TRIM30 Antibody - BSA Free

Western Blot: TRIM30 Antibody - BSA Free [NBP2-41087] -

Trim30a is upregulated by H. pylori in a STING-dependent manner. Murine gastric organoid monolayers or bone marrow derived dendritic cells (BMDC) derived from wild-type (WT) C57BL/6 or Sting−/− mice were challenged with PBS alone (UI) or wild-type (wt) H. pylori strain J166 or PMSS1 at MOI 100:1 for 24 hours. (a) Trim30a was quantified by Western blot analysis in co-cultured murine gastric organoid. Representative Western blots and densitometric analysis normalizing levels of Trim30a to Gapdh. (b) RT-PCR analysis of Trim30a mRNA levels in uninfected and H. pylori-infected wild-type and Sting−/− BMDCs. Data are represented as relative gene expression normalized to levels of Gapdh gene expression. (c) Trim30a was quantified by Western blot analysis in co-cultured BMDCs. Representative Western blots and densitometric analysis normalizing levels of Trim30a to Gapdh. In each experiment, conditions were tested at least 3 times and student’s t-tests were used to determine statistical significance between groups. *p <.05, **p <.01, ns = not significant. ##p <.01, ###p <.001 ####p <.0001 compared to untreated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35905376), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TRIM30 Antibody - BSA Free [NBP2-41087] -

Trim30a is upregulated by H. pylori in a STING-dependent manner. Murine gastric organoid monolayers or bone marrow derived dendritic cells (BMDC) derived from wild-type (WT) C57BL/6 or Sting−/− mice were challenged with PBS alone (UI) or wild-type (wt) H. pylori strain J166 or PMSS1 at MOI 100:1 for 24 hours. (a) Trim30a was quantified by Western blot analysis in co-cultured murine gastric organoid. Representative Western blots and densitometric analysis normalizing levels of Trim30a to Gapdh. (b) RT-PCR analysis of Trim30a mRNA levels in uninfected and H. pylori-infected wild-type and Sting−/− BMDCs. Data are represented as relative gene expression normalized to levels of Gapdh gene expression. (c) Trim30a was quantified by Western blot analysis in co-cultured BMDCs. Representative Western blots and densitometric analysis normalizing levels of Trim30a to Gapdh. In each experiment, conditions were tested at least 3 times and student’s t-tests were used to determine statistical significance between groups. *p <.05, **p <.01, ns = not significant. ##p <.01, ###p <.001 ####p <.0001 compared to untreated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35905376), licensed under a CC-BY license. Not internally tested by Novus Biologicals.