TRIM25 Antibody (5B5B12) - BSA Free

Novus Biologicals | Catalog # NBP2-61814

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 5B5B12

Format

BSA Free
View all TRIM25 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human TRIM25 (AA: 211-360) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM25 Antibody (5B5B12) - BSA Free

Western Blot: TRIM25 Antibody (5B5B12)BSA Free [NBP2-61814]

Western Blot: TRIM25 Antibody (5B5B12)BSA Free [NBP2-61814]

Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

Immunohistochemistry: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded rectum cancer tissues using TRIM25 mouse mAb with DAB staining.
Western Blot: TRIM25 Antibody (5B5B12)BSA Free [NBP2-61814]

Western Blot: TRIM25 Antibody (5B5B12)BSA Free [NBP2-61814]

Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)
Western Blot: TRIM25 Antibody (5B5B12)BSA Free [NBP2-61814]

Western Blot: TRIM25 Antibody (5B5B12)BSA Free [NBP2-61814]

Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.
Immunohistochemistry: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

Immunohistochemistry: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining.
ELISA: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

ELISA: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
ELISA: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

ELISA: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814]

ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).
TRIM25 Antibody (5B5B12) - BSA Free

Western Blot: TRIM25 Antibody (5B5B12) - BSA Free [NBP2-61814] -

K63-Ubiquitinated FMR1 undergoes ESCRT-dependent EV loading. (A) Pulldown of FMR1 shows that it interacts with Hrs after inflammasome activation. This interaction is lost when TRIM25 is silenced (shT25). (B-C) Knockdown of TRIM25 does not alter the number of EVs secreted or the contents of known exosome markers. EV numbers were quantified via Bradford dye as performed by us previously15. (D) Silencing TRIM25 dramatically reduces the EV enrichment of FMR1 during inflammation. (E) Correlation between the amount of intracellular TRIM25 and the amount of EV-associated FMR1. The amount of intracellular TRIM25 is positively correlated with EV FMR1. THP-1 cells were used for this figure. Data are shown as mean +/- SEM; *p ≤ 0.05 for n = 3–5. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38135735), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TRIM25 Antibody (5B5B12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM25

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.;

Long Name

Tripartite Motif-containing Protein 25

Alternate Names

EFP, RNF147, ZNF147

Gene Symbol

TRIM25

Additional TRIM25 Products

Product Documents for TRIM25 Antibody (5B5B12) - BSA Free

Certificate of Analysis

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Product Specific Notices for TRIM25 Antibody (5B5B12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRIM25 Antibody (5B5B12) - BSA Free

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Protocols

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