TET3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-18691

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
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Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against TET3 (Tet Methylcytosine Dioxygenase 3), using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for TET3 Antibody - BSA Free

Western Blot: TET3 Antibody [NBP3-18691]

Western Blot: TET3 Antibody [NBP3-18691]

Western Blot: TET3 Antibody [NBP3-18691] - Figure 3. Western blot analysis using the antibody directed against TET3 Whole cell extracts (25 ug) from Jurkat cells were analysed by Western blot using the antibody against TET3 diluted 1:200 in TBS- Tween containing 5% skimmed milk. Lane 2 shows the results after incubation of the antibody with the immunizing peptides. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Western Blot: TET3 Antibody [NBP3-18691]

Western Blot: TET3 Antibody [NBP3-18691]

Western Blot: TET3 Antibody [NBP3-18691] - Figure 2. Western blot analysis using the antibody directed against TET3 Whole cell extracts (25 ug) from Jurkat cells were analysed by Western blot using the antibody against TET3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
ELISA: TET3 Antibody [NBP3-18691]

ELISA: TET3 Antibody [NBP3-18691]

ELISA: TET3 Antibody [NBP3-18691] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against mouse TET3. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:20,300.

Applications for TET3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

1 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: TET3

TET3 may catalyze the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC)

Alternate Names

EC 1.14.11.n2, hCG_40738, KIAA0401, methylcytosine dioxygenase TET3, MGC22014, probable methylcytosine dioxygenase TET3, tet oncogene family member 3

Gene Symbol

TET3

UniProt

Additional TET3 Products

Product Documents for TET3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TET3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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