TLR2 Antibody (67N8F8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-25259
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 67N8F8
Format
Azide and BSA Free
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Product Specifications
Immunogen
Amino acids 227-371 of human TLR2 were used as the immunogen for the antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TLR2 Antibody (67N8F8) - Azide and BSA Free
Western Blot: TLR2 Antibody (67N8F8) [Azide Free] [NBP2-25259] - Analysis of TLR2 partial recombinant protein (A), transfected cell lysate (B), and control 293T lysate (C) using TLR2 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: TLR2 Antibody (67N8F8) [Azide Free] [NBP2-25259] - Formalin-fixed, paraffin-embedded human esophageal squamous cell carcinoma stained with TLR2 antibody at 5 ug/ml.
Flow Cytometry: TLR2 Antibody (67N8F8) [Azide Free] [NBP2-25259] - Analysis using the FITC conjugate of NBP2-25259. Surface staining of stable HEK293/hTLR2 cells (red) and vector control cells (green) using TLR2 antibody at 5 ul/10^6 cells.
Flow Cytometry: TLR2 Antibody (67N8F8) [Azide Free] [NBP2-25259] - A. Surface staining of transfected TLR2 HEK293 cells (red) and mock transfected cells (green) using TLR2 antibody at 1 ug/10^6 cells. FITC-conjugated anti-mouse IgG secondary was used for this test. B. Surface staining of PPI-negative PBMC (monocyte-gated) using TLR2 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells. PE-conjugated anti-mouse IgG secondary was used for this test.
Flow Cytometry: TLR2 Antibody (67N8F8) [Azide Free] [NBP2-25259] - Surface staining of stable HEK293/hTLR2 cells (red) and vector control cells ( green) using TLR2 AF647 antibody at 5 ul/10^6 cells.
Applications for TLR2 Antibody (67N8F8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1ul/1 million cells
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-3 ug/ml
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Sterile - filtered PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TLR2
Long Name
Toll-like Receptor 2
Alternate Names
CD282
Gene Symbol
TLR2
Additional TLR2 Products
Product Documents for TLR2 Antibody (67N8F8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TLR2 Antibody (67N8F8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
