Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1018746
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Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein
Ala279-Ser390
Accession # P01137

Specificity

Detects human TGF-beta 1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TGF-beta 1 Antibody (1018746) [Alexa Fluor® 532]

TGF-beta 1 Antibody (1018746) [Alexa Fluor® 532]

TGF-beta 1 Antibody (1018746) [Alexa Fluor® 532] [FAB10502X] -

TGF-beta 1 Antibody (1018746) [Alexa Fluor® 532] [FAB10502X] - Vial of Alexa Fluor 532 conjugated antibody. Alexa Fluor 532 is optimally excited at 532 nm by the Yellow-Green laser (561 nm) and has an emission maximum of 554 nm.

Applications for TGF-beta 1 Antibody (1018746) [Alexa Fluor® 532]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TGF-beta 1

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Additional TGF-beta 1 Products

Product Documents for TGF-beta 1 Antibody (1018746) [Alexa Fluor® 532]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TGF-beta 1 Antibody (1018746) [Alexa Fluor® 532]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for TGF-beta 1 Antibody (1018746) [Alexa Fluor® 532]

Showing  1 - 2 of 2 FAQs Showing All
  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

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