TLR1 Antibody - BSA Free

Name: TLR1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB100-56563
Price: 179 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-56563

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Citations (16)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Dot Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Cytometric Bead Assay Standard, Flow Cytometry Control, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all TLR1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a KLH-conjugated synthetic peptide corresponding to a sequence within amino acids 400-450 of human TLR1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TLR1 Antibody - BSA Free

Flow Cytometry: TLR1 Antibody [NB100-56563]

TLR1-Antibody-Flow-Cytometry-NB100-56563-img0016.jpg

Western Blot: TLR1 Antibody [NB100-56563]

Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.

Western Blot: TLR1 Antibody [NB100-56563]

TLR1-Antibody-Western-Blot-NB100-56563-img0014.jpg

Flow Cytometry: TLR1 Antibody [NB100-56563]

TLR1-Antibody-Flow-Cytometry-NB100-56563-img0015.jpg

Immunohistochemistry-Paraffin: TLR1 Antibody [NB100-56563]

Immunohistochemistry-Paraffin: TLR1 Antibody [NB100-56563] - analysisof human small intestine using this antibody at 10 ug/ml.

Flow Cytometry: TLR1 Antibody [NB100-56563]

Flow Cytometry: TLR1 Antibody [NB100-56563] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56563. Staining of TLR1 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells alone, green represents isotype controlwas used for this test.

Flow Cytometry: TLR1 Antibody [NB100-56563]

Flow Cytometry: TLR1 Antibody [NB100-56563] - Intracellular analysis of TLR1 AF647 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells without antibody, green represents isotype control, red represents TLR1 antibody.

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Applications for TLR1 Antibody - BSA Free

Application

Recommended Usage

Flow (Cell Surface)

reported in scientific literature (Wong et al (2007); PMID 23593278)

Flow (Intracellular)

0.5-1 ug/1 million cells

Flow Cytometry

2-4 ug/ 1x10-6 cells

Immunohistochemistry

reported in scientific literature (PMID 27008696)

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-3 ug/ml

Application Notes

In Ramos, an 87 kDa band is observed. Flow (Intracellular) reported in scientific literature (PMID: 23593278)

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR1

The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. The TLRs could constitute an important and unrecognized component of innate immunity in humans. In mice, TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide. They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Long Name

Toll-like Receptor 1

Alternate Names

CD281

Entrez Gene IDs

7096 (Human); 305354 (Rat)

Gene Symbol

TLR1

UniProt

NP_003254

Additional TLR1 Products

Product Documents for TLR1 Antibody - BSA Free

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Product Specific Notices for TLR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.