TRF-2 Antibody (4A794.15) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-33212

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Key Product Details

Species Reactivity

Human, Mouse, Rat, Deer, Marsupial

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4A794.15

Format

Azide and BSA Free
View all TRF-2 Primary Antibodies »

Product Specifications

Immunogen

This TRF-2 Antibody (4A794.15) - Azide Free was developed against Baculovirus expressed whole length TRF2 protein, used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).

Marker

Telomeres marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRF-2 Antibody (4A794.15) - Azide and BSA Free

Western Blot: TRF-2 Antibody (4A794.15)Azide Free [NBP2-33212]

Western Blot: TRF-2 Antibody (4A794.15)Azide Free [NBP2-33212]

Western Blot: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - Total protein from mouse 3T3 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TRF2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - Staining of TRF2-bound telomeres in human HeLa cells (Courtesy of Fotiadou, et al, 2004). Image from the standard format of this antibody.
Immunohistochemistry: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunohistochemistry: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunohistochemistry: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - Transitional cell carcinoma, urinary bladder, stained with TRF2 antibody (4 ug/mL), peroxidase-conjugate and DAB chromogen. Note specific nuclear staining. Tumor/normal adjacent tissue array slide was used for this test. Staining of formalin-fixed tissues
Flow Cytometry: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Flow Cytometry: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Flow Cytometry: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - Intracellular flow cytometric analysis of TRF2 in 10^6 human Jurkat cells using 0.1 ug of NB100-56506. The shaded histogram represents cells alone, blue represents isotype control and red represents NB100-56506, anti-TRF2. Image from the standard format of this antibody.
Western Blot: TRF-2 Antibody (4A794.15)Azide Free [NBP2-33212]

Western Blot: TRF-2 Antibody (4A794.15)Azide Free [NBP2-33212]

Western Blot: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - Analysis in human Jurkat cell lysate at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TRF-2 (4A794.15) conjugated to FITC [NB100-56506F] at 5 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - Localization of candidate RNAs at telomeres. Localization of NEAT1 at telomeres in human U-2 OS cells. Cells were fixed and sequentially incubated with Abs against TRF-2 and AlexaFluor 488-conjugated anti-mouse IgG. Subsequently, the cells were hybridized with the RNA probes and subjected to fluorescence microscopy. Three different cells are shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0123387), licensed under a CC-BY license. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212]

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - Localization of candidate RNAs at telomeres. Localization of SNORD17 at telomeres in human U-2 OS cells. Cells were fixed and sequentially incubated with Abs against TRF-2 and AlexaFluor 488-conjugated anti-mouse IgG. Subsequently, the cells were hybridized with the RNA probes and subjected to fluorescence microscopy. Three different cells are shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0123387), licensed under a CC-BY license. Image from the standard format of this antibody.
Simple Western: TRF-2 Antibody (4A794.15)Azide Free [NBP2-33212]

Simple Western: TRF-2 Antibody (4A794.15)Azide Free [NBP2-33212]

Simple Western: TRF-2 Antibody (4A794.15) - Azide Free [NBP2-33212] - Image shows a specific band for TRF2 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from the standard format of this antibody.

Applications for TRF-2 Antibody (4A794.15) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

2 ug/ml

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

5 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2 ug/10^6 cells

Western Blot

2-4 ug/ml
Application Notes
1. TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. However, observed molecular weights could vary depending on molecular weight standards used and gel conditions. 2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. 3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information. 4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRF-2

Originally discovered as part of shelterin complex, telomeric repeat-binding factor 2 (TRF2, also called TERF2 or TRB2) is a ubiquitously expressed nuclear protein (55-60 kDa) involved in telomere homeostasis. TRF2 contains an N-terminal GAR domain, a central TRFH dimerization domain, and a C-terminal SAND/MYB-type DNA binding domain. Trf2 RNA has 10 exons and alternative splicing in rodents produces a truncated form, TRF2-S, which lacks the DNA binding domain and nuclear localization signal (NLS) (1).

