Transferrin Antibody (HTF-14) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-37718

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine, Bovine (Negative), Canine (Negative), Equine (Negative), Rabbit, Sheep (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Functional, Radioimmunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HTF-14

Format

Azide and BSA Free
View all Transferrin Primary Antibodies »

Product Specifications

Immunogen

Purified porcine transferrin.

Localization

Secreted

Specificity

The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein

Scientific Data Images for Transferrin Antibody (HTF-14) - Azide and BSA Free

Western Blot: Transferrin Antibody (HTF-14)Azide and BSA Free [NBP2-37718]

Western Blot: Transferrin Antibody (HTF-14)Azide and BSA Free [NBP2-37718]

Western Blot: Transferrin Antibody (HTF-14) - Azide Free [NBP2-37718] - Human transferrin detected by the antibody. 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ug/well (red. con.) M. Low Range marker 4. hTransferrin; 1 ug/well (non-red. con.) 5. hTransferrin; 3 ug/well (non-red. con.) 6. hTransferrin; 5 ug/well (non-red. con.)
Functional: Transferrin Antibody (HTF-14) - Azide and BSA Free [NBP2-37718]

Functional: Transferrin Antibody (HTF-14) - Azide and BSA Free [NBP2-37718]

Functional: Transferrin Antibody (HTF-14) - Azide Free [NBP2-37718] - Staining of human placenta (paraffin sections) using anti-transferrin (HTF-14).

Applications for Transferrin Antibody (HTF-14) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000RIA 1:100-1:2000Blocking/Neutralizing 50-200 molar excess

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Radioimmunoassay

1:100-1:2000

Western Blot

1-2 ug/ml
Application Notes
Western Blot: non-reducing conditions
Blocking/Neutralizing: this antibody blocks binding of transferrin to the receptor.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Transferrin

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Gene Symbol

TF

Additional Transferrin Products

Product Documents for Transferrin Antibody (HTF-14) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Transferrin Antibody (HTF-14) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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