TRAILR1/TNFRSF10A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-25092

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRAILR1/TNFRSF10A Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of human DR4 protein. The immunogen is located within the last 50 amino acids of DR4.

Specificity

Human TRAILR1/TNFRSF10A has only 1 isoform (468aa, 50kD). TRAILR1/TNFRSF10A antibody has no cross reaction to TRAILR2/TNFRSF10B.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRAILR1/TNFRSF10A Antibody - BSA Free

Western Blot: TRAILR1/TNFRSF10A AntibodyBSA Free [NBP2-25092]

Western Blot: TRAILR1/TNFRSF10A AntibodyBSA Free [NBP2-25092]

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Western Blot Validation in Cell Lines. Loading: 15 ug of cell lysates per lane. Antibodies: DR4 NBP2-25092 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry: TRAILR1/TNFRSF10A Antibody - BSA Free [NBP2-25092]

Immunohistochemistry: TRAILR1/TNFRSF10A Antibody - BSA Free [NBP2-25092]

Immunohistochemistry: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Immunofluorescence Validation of DR4. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling DR4 with NBP2-25092 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody (red) and DAPI staining (blue). Image showing membrane staining on human spleen cells.
Western Blot: TRAILR1/TNFRSF10A AntibodyBSA Free [NBP2-25092]

Western Blot: TRAILR1/TNFRSF10A AntibodyBSA Free [NBP2-25092]

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TRAILR1/TNFRSF10A KO cell lysates. Antibodies: TRAILR1/TNFRSF10A [NBP2-25092] (1 ug/ml) and beta-actin [NBP1-76692] (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: TRAILR1/TNFRSF10A AntibodyBSA Free [NBP2-25092]

Western Blot: TRAILR1/TNFRSF10A AntibodyBSA Free [NBP2-25092]

Western Blot: TRAILR1/TNFRSF10A Antibody [NBP2-25092] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: DR4 NBP2-25092 (1 ug/mL), DR4 NBP1-76473 ( 4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.
TRAILR1/TNFRSF10A Antibody - BSA Free

Immunohistochemistry: TRAILR1/TNFRSF10A Antibody - BSA Free [NBP2-25092] -

Immunohistochemistry: TRAILR1/TNFRSF10A Antibody - BSA Free [NBP2-25092] - Validation of TRAILR1/TNFRSF10A Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TRAILR1/TNFRSF10A antibody at 10 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4μC. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
TRAILR1/TNFRSF10A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRAILR1/TNFRSF10A Antibody - BSA Free [NBP2-25092] -

Immunocytochemistry/ Immunofluorescence: TRAILR1/TNFRSF10A Antibody - BSA Free [NBP2-25092] - Validation of TRAILR1/TNFRSF10A in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-TRAILR1/TNFRSF10A antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin

Applications for TRAILR1/TNFRSF10A Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/mL

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TRAILR1/TNFRSF10A

DR4 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL2, 3, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kB activation in many tissues and cells.

Long Name

TRAIL Receptor 1

Alternate Names

CD261, DR4, TNFRSF10A, TRAIL R1

Gene Symbol

TNFRSF10A

UniProt

AAC51226

Additional TRAILR1/TNFRSF10A Products

Product Documents for TRAILR1/TNFRSF10A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAILR1/TNFRSF10A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAILR1/TNFRSF10A Antibody - BSA Free

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Protocols

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