Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TIM-4 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-4. The immunogen is located within amino acids 60 - 110 of TIM-4. Amino Acid Squence: RTDGMRVTSRKSAKYR

Specificity

At least four isoforms of TIM-4 are known to exist; this antibody will detect all four isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TIM-4 Antibody - BSA Free (NBP1-76702) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TIM-4 Antibody - BSA Free

Western Blot: TIM-4 AntibodyBSA Free [NBP1-76702]

Western Blot: TIM-4 AntibodyBSA Free [NBP1-76702]

Western Blot: TIM-4 Antibody [NBP1-76702] - Analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 ug/mL in (A) the absence and (B) the presence of blocking peptide.
TIM-4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] -

Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 ug/mL.
TIM-4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] -

Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 ug/mL.
TIM-4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] -

Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 ug/ml.

Green: TIM-4 Antibody
Blue: DAPI staining
TIM-4 Antibody - BSA Free

Immunohistochemistry: TIM-4 Antibody - BSA Free [NBP1-76702] -

Immunohistochemistry: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 ug/ml.
TIM-4 Antibody - BSA Free

Immunohistochemistry: TIM-4 Antibody - BSA Free [NBP1-76702] -

Immunohistochemistry: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunohistochemistry of TIM-4 in mouse liver tissue with TIM-4 antibody at 2 ug/ml.

Applications for TIM-4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIM-4

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.

Long Name

T Cell Immunoglobulin Mucin-4

Alternate Names

Smuckler, TIM4, TIMD4

Entrez Gene IDs

91937 (Human); 276891 (Mouse)

Gene Symbol

TIMD4

UniProt

Q96H15

Additional TIM-4 Products

Product Documents for TIM-4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TIM-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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