Transgelin/TAGLN/SM22 alpha Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-20405
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Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine
Predicted:
Porcine (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Transgelin/TAGLN/SM22 alpha. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (81%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Coronary artery vascular smooth muscle cells in fixed, frozen adult mouse heart. Primary antibody 1:100 dilution of a 0.2mg/ml solution. This image was submitted via customer Review.Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Paraffin-embedded mouse esophagus. Transgelin antibody dilution: 1:500.Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405]
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - 40X image of coronary artery vascular smooth muscle stained for transgelin. Primary Antibody dilution of 1:100. This image was submitted via customer Review.Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin/TAGLN/SM22 alpha antibody (NBP2-20405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Transgelin/TAGLN/SM22 alpha antibody detects Transgelin/TAGLN/SM22 alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.
Transgelin/TAGLN/SM22 alpha stained by Transgelin/TAGLN/SM22 alpha antibody (NBP2-20405) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -
Transgelin antibody detects Transgelin protein at cytosol on mouse heart by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.
Transgelin antibody (NBP2-20405) dilution: 1:500.
Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP2-20405] -
Transgelin antibody detects Transgelin protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin protein stained by Transgelin antibody (NBP2-20405) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500.
Blue: Hoechst 33342 staining.
Applications for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:10000
Reviewed Applications
Read 2 reviews rated 3 using NBP2-20405 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Transgelin/TAGLN
Additional Transgelin/TAGLN Products
Product Documents for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Transgelin/TAGLN/SM22 alpha Antibody - BSA Free (2)
3 out of 5
2 Customer Ratings
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Customer Images
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Application: ImmunohistochemistrySample Tested: Adult heartSpecies: MouseVerified Customer | Posted 06/08/2017Coronary artery vascular smooth muscle cells in fixed, frozen adult mouse heart.Sample was fixed overnight in 4% PFA, blocked with 1% BSA; 1.5% Normal donkey serum; 0.1% triton X for 1 hour, ON with primary antibody 1:100 dilution of a 0.2mg/ml solution, washed, stained 1 hour with gt antisheep antibody 1:500 concentration.
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Application: ImmunohistochemistrySample Tested: Adult heartSpecies: MouseVerified Customer | Posted 05/03/201740X image of coronary artery vascular smooth muscle stained for transgelin.Fixed o.n. 4% PFA, block 1 hour 1%BSA in 0.1% Triton/PBS, 1:100 dilution antibody in 0.1% Triton/PBS o.n., 1 hour secondary.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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