![Western Blot: TNIK Antibody [NBP3-13022]](https://resources.rndsystems.com/images/products/TNIK-Antibody-Western-Blot-NBP3-13022-img0005.jpg "Western Blot: TNIK Antibody [NBP3-13022]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for TNIK Antibody - BSA Free
Western Blot: TNIK Antibody [NBP3-13022]
Western Blot: TNIK Antibody [NBP3-13022] - TNIK antibody validation by shRNA knock-down Upper panel: TNIK antibody (NBP3-13022) Lower panel: p84 antibody(NB100-174) A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of TNIK shRNA-transfected 293T cells 5% SDS-PAGE TNIK antibody (NBP3-13022) dilution: 1:5000 p84 antibody(NB100-174) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]](https://resources.rndsystems.com/images/products/TNIK-Antibody-Immunohistochemistry-Paraffin-NBP3-13022-img0004.jpg "Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]")
Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]
Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytosol on Mouse small intestine by immunohistochemical analysis. Sample: Paraffin-embedded Mouse small intestine TNIK antibody (NBP3-13022) dilution: 1:100 Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min![Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]](https://resources.rndsystems.com/images/products/TNIK-Antibody-Immunohistochemistry-Paraffin-NBP3-13022-img0002.jpg "Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]")
Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]
Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytosol on mouse Colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse Colon TNIK antibody (NBP3-13022) dilution: 1:100 Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min![Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]](https://resources.rndsystems.com/images/products/TNIK-Antibody-Immunohistochemistry-Paraffin-NBP3-13022-img0003.jpg "Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]")
Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]
Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytosol on Mouse Kidney by immunohistochemical analysis. Sample: Paraffin-embedded Mouse Kidney TNIK antibody (NBP3-13022) dilution: 1:100 Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence: TNIK Antibody [NBP3-13022] -
TNIK antibody detects TNIK protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNIK protein stained by TNIK antibody (NBP3-13022) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.
Blue: Hoechst 33342 staining.
Applications for TNIK Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TNIK
Alternate Names
EC 2.7.11, KIAA0551EC 2.7.11.1, TRAF2 and NCK interacting kinase, TRAF2 and NCK-interacting protein kinase
Gene Symbol
TNIK
Additional TNIK Products
Product Documents for TNIK Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TNIK Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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