocation 1), also called methylcytosine dioxygenase TET1 or CXXC6, is one of three members of the human TET protein family of alpha-ketoglutarate oxygenases which includes TET2 and TET3 (1-3). TET1 is an enzyme that plays a role in epigenetic regulation of DNA methylation by conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), and further to 5-formylcystosine (5fC) and 5-carboxylcystosine (5caC) (1-5). The human TET1 protein is 2136 amino acids in length with a theoretical molecular weight of 235 kDa (6). Primary features of the TET1 protein include an N-terminal CXXC domain responsible for binding CpG islands in DNA, and a C-terminal catalytic domain containing a Cys-rich region followed by a double-stranded beta-helix (DSBH) domain that contributes to the mC dioxygenase activity and metal binding (1,2,4,5).

Recent studies have shown a role for TET1 in mediating epigenetic changes, such as DNA methylation status, in response to environmental factors such as food and nutrition, exercise, radiation, and allergens (3). The balance between DNA methylation and demethylation is crucial in health and homeostasis (3,5). In addition to response to environmental exposures, TET1 also plays a role in different diseases and cancer subtypes where it can function as either a tumor suppressor or tumor promoter (2,5). TET1 was originally identified as a fusion partner for the mixed lineage leukemia (MLL) gene in acute myeloid leukemia (AML) (1-3). While TET1 expression is low in AML, it is highly expressed in T-cell acute lymphoblastic leukemia (T-ALL) (2). Similarly, TET1 expression is suppressed in hormone receptor positive breast cancer (HRBC) but elevated in triple negative breast cancer (2). TET1 is a potential therapeutic target in certain cancers, but due to its complex role in different signaling pathways its potential needs to be more widely studied (2). While TET1 is primarily responsible for initiating DNA demethylation, it also functions alongside 5hmc in maintaining pluripotency in embryonic stem cells (ESCs) and can serve as a marker for differentiation (1,2,4,5).

References

1. Tan L, Shi YG. Tet family proteins and 5-hydroxymethylcytosine in development and disease. Development. 2012;139(11):1895-1902. https://doi.org/10.1242/dev.070771

2. Liu W, Wu G, Xiong F, Chen Y. Advances in the DNA methylation hydroxylase TET1. Biomark Res. 2021;9(1):76. https://doi.org/10.1186/s40364-021-00331-7

3. Zhu T, Brown AP, Ji H. The Emerging Role of Ten-Eleven Translocation 1 in Epigenetic Responses to Environmental Exposures. Epigenet Insights. 2020;13:2516865720910155. https://doi.org/10.1177/2516865720910155

4. Melamed P, Yosefzon Y, David C, Tsukerman A, Pnueli L. Tet Enzymes, Variants, and Differential Effects on Function. Front Cell Dev Biol. 2018;6:22. https://doi.org/10.3389/fcell.2018.00022

5. Ma C, Seong H, Liu Y, Yu X, Xu S, Li Y. Ten-eleven translocation proteins (TETs): tumor suppressors or tumor enhancers?. Front Biosci (Landmark Ed). 2021;26(10):895-915. https://doi.org/10.52586/4996

6. Uniprot (Q8NFU7)

Long Name

Methylcytosine dioxygenase TET1

Alternate Names

CXXC6, EC 1.14.11.n2, KIAA1676, LCX

Gene Symbol

TET1

Additional TET1 Products

Product Documents for TET1 Antibody (1462) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TET1 Antibody (1462) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TET1 Antibody (1462) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TET1 Antibody (1462) - Azide and BSA Free

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  • Q: Hello, do you know the localization of Tet1 Ab (1462), Cat# NBP2-15135- nucleus or cytoplasma?

    A: Tet1 is localized in the nucleus.

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