TLR2 Antibody (67N8F8)

Name: TLR2 Antibody (67N8F8)
Brand: Novus Biologicals
SKU: NBP2-25245
Price: 169 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-25245

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Citations (1)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface)

Cited:

Flow (Cell Surface)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 67N8F8

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Product Specifications

Immunogen

Amino acids 227-371 of human TLR2 were used as the immunogen for the antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Novus Biologicals Mouse TLR2 Antibody (67N8F8) (NBP2-25245) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-TLR2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TLR2 Antibody (67N8F8)

Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245]

Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of human lymph node tissue using TLR2 antibody at a concentration of 1 ug/ml.

Immunohistochemistry-Paraffin: TLR2 Antibody (67N8F8) [NBP2-25245]

Immunohistochemistry-Paraffin: TLR2 Antibody (67N8F8) [NBP2-25245] - FFPE human esophageal squamous cell carcinoma stained with TLR2 antibody at 5 ug/ml.

Flow (Cell Surface): TLR2 Antibody (67N8F8) [NBP2-25245]

Flow (Cell Surface): TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis using the FITC conjugate of NBP2-25259. Surface staining of stable HEK293/hTLR2 cells (red) and vector control cells (green) using TLR2 antibody at 5 ul/10^6 cells. Image using the Azide Free form of this antibody.

Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245]

Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of (A) TLR2 partial recombinant protein, (B) transfected cell lysate, and (C) control 293T lysate using TLR2 antibody at 1 ug/ml.

Flow Cytometry: TLR2 Antibody (67N8F8) [NBP2-25245]

Flow Cytometry: TLR2 Antibody (67N8F8) [NBP2-25245] - (A) Surface staining of transfected TLR2 HEK293 cells (red) and mock transfected cells (green) using TLR2 antibody at 1 ug/10^6 cells. FITC-conjugated anti-mouse IgG secondary was used for this test. (B) Surface staining of PPI-negative PBMC (monocyte-gated) using TLR2 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells. PE-conjugated anti-mouse IgG secondary was used for this test.

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Applications for TLR2 Antibody (67N8F8)

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-3 ug/ml

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR2

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-B.[2] Recent research indicates that in the intestine, TLR2 functionality is essential for regulating the expression of CYP1A1, which is a key enzyme in detoxication of carcinogenic polycyclic aromatic hydrocarbons such as benzo(a)pyrene.

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

UniProt

NP_003255.2 (Human)

Additional TLR2 Products

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Product Specific Notices for TLR2 Antibody (67N8F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.