TGF-beta 1 Biotinylated Antibody
TGF-beta 1 Biotinylated Antibody Summary
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Applications
Human TGF-beta 1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 1
TGF- beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1 - 7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1 - 4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8 - 10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity (1). TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).
- Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
- Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
- Wahl, S.M. (2006) Immunol. Rev. 213:213.
- Chang, H. et al. (2002) Endocr. Rev. 23:787.
- Lin, J.S. et al. (2006) Reproduction 132:179.
- Hinck, A.P. et al. (1996) Biochemistry 35:8517.
- Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
- Derynck, R. et al. (1985) Nature 316:701.
- Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
- Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
- de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
- Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Product Datasheets
Citations for TGF-beta 1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cancer immunotherapy via targeted TGF-&beta signalling blockade in TH cells
Authors: S Li, M Liu, MH Do, C Chou, EG Stamatiade, BG Nixon, W Shi, X Zhang, P Li, S Gao, KJ Capistrano, H Xu, NV Cheung, MO Li
Nature, 2020;0(0):.
Species: Mouse
Sample Types: Reference Standard
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Circulating Picomolar Levels of CCL2 Downregulate Ongoing Chronic Experimental Autoimmune Encephalomyelitis by Induction of Regulatory Mechanisms
Authors: N Kaushansky, E Bakos, S Becker-Her, I Shachar, A Ben-Nun
J. Immunol., 2019;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Detection -
TGF??1 upregulates the expression of lncRNA?ATB to promote atherosclerosis
Authors: H Yu, S Ma, L Sun, J Gao, C Zhao
Mol Med Rep, 2019;19(5):4222-4228.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Detection) -
Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGF? enhance the efficacy of cancer immunotherapy
Authors: R Ravi, KA Noonan, V Pham, R Bedi, A Zhavoronko, IV Ozerov, E Makarev, A V Artemov, PT Wysocki, R Mehra, S Nimmagadda, L Marchionni, D Sidransky, IM Borrello, E Izumchenko, A Bedi
Nat Commun, 2018;9(1):741.
Applications: ELISA Development (Detection) -
Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
Nat Commun, 2015;6(0):6353.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Induction and activation of latent transforming growth factor-beta1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1).
Authors: Ballesteros A, Mentink-Kane M, Warren J, Kaplan G, Dveksler G
J Biol Chem, 2015;290(7):4422-31.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Role of a Novel Human Leukocyte Antigen-DQA1*01:02;DRB1*15:01 Mixed Isotype Heterodimer in the Pathogenesis of "Humanized" Multiple Sclerosis-like Disease.
Authors: Kaushansky N, Eisenstein M, Boura-Halfon S, Hansen B, Nielsen C, Milo R, Zeilig G, Lassmann H, Altmann D, Ben-Nun A
J Biol Chem, 2015;290(24):15260-78.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
J Immunol, 2014;192(2):804-16.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
Diabetologia, 2011;54(10):2713-23.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Activation of Toll-like receptor 3 augments myofibroblast differentiation.
Authors: Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M
Am. J. Respir. Cell Mol. Biol., 2009;40(6):654-62.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid.
Authors: Saurer L, McCullough KC, Summerfield A
J. Immunol., 2007;179(6):3504-14.
Species: Porcine
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Antigen-induced regulatory T cells in HBV chronically infected patients.
Authors: Barboza L, Salmen S, Goncalves L, Colmenares M, Peterson D, Montes H, Cartagirone R, Gutierrez Mdel C, Berrueta L
Virology, 2007;368(1):41-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
Immunol. Lett., 2006;105(2):159-66.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004;80(6):1521-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3.
Authors: Chen, WanJun, Jin, Wenwen, Hardegen, Neil, Lei, Ke-Jian, Li, Li, Marinos, Nancy, McGrady, George, Wahl, Sharon M
J Exp Med, 2003;198(12):1875-86.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
Am. J. Physiol. Lung Cell Mol. Physiol., 2002;282(5):L1049-56.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
J. Immunol., 2000;164(10):5337-43.
Species: Primate - Papio anubis (Olive Baboon)
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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Reviews for TGF-beta 1 Biotinylated Antibody
Average Rating: 4.8 (Based on 4 Reviews)
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Dose response of TGFb1 and TGFb3, detection with Anti-TGFb1-biotin. Anti-TGFb1-biotin is specific for TGFb1 and no signal for TGFb3
I coated my plate with 50ng/well of recombinant mouse TGF beta 1 protein (cat# 7666-MB) overnight. I washed and blocked with reagent diluent 2 DY995 for 2hr, washed and added another company's TGFb1 antibody at different concentrations 0.2-100ug/mL in reagent diluent 2 and incubated 1hr. I did not wash my wells but add the two antibodies MAB240-biotinylated it in-house and BAF at a final concentration of 2ug/mL and incubated 1hr diluted in reagent diluent 2. I washed and added streptavidin-HRP DY998 (1:200) and incubated 30 mins, washed and developed with the substrate pack DY999. I found MAB240 competed for binding to the mouse TGF beta 1 protein when the competitor's antibody was present but the chicken polyclonal was not inhibited.
This antibody was used as the detection antibody with MAB420 as the capture in an ELISA for TGF-beta 1.
