Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20405

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Predicted:

Porcine (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin/TAGLN/SM22 alpha. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (81%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Coronary artery vascular smooth muscle cells in fixed, frozen adult mouse heart. Primary antibody 1:100 dilution of a 0.2mg/ml solution. This image was submitted via customer Review.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Western blot analysis of Transgelin and beta-actin (lower panel) Sample (35 ug of whole cell lysate)

A: A-10 cell
B: A-10 cell treated with MG132 NBP2-20405.
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Paraffin-embedded mouse esophagus. Transgelin antibody dilution: 1:500.
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - 40X image of coronary artery vascular smooth muscle stained for transgelin. Primary Antibody dilution of 1:100. This image was submitted via customer Review.
Transgelin/TAGLN/SM22 alpha Antibody

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin/TAGLN/SM22 alpha antibody (NBP2-20405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Transgelin/TAGLN/SM22 alpha Antibody

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Transgelin/TAGLN/SM22 alpha antibody detects Transgelin/TAGLN/SM22 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Transgelin/TAGLN/SM22 alpha stained by Transgelin/TAGLN/SM22 alpha antibody (NBP2-20405) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Transgelin/TAGLN/SM22 alpha Antibody

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -

Transgelin antibody detects Transgelin protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Transgelin antibody (NBP2-20405) dilution: 1:500.

Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -

Transgelin antibody detects Transgelin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin protein stained by Transgelin antibody (NBP2-20405) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Reviewed Applications

Read 2 reviews rated 3 using NBP2-20405 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Transgelin/TAGLN

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

SM22 alpha, TAGLN, WS3-10

Gene Symbol

TAGLN

UniProt

Additional Transgelin/TAGLN Products

Product Documents for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free (2)

3 out of 5
2 Customer Ratings
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Customer Images


Showing  1 - 2 of 2 reviews Showing All
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  • Transgelin/TAGLN/SM22 alpha Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult heart
    Species: Mouse
    Verified Customer | Posted 06/08/2017
    Coronary artery vascular smooth muscle cells in fixed, frozen adult mouse heart.
    Sample was fixed overnight in 4% PFA, blocked with 1% BSA; 1.5% Normal donkey serum; 0.1% triton X for 1 hour, ON with primary antibody 1:100 dilution of a 0.2mg/ml solution, washed, stained 1 hour with gt antisheep antibody 1:500 concentration.
    Transgelin/TAGLN/SM22 alpha Antibody - BSA Free NBP2-20405
  • Transgelin/TAGLN/SM22 alpha Antibody
    Name: Michelle Tallquist
    Application: Immunohistochemistry
    Sample Tested: Adult heart
    Species: Mouse
    Verified Customer | Posted 05/03/2017
    40X image of coronary artery vascular smooth muscle stained for transgelin.
    Fixed o.n. 4% PFA, block 1 hour 1%BSA in 0.1% Triton/PBS, 1:100 dilution antibody in 0.1% Triton/PBS o.n., 1 hour secondary.
    Transgelin/TAGLN/SM22 alpha Antibody - BSA Free NBP2-20405

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