TRAP1 Antibody

Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody [NBP2-20700] -

TRAP1 antibody [N1N3] detects TRAP1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and permeabilized with 100% MeOH for 30 sec.
Green: TRAP1 protein stained by TRAP1 antibody [N1N3] (NBP2-20700) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Western Blot: TRAP1 Antibody [NBP2-20700] -

Combined inhibition of TRAP1 and HDACs enhanced activation of a cell death with apoptotic features, including cleavage of caspases. (A) U87 and LN229 cells were treated with the indicated concentration of gamitrinib, panobinostat or the combination of both for 48h. Thereafter, cells were labeled with annexin/propidium iodide (PI) dye and analyzed by multi-parametric flow cytometry. Shown are representative flow plots; (B,C) U87 and LN229 cells were treated with the indicated concentrations of gamitrinib, panobinostat/romidepsin or the combination of both for 48h. Thereafter, cells were labeled with propidium iodide (PI) dye and analyzed by flow cytometry. Shown are representative flow plots; (D) Standard western blots of cell lysates of U87 and T98G treated with gamitrinib, panobinostat or the combination of both for 24 h. Tubulin is used as a loading control. FL: full length, CF: cleaved fragment; (E) U87 and T98G cells were treated with the combination treatment of gamitrinib and panobinostat in the presence or absence of zVAD for 24h. Thereafter, cells were labeled with propidium iodide (PI) dye and analyzed by flow cytometry. Shown are representative flow plots; (F–H) U87 GBM cells were transfected with scrambled or TRAP1 specific siRNA and treated with panobinostat for 24h. Knockdown efficiency was confirmed by protein capillary electrophoresis. Vinculin serves as a loading control. Thereafter, cells were labeled with Annexin/PI dye and analyzed by multi-parametric flow cytometry (n = 3). Shown are means and SD. Statistical significance was determined by two-tailed Student’s t-test. **** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32664214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.