TGF‑ beta 1 Biotinylated Antibody

R&D Systems | Catalog # BAF240

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Rat, Porcine, Bovine, Primate - Papio anubis (Olive Baboon)

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, Neutralization, Flow Cytometry, Immunoprecipitation, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Polyclonal Chicken IgY
View all TGF-beta 1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1 (R&D Systems, Catalog # 240-B)

Specificity

Detects human TGF-beta 1 in ELISAs and Western blots. In sandwich immunoassays, when used in combination with the rhTGF‑ beta  sRII/Fc chimera (Catalog # 341-BR), approximately 15% cross-reactivity with recombinant human (rh) Latent TGF‑ beta 1 is observed, 1% cross-reactivity with rhTGF-beta 1.2 is observed, and 3% cross-reactivity with recombinant amphibian (ra) TGF-beta 5 is observed. When used in combination with the monoclonal capture antibody (Catalog # MAB2402), approximately 5% cross‑reactivity with TGF-beta 1.2 is observed and 1% cross-reactivity with rhTGF-beta 2, recombinant chicken TGF-beta 3, and raTGF-beta 5 is observed.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Applications for TGF‑ beta 1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TGF-beta 1 (Catalog # 240-B)

Human TGF-beta 1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Recombinant Human TGF‑ beta  RII Fc Chimera (Catalog # 341-BR)
  • Capture Reagent: TGF-beta 1, 2, 3 Antibody (Catalog # MAB1835)
  • Capture Reagent: TGF‑ beta 1 Antibody (Catalog # MAB2402)
  • Standard: Recombinant Human TGF-beta 1 Protein (Catalog # 240-B)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.8 using BAF240 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from egg yolks

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 1

TGF- beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1 - 7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1 - 4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8 - 10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity (1). TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

 

References

  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for TGF‑ beta 1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for TGF‑ beta 1 Biotinylated Antibody

For research use only

Citations for TGF‑ beta 1 Biotinylated Antibody

Customer Reviews for TGF‑ beta 1 Biotinylated Antibody (4)

4.8 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • TGF-beta 1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 03/15/2021
    Dose response of TGFb1 and TGFb3, detection with Anti-TGFb1-biotin. Anti-TGFb1-biotin is specific for TGFb1 and no signal for TGFb3
    TGF‑ beta 1 Biotinylated Antibody BAF240
  • TGF-beta 1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/29/2019
    TGF‑ beta 1 Biotinylated Antibody BAF240
  • TGF-beta 1 Biotinylated Antibody
    Name: Anonymous
    Application: competition ELISA
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 03/28/2019
    I coated my plate with 50ng/well of recombinant mouse TGF beta 1 protein (cat# 7666-MB) overnight. I washed and blocked with reagent diluent 2 DY995 for 2hr, washed and added another company's TGFb1 antibody at different concentrations 0.2-100ug/mL in reagent diluent 2 and incubated 1hr. I did not wash my wells but add the two antibodies MAB240-biotinylated it in-house and BAF at a final concentration of 2ug/mL and incubated 1hr diluted in reagent diluent 2. I washed and added streptavidin-HRP DY998 (1:200) and incubated 30 mins, washed and developed with the substrate pack DY999. I found MAB240 competed for binding to the mouse TGF beta 1 protein when the competitor's antibody was present but the chicken polyclonal was not inhibited.
    TGF‑ beta 1 Biotinylated Antibody BAF240
  • TGF-beta 1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 01/05/2018
    This antibody was used as the detection antibody with MAB420 as the capture in an ELISA for TGF-beta 1.
    TGF‑ beta 1 Biotinylated Antibody BAF240

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