TIM-4 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-80837
![Western Blot: TIM-4 AntibodyBSA Free [NB100-80837]](https://resources.rndsystems.com/images/products/TIM-4-Antibody---BSA-Free-Western-Blot-NB100-80837-img0007.jpg "Western Blot: TIM-4 AntibodyBSA Free [NB100-80837]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A 16 amino acid synthetic peptide near the center of human TIM-4. The immunogen is located within amino acids 60 - 110 of TIM-4.
Epitope
Internal
Specificity
At least four isoforms of TIM-4 are known to exist; this antibody will detect all four isoforms.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TIM-4 Antibody - BSA Free
Western Blot: TIM-4 AntibodyBSA Free [NB100-80837]
Western Blot: TIM-4 Antibody - BSA Free [NB100-80837] - Jurkat lysate with TIM-4 antibody at (A) 1 and (B) 2 ug/ml.![Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837]](https://resources.rndsystems.com/images/products/TIM-4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-80837-img0004.jpg "Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837]")
Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837]
Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.![Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837]](https://resources.rndsystems.com/images/products/TIM-4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-80837-img0005.jpg "Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837]")
Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837]
Immunocytochemistry/Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837] - Jurkat cells with TIM-4 antibody at 10 ug/ml.![Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837]](https://resources.rndsystems.com/images/products/TIM-4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-80837-img0006.jpg "Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837]")
Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837]
Immunocytochemistry/Immunofluorescence: TIM-4 Antibody - BSA Free [NB100-80837] - Jurkat cells with TIM-4 antibody at 20 ug/ml.![Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837]](https://resources.rndsystems.com/images/products/TIM-4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-80837-img0003.jpg "Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837]")
Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837]
Immunohistochemistry-Paraffin: TIM-4 Antibody - BSA Free [NB100-80837] - Staining of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.Applications for TIM-4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Frozen
1 - 2 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TIM-4
Long Name
T Cell Immunoglobulin Mucin-4
Alternate Names
Smuckler, TIM4, TIMD4
Gene Symbol
TIMD4
UniProt
Additional TIM-4 Products
Product Documents for TIM-4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TIM-4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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