Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Drosophila, Goat, Hamster, Monkey, Rabbit, Sheep, Xenopus, Yeast

Cited:

Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TNF RI/TNFRSF1A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to a portion of mouse TNF-R1. The sequence is completely conserved in rat and human.

Reactivity Notes

Yeast TNF-R1. Mouse and Goat reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TNF RI/TNFRSF1A Antibody - BSA Free

Western Blot: TNF RI/TNFRSF1A Antibody [NBP1-97453]

Western Blot: TNF RI/TNFRSF1A Antibody [NBP1-97453]

Western Blot: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of TNF-R1: Lane 1: MW marker, Lane 2: Hela (HS), Lane 3: Jurkat.
Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP1-97453]

Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP1-97453]

Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of human spleen tissue stained with TNF receptor 1 pAb at 10ug/ml.
Flow Cytometry: TNF RI/TNFRSF1A Antibody [NBP1-97453]

Flow Cytometry: TNF RI/TNFRSF1A Antibody [NBP1-97453]

Flow Cytometry: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF Receptor I Antibody [NBP1-97453] - MLL-AF9 transduced murine leukemia cells. Tnfr-/- cells were used as negative control. Image provided by verified customer.
TNF RI/TNFRSF1A Antibody - BSA Free

Western Blot: Rabbit Polyclonal TNF RI/TNFRSF1A Antibody [NBP1-97453]

Western Blot: Rabbit Polyclonal TNF RI/TNFRSF1A Antibody [NBP1-97453] -Quantification of TNF alpha in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. NBP1-97453 was used at a dilution of 1:1000 and Beta-actin antibody (Catalog # NB600-501) was used at a dilution of 1:3000. Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 10 days after graft implantation. Image from a verified customer review.

Applications for TNF RI/TNFRSF1A Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:20

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

12.5 ug/ml

Western Blot

1:1000
Application Notes
TNF RI/TNFRSF1A antibody validated for FLOW from a verified customer review.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-97453 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TNF RI/TNFRSF1A

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.

Long Name

Tumor Necrosis Factor Receptor I

Alternate Names

CD120a, TNFRI, TNFRSF1A

Entrez Gene IDs

7132 (Human)

Gene Symbol

TNFRSF1A

Additional TNF RI/TNFRSF1A Products

Product Documents for TNF RI/TNFRSF1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TNF RI/TNFRSF1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TNF RI/TNFRSF1A Antibody - BSA Free

Customer Reviews for TNF RI/TNFRSF1A Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • TNF RI/TNFRSF1A Antibody - BSA Free
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone and Bone Extracts
    Species: Goat
    Verified Customer | Posted 05/13/2025
    Quantification of TNF alpha in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. NBP1-97453 was used at a dilution of 1:1000.
    Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 10 days after graft implantation.
    TNF RI/TNFRSF1A Antibody - BSA Free NBP1-97453
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Name: Andrew Volk
    Application: Flow Cytometry
    Sample Tested: Mouse MLL-AF9 transduced leukemia cells
    Species: Mouse
    Verified Customer | Posted 01/31/2014
    MLL-AF9 transduced murine leukemia cells. Tnfr-/- cells were used as negative control
    TNF RI/TNFRSF1A Antibody - BSA Free NBP1-97453

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