Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all TET1 Primary Antibodies »

Product Specifications

Immunogen

TET1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human TET1. The immunogen is located within amino acids 2030 - 2080 of TET1.

Specificity

TET1 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with TET2 and TET3.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for TET1 Antibody - BSA Free

TET1 Antibody

Immunohistochemistry: TET1 Antibody [NBP3-27892] -

Immunohistochemistry: TET1 Antibody [NBP3-27892] - Figure 7 Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
TET1 Antibody

Immunohistochemistry: TET1 Antibody [NBP3-27892] -

Immunohistochemistry: TET1 Antibody [NBP3-27892] - Figure 8 Immunohistochemistry Validation of TET1 in Rat Liver Tissue Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-TET1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
TET1 Antibody

Immunohistochemistry: TET1 Antibody [NBP3-27892] -

Immunohistochemistry: TET1 Antibody [NBP3-27892] - Figure 6 Immunohistochemistry Validation of TET1 in Human Testis Tissue
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TET1 antibody at 1 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
TET1 Antibody

Western Blot: TET1 Antibody [NBP3-27892] -

Western Blot: TET1 Antibody [NBP3-27892] - Figure 3 WB Validation of TET1 in Rat Tissues
Loading: 15 u of lysate Antibodies: TET1, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
TET1 Antibody

Western Blot: TET1 Antibody [NBP3-27892] -

Western Blot: TET1 Antibody [NBP3-27892] - Figure 2 WB Validation of TET1 in Mouse Tissues
Loading: 15 u of lysate Antibodies: TET1, 1 μ g/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
TET1 Antibody

Western Blot: TET1 Antibody [NBP3-27892] -

Western Blot: TET1 Antibody [NBP3-27892] - Figure 1 WB Validation of TET1 in Human Cell Lines
Loading: 15 u of lysate Antibodies: TET1, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
TET1 Antibody

Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP3-27892] -

Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP3-27892] - Figure 5Immunofluorescence Validation of TET1 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TET1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI antibody (blue).
TET1 Antibody

Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP3-27892] -

Immunocytochemistry/ Immunofluorescence: TET1 Antibody [NBP3-27892] - Figure 4 Immunofluorescence Validation of TET1 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling TET1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).

Applications for TET1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry-Paraffin

1 ug/mL

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TET1

TET1 (Ten-eleven translocation 1), also called methylcytosine dioxygenase TET1 or CXXC6, is one of three members of the human TET protein family of alpha-ketoglutarate oxygenases which includes TET2 and TET3 (1-3). TET1 is an enzyme that plays a role in epigenetic regulation of DNA methylation by conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), and further to 5-formylcystosine (5fC) and 5-carboxylcystosine (5caC) (1-5). The human TET1 protein is 2136 amino acids in length with a theoretical molecular weight of 235 kDa (6). Primary features of the TET1 protein include an N-terminal CXXC domain responsible for binding CpG islands in DNA, and a C-terminal catalytic domain containing a Cys-rich region followed by a double-stranded beta-helix (DSBH) domain that contributes to the mC dioxygenase activity and metal binding (1,2,4,5).

Recent studies have shown a role for TET1 in mediating epigenetic changes, such as DNA methylation status, in response to environmental factors such as food and nutrition, exercise, radiation, and allergens (3). The balance between DNA methylation and demethylation is crucial in health and homeostasis (3,5). In addition to response to environmental exposures, TET1 also plays a role in different diseases and cancer subtypes where it can function as either a tumor suppressor or tumor promoter (2,5). TET1 was originally identified as a fusion partner for the mixed lineage leukemia (MLL) gene in acute myeloid leukemia (AML) (1-3). While TET1 expression is low in AML, it is highly expressed in T-cell acute lymphoblastic leukemia (T-ALL) (2). Similarly, TET1 expression is suppressed in hormone receptor positive breast cancer (HRBC) but elevated in triple negative breast cancer (2). TET1 is a potential therapeutic target in certain cancers, but due to its complex role in different signaling pathways its potential needs to be more widely studied (2). While TET1 is primarily responsible for initiating DNA demethylation, it also functions alongside 5hmc in maintaining pluripotency in embryonic stem cells (ESCs) and can serve as a marker for differentiation (1,2,4,5).

References

1. Tan L, Shi YG. Tet family proteins and 5-hydroxymethylcytosine in development and disease. Development. 2012;139(11):1895-1902. https://doi.org/10.1242/dev.070771

2. Liu W, Wu G, Xiong F, Chen Y. Advances in the DNA methylation hydroxylase TET1. Biomark Res. 2021;9(1):76. https://doi.org/10.1186/s40364-021-00331-7

3. Zhu T, Brown AP, Ji H. The Emerging Role of Ten-Eleven Translocation 1 in Epigenetic Responses to Environmental Exposures. Epigenet Insights. 2020;13:2516865720910155. https://doi.org/10.1177/2516865720910155

4. Melamed P, Yosefzon Y, David C, Tsukerman A, Pnueli L. Tet Enzymes, Variants, and Differential Effects on Function. Front Cell Dev Biol. 2018;6:22. https://doi.org/10.3389/fcell.2018.00022

5. Ma C, Seong H, Liu Y, Yu X, Xu S, Li Y. Ten-eleven translocation proteins (TETs): tumor suppressors or tumor enhancers?. Front Biosci (Landmark Ed). 2021;26(10):895-915. https://doi.org/10.52586/4996

6. Uniprot (Q8NFU7)

Long Name

Methylcytosine dioxygenase TET1

Alternate Names

CXXC6, EC 1.14.11.n2, KIAA1676, LCX

Gene Symbol

TET1

UniProt

Q8NFU7 (Human)

Additional TET1 Products

Product Documents for TET1 Antibody - BSA Free

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Product Specific Notices for TET1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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