TOM70 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87863

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TOM70 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLLSTQDFNMAADIDPQNADVYHHRGQLKILLDQVEEAVADFDECIRLRPESALAQAQKCFALYRQAYTGNNSSQIQAAMKGFEEVIKKFPRCAEGYALYAQALTDQQQFGKADEMYDKCIDLEPDNATT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOM70 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TOM70 Antibody [NBP1-87863]

Immunocytochemistry/ Immunofluorescence: TOM70 Antibody [NBP1-87863]

Immunocytochemistry/Immunofluorescence: TOM70 Antibody [NBP1-87863] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863] - Staining of human Pancreas shows very weak granular cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863] - Staining of human Cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863] - Staining of human Cerebral cortex shows strong granular cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-87863] - Staining of human Testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Simple Western: TOM70 Antibody [NBP1-87863]

Simple Western: TOM70 Antibody [NBP1-87863]

Simple Western: TOM70 Antibody [NBP1-87863] - Detection of TOM70 in purified mitochondrial (lanes 1-3 at 1:100, 1:50 and 1:25 dilutions, respectively) and cytosol (lane 4 at 1:25 dilution) preparations of SVG-A cells. Image from verified customer review.
TOM70 Antibody - BSA Free Western Blot: TOM70 Antibody - BSA Free [NBP1-87863]

Western Blot: TOM70 Antibody - BSA Free [NBP1-87863]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
TOM70 Antibody - BSA Free Western Blot: TOM70 Antibody - BSA Free [NBP1-87863]

Western Blot: TOM70 Antibody - BSA Free [NBP1-87863]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
TOM70 Antibody - BSA Free Immunohistochemistry-Paraffin: TOM70 Antibody - BSA Free [NBP1-87863]

Immunohistochemistry-Paraffin: TOM70 Antibody - BSA Free [NBP1-87863]

Analysis in human cerebral cortex and pancreas tissues using HPA014589 antibody. Corresponding TOMM70 RNA-seq data are presented for the same tissues.

Applications for TOM70 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Simple Western

Verified by a customer review

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in Purified mitochondrial and cytosol preparations of SVG-A cells, separated by Size, antibody dilution of 1:100

Reviewed Applications

Read 1 review rated 4 using NBP1-87863 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TOM70

Functional mitochondria require up to 1000 proteins to function properly, with 99% synthesized as precursors in the cytoplasm and transported into the mitochondria with the aid of cytosolic chaperones and mitochondrial translocators (import components). Proteins to be imported are chaperoned to the mitochondria by the cytosolic heat shock protein (cHSP70) and are immediately pursued by Translocators of the Outer Membrane (TOMs), followed by transient interactions of the unfolded proteins with Translocators of the Inner Membrane (TIMs). TOMM70A is ubiquitously expressed in human tissues and localizes in the mitochondria. TOMM70A could play a significant role in the import of nuclear-encoded mitochondrial proteins with internal targeting sites such as ADP/ATP carriers and the uncoupling proteins.

Alternate Names

FLJ90470, KIAA0719, mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor, TOM70, Translocase of outer membrane 70 kDa subunit, translocase of outer mitochondrial membrane 70 (yeast) homolog A, translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae), translocase of outer mitochondrial membrane 70 homolog A (yeast)

Gene Symbol

TOMM70

Additional TOM70 Products

Product Documents for TOM70 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TOM70 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TOM70 Antibody - BSA Free

Customer Reviews for TOM70 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Simple Western
    Sample Tested: purified mitochondrial and cytosol preparations of SVG-A cells
    Species: Human
    Verified Customer | Posted 08/03/2016
    Detection of TOM70 in purified mitochondrial (lanes 1-3) and cytosol (lane 4) preparations of SVG-A cells
    TOM70 Antibody - BSA Free NBP1-87863

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