TRIM29 Antibody

Novus Biologicals | Catalog # NBP1-52000

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRIM29 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EKDRIKSFTTNE corresponding to internal region according to NP_036233.2.

Epitope

C-EKDRIKSFTTNE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRIM29 Antibody

Western Blot: TRIM29 Antibody [NBP1-52000]

Western Blot: TRIM29 Antibody [NBP1-52000]

Western Blot: TRIM29 Antibody [NBP1-52000] - (1ug/ml) staining of Peripheral Blood Mononucleocyte (A) and (0.1 ug/ml) HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: TRIM29 Antibody [NBP1-52000]

Immunocytochemistry/ Immunofluorescence: TRIM29 Antibody [NBP1-52000]

Immunocytochemistry/Immunofluorescence: TRIM29 Antibody [NBP1-52000] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and Intermediate filament staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-52000]

Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-52000]

Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-52000] - (3.8ug/ml) staining of Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: TRIM29 Antibody [NBP1-52000]

Flow Cytometry: TRIM29 Antibody [NBP1-52000]

Flow Cytometry: TRIM29 Antibody [NBP1-52000] - analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: TRIM29 Antibody [NBP1-52000]

Immunocytochemistry/ Immunofluorescence: TRIM29 Antibody [NBP1-52000]

Immunocytochemistry/Immunofluorescence: TRIM29 Antibody [NBP1-52000] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Applications for TRIM29 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1-1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 65.8 kDa band according to NP_036233.2). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room tempetature.
IHC: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes.
Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filament of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells.
Flow Cytometry: Flow cytometric analysis of A431 cells.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM29

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype.

Alternate Names

Ataxia telangiectasia group D-associated protein, ATDC, ATDC ataxia-telangiectasia group D-associated protein, FLJ36085, tripartite motif containing 29, tripartite motif protein TRIM29, tripartite motif-containing 29, tripartite motif-containing protein 29

Entrez Gene IDs

23650 (Human); 72169 (Mouse); 300656 (Rat)

Gene Symbol

TRIM29

UniProt

NP_036233.2 (Human)

Additional TRIM29 Products

Product Documents for TRIM29 Antibody

Certificate of Analysis

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Product Specific Notices for TRIM29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRIM29 Antibody

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Protocols

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