TOR/mTOR [p Ser2481] Antibody (3H11) - BSA Free

Name: TOR/mTOR [p Ser2481] Antibody (3H11) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26405

Novus Biologicals | Catalog # NBP3-26405

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Product Documents

Recombinant Monoclonal Antibody

Discontinued Product

NBP3-26405 has been discontinued. View all TOR products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3H11 expressed in HEK293

Format

BSA Free

View all TOR Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human TOR/mTOR [p Ser2481] [UniProt P42345]

Modification

p Ser 2481

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOR/mTOR [p Ser2481] Antibody (3H11) - BSA Free

Western Blot: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] -

Western Blot: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Positive Western Blot detected in A549 whole cell lysate, 293 whole cell lysate (treated with Calyculin A or EGF).
All lanes: TOR/mTOR [p Ser2481] Antibody at 1.33ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 289 KDa
Observed band size: 289 KDa

Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] -

Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Staining of Hela cells with TOR/mTOR [p Ser2481] Antibody (3H11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for TOR/mTOR [p Ser2481] Antibody (3H11) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TOR

The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase which helps to mediate many cellular processes. Specifically, mTOR is invloved in the cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex, making mTOR antibodies useful tools for a number of important research areas.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Gene Symbol

MTOR

Additional TOR Products

Product Documents for TOR/mTOR [p Ser2481] Antibody (3H11) - BSA Free

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Product Specific Notices for TOR/mTOR [p Ser2481] Antibody (3H11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.