TGF‑ beta 1 Antibody

Name: TGF‑ beta 1 Antibody
Brand: R&D Systems
SKU: MAB2401
Price: 479 USD
Availability: InStock

R&D Systems | Catalog # MAB2401

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Citations (16)

Product Documents

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Rat, Porcine, Primate - Pan troglodytes (Chimpanzee)

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Neutralization, Neutralization Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 141322

View all TGF-beta 1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1

Specificity

Detects multiple species of TGF-beta 1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ alpha, rhTGF‑ beta 2, rhTGF‑ beta 3, rhTGF‑ beta 5, rhLAP (TGF‑ beta 1), rhART, rhNeurturin, or recombinant mouse TRANCE.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for TGF‑ beta 1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta 1 (Catalog # 240-B)
under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TGF-beta 1

TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.