Both TRF2 and TRF1 bind to telomeric double stranded 5'-TTAGGG-3' DNA repeats, then recruit RAP1, TIN2, TPP1, and POT1 for the assembly of the shelterin complex. The telomeric association of TRF2 is greatly increased in the S phase of the cell cycle (2). Loss of TRF2 leads to telomere shortening, the DNA damage response, chromosomal instability, and replicative senescence. Interestingly, the contribution of TRF2 to telomere shortening via a telomerase-independent mechanism has also been reported (3). In conjunction with the exonuclease, Apollo, TRF2 protects telomeres during replication and negatively regulates the accumulation of DNA topoisomerase (TOP1, TOP2A and TOP2B).

TRF2 has been implicated in cancer, shown to be a major oncogene in telomerase-deficient mice. A link to Werner syndrome, a premature aging disease caused by the loss of WRN, has been reported based on TRF2 recruitment of WRN for processing of telomeric DNA (4). TRF2 expression is increased during human embryonic stem cell differentiation and has been shown to interact with Repressor Element-1 Silencing Transcription Factor (REST), protecting it from proteasomal degradation (5).

References

1. Grammatikakis, I., Zhang, P., Mattson, M. P., & Gorospe, M. (2016). The long and the short of TRF2 in neurogenesis. Cell cycle (Georgetown, Tex.), 15(22), 3026-3032. PMID: 27565210

2. Li, F., Kim, H., Ji, Z., Zhang, T., Chen, B., Ge, Y., Hu, Y., Feng, X., Han, X., Xu, H., Zhang, Y., Yu, H., Liu, D., Ma, W., & Songyang, Z. (2018). The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Molecular cell, 70(3), 395-407. PMID: 29727616

3. Ancelin, K., Brunori, M., Bauwens, S., Koering, C. E., Brun, C., Ricoul, M., Pommier, J. P., Sabatier, L., & Gilson, E. (2002). Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2. Molecular and cellular biology, 22(10), 3474-3487. PMID: 11971978

4. Machwe A, Xiao L, & Orren DK. (2004) TRF2 recruits the Werner syndrome (WRN) exonuclease for processing of telomeric DNA. Oncogene. 23(1):149-56. PMID: 14712220.

5. Diotti, R., & Loayza, D. (2011). Shelterin complex and associated factors at human telomeres. Nucleus (Austin, Tex.), 2(2), 119-135. PMID: 21738835

Long Name

Telomeric Repeat Binding Factor 2

Alternate Names

TERF2, TRBF2, TRF2

Gene Symbol

TERF2

Additional TRF-2 Products

Product Documents for TRF-2 Antibody (4A794.15) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TRF-2 Antibody (4A794.15) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

p53 Pathway

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TRF-2 Antibody (4A794.15) - Azide and BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Are the TRF-2 antibodies validated in Simple Western?

    A: Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.

  • Q: Does TRF-2 antibodies comes in lyophilized form?

    A: we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.

  • Q: What is the immunogen sequence of this TRF-2 antibody?

    A: The whole length of Baculovirus expressed His-tagged TRF-2 protein is proprietary.

  • Q: What the theoretical molecular weight for TRF-2 antibodies?

    A: The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.

  • Q: Are the TRF-2 antibodies validated in Simple Western?

    A: Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.

  • Q: Does TRF-2 antibodies comes in lyophilized form?

    A: we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.

  • Q: What is the immunogen sequence of this TRF-2 antibody?

    A: The whole length of Baculovirus expressed His-tagged TRF-2 protein is proprietary.

  • Q: What the theoretical molecular weight for TRF-2 antibodies?

    A: The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.

  • Q: Are the TRF-2 antibodies validated in Simple Western?

    A: Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.

  • Q: Does TRF-2 antibodies comes in lyophilized form?

    A: we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.

  • Q: What is the immunogen sequence of this TRF-2 antibody?

    A: The whole length of Baculovirus expressed His-tagged TRF-2 protein is proprietary.

  • Q: What the theoretical molecular weight for TRF-2 antibodies?

    A: The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.

  • Q: Are the TRF-2 antibodies validated in Simple Western?

    A: Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.

  • Q: Does TRF-2 antibodies comes in lyophilized form?

    A: we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.

  • Q: What is the immunogen sequence of this TRF-2 antibody?

    A: The whole length of Baculovirus expressed His-tagged TRF-2 protein is proprietary.

  • Q: What the theoretical molecular weight for TRF-2 antibodies?

    A: The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.

Showing  1 - 4 of 4 FAQs Showing All
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