hh TGF‑ beta 1/1.2 Antibody (AF-101-NA): R&D Systems

TGF-beta 1/1.2 Antibody

(21 citations)
(1 Review)
  
  • Specificity
    Detects TGF-beta 1/1.2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with TGF-beta 2 is observed.
  • Source
    Polyclonal Chicken IgY
  • Purification
    Antigen Affinity-purified from egg yolks
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1
    Ala270-Ser390
    Accession # P01137
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human TGF-beta 1 (Catalog # 240-B)
    Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)
  • Neutralization
    Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 5-30 ng/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by
TGF‑ beta 1/1.2 Antibody.
Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-TGF‑ beta 1/1.2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-101-NA). The ND50 is typically 5-30 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 1/1.2

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

  • Long Name:
    Transforming Growth Factor beta 1 and 1.2
  • Entrez Gene IDs:
    7040 (Human); 21803 (Mouse); 59086 (Rat)
  • Alternate Names:
    TGFb1.2; TGF-beta 1/1.2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment.
    Authors: Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J
    Sci Rep, 2015;5(0):8919.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014;111(21):E2229-36.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: IP
  3. Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.
    Authors: Kang I, Yoon D, Braun K, Wight T
    J Biol Chem, 2014;289(22):15393-404.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  4. IL-21 induces IL-22 production in CD4+ T cells.
    Authors: Yeste A, Mascanfroni I, Nadeau M, Burns E, Tukpah A, Santiago A, Wu C, Patel B, Kumar D, Quintana F
    Nat Commun, 2014;5(0):3753.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  5. The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-beta1 production and FcRL4 expression.
    Authors: Jelicic K, Cimbro R, Nawaz F, Huang D, Zheng X, Yang J, Lempicki R, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Okwara N, Wei D, Roby G, David A, Hwang I, Kehrl J, Arthos J, Cicala C, Fauci A
    Nat Immunol, 2013;14(12):1256-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  6. Long-term gene therapy with thrombospondin 2 inhibits TGF-beta activation, inflammation and angiogenesis in chronic allograft nephropathy.
    Authors: Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C
    PLoS ONE, 2013;8(12):e83846.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  7. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
    Authors: Oida T, Weiner HL
    J. Immunol., 2012;185(6):3529-35.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Homeostatic role of transforming growth factor-beta in the oral cavity and esophagus of mice and its expression by mast cells in these tissues.
    Authors: Vitsky A, Waire J, Pawliuk R, Bond A, Matthews D, Lacasse E, Hawes ML, Nelson C, Richards S, Piepenhagen PA, Garman RD, Andrews L, Thurberg BL, Lonning S, Ledbetter S, Ruzek MC
    Am. J. Pathol., 2009;174(6):2137-49.
    Species: Mouse
    Sample Type: Saliva
    Application: WB
  9. TGF-beta promotes thyroid epithelial cell hyperplasia and fibrosis in IFN-gamma-deficient NOD.H-2h4 mice.
    Authors: Yu S, Sharp GC, Braley-Mullen H
    J. Immunol., 2008;181(3):2238-45.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production.
    Authors: Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L
    Am. J. Pathol., 2008;172(4):1127-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. The LTK63 adjuvant improves protection conferred by Ag85B DNA-protein prime-boosting vaccination against Mycobacterium tuberculosis infection by dampening IFN-gamma response.
    Authors: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Fattorini L, Del Giudice G, Cassone A
    Vaccine, 2008;26(33):4237-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  12. Characterization of in vitro antimurine thymocyte globulin-induced regulatory T cells that inhibit graft-versus-host disease in vivo.
    Authors: Ruzek MC, Waire JS, Hopkins D, Lacorcia G, Sullivan J, Roberts BL, Richards SM, Nahill SR, Williams JM, Scaria A, Dzuris J, Shankara S, Garman RD
    Blood, 2007;111(3):1726-34.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  13. Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure.
    Authors: Neurohr C, Nishimura SL, Sheppard D
    Am. J. Respir. Cell Mol. Biol., 2006;35(2):252-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells.
    Authors: Dasgupta S, Bhattacharya-Chatterjee M, O&amp;amp;apos;Malley BW, Chatterjee SK
    J. Cell. Biochem., 2006;97(5):1036-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  15. Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
    Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
    Eur J Vasc Endovasc Surg, 2006;31(5):516-22.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  16. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  17. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005;20(4):790-6.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  18. Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice.
    Authors: Mesples B, Fontaine RH, Lelievre V, Launay JM, Gressens P
    Neurobiol. Dis., 2005;18(1):193-205.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  19. Autoreactive T cells persist in rats protected against experimental autoimmune encephalomyelitis and can be activated through stimulation of innate immunity.
    Authors: Conant SB, Swanborg RH
    J. Immunol., 2004;172(9):5322-8.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  20. Effect of neutralizing transforming growth factor beta1 on the immune response against Mycobacterium tuberculosis in guinea pigs.
    Authors: Allen SS, Cassone L, Lasco TM
    Infect. Immun., 2004;72(3):1358-63.
    Species: Guinea Pig
    Sample Type: In Vivo
    Application: In vivo
  21. Inhibition of neointimal hyperplasia after balloon injury by cis-element 'decoy' of early growth response gene-1 in hypercholesterolemic rabbits.
    Authors: Ohtani K, Egashira K, Usui M, Ishibashi M, Hiasa KI, Zhao Q, Aoki M, Kaneda Y, Morishita R, Takeshita A
    Gene Ther., 2004;11(2):126-32.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC Fresh
Expand to show all 21 Citations

FAQs

  1. Does AF-101-NA detect other isoforms of TGF-beta?

    • Cross-reactivity testing results are listed in the Specificity section on the Product Datasheet: less than 15% cross-reactivity with recombinant amphibian TGF-beta 5 and less than 2% with TGF-beta 2 and TGF-beta 3, have been observed, as measured by direct ELISA.

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Isotype Controls
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Block/Neutralize TGF-beta 1/1.2 Antibody AF-101-NA
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 Block/Neutralize Human Anonymous 06/28/2017
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Block/Neutralize TGF-beta 1/1.2 Antibody AF-101-NA
Block/Neutralize: TGF-beta 1/1.2 Antibody [AF-101-NA]

Summary

ApplicationBlock/Neutralize
Sample TestedMRC-5 human embryonic lung fibroblast cell line
SpeciesHuman

